A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4
Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2021-08-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-NT-03-2021-0047.pdf |
| _version_ | 1811214611619250176 |
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| author | Da-Ran Kim Youn-Sig Kwak |
| author_facet | Da-Ran Kim Youn-Sig Kwak |
| author_sort | Da-Ran Kim |
| collection | DOAJ |
| description | Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or antibacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lantipeptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture. |
| first_indexed | 2024-04-12T06:07:31Z |
| format | Article |
| id | doaj.art-ba8447c54c6b4bb788f19df1b15c327b |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-12T06:07:31Z |
| publishDate | 2021-08-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-ba8447c54c6b4bb788f19df1b15c327b2022-12-22T03:44:50ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-08-0137438939510.5423/PPJ.NT.03.2021.00472263A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4Da-Ran Kim0Youn-Sig Kwak1 Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea Research Institute of Life Science, Gyeongsang National University, Jinju 52828, KoreaSoil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or antibacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lantipeptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture.http://www.ppjonline.org/upload/pdf/PPJ-NT-03-2021-0047.pdfantifungalantibacteriallantibioticlassopeptide |
| spellingShingle | Da-Ran Kim Youn-Sig Kwak A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4 The Plant Pathology Journal antifungal antibacterial lantibiotic lassopeptide |
| title | A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4 |
| title_full | A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4 |
| title_fullStr | A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4 |
| title_full_unstemmed | A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4 |
| title_short | A Genome-Wide Analysis of Antibiotic Producing Genes in SP6C4 |
| title_sort | genome wide analysis of antibiotic producing genes in sp6c4 |
| topic | antifungal antibacterial lantibiotic lassopeptide |
| url | http://www.ppjonline.org/upload/pdf/PPJ-NT-03-2021-0047.pdf |
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