Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating
Human pluripotent stem cells (hPSCs) are ideal “raw materials” for making various human cell types for regenerative medicine and are needed in large numbers. 3D suspension culturing (e.g., stirred-tank bioreactor or STR), which suspends and cultures cells in an agitated medium, has been extensively...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Chemical Engineering |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fceng.2022.1031395/full |
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| author | Qiang Li Ying Pan Li Han Yakun Yang Xinran Wu Yuguo Lei Yuguo Lei Yuguo Lei |
| author_facet | Qiang Li Ying Pan Li Han Yakun Yang Xinran Wu Yuguo Lei Yuguo Lei Yuguo Lei |
| author_sort | Qiang Li |
| collection | DOAJ |
| description | Human pluripotent stem cells (hPSCs) are ideal “raw materials” for making various human cell types for regenerative medicine and are needed in large numbers. 3D suspension culturing (e.g., stirred-tank bioreactor or STR), which suspends and cultures cells in an agitated medium, has been extensively studied to scale up hPSC production. However, a significant problem with 3D suspension is the uncontrolled spheroid agglomeration. It leads to cell growth arrest, cell apoptosis, and inhomogeneity in cell purity and quality. We propose that i) inhibiting the spheroid adhesion can prevent spheroid agglomeration and ii) the inhibition can be achieved via coating spheroids with biocompatible anti-adhesion molecules. We used PEG-lipids as model anti-adhesion molecules to successfully demonstrate the concept. PEG-lipids anchor to the spheroid surface through the interactions between their lipid chains and the cell membrane lipids. The flexible and hydrophilic PEG chains act as a dynamic barrier to prevent spheroid adhesion. We showed that the coating eliminated spheroid agglomeration, leading to homogenous spheroid size distribution and significant improvements in cell growth rate and volumetric yield. This novel approach is expected to impact large-scale hPSC production significantly. Furthermore, the approach can be generalized for culturing other human cell types. |
| first_indexed | 2024-04-12T16:29:32Z |
| format | Article |
| id | doaj.art-ba85e98a7dfa496e9e1b643c3c998bd0 |
| institution | Directory Open Access Journal |
| issn | 2673-2718 |
| language | English |
| last_indexed | 2024-04-12T16:29:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemical Engineering |
| spelling | doaj.art-ba85e98a7dfa496e9e1b643c3c998bd02022-12-22T03:25:14ZengFrontiers Media S.A.Frontiers in Chemical Engineering2673-27182022-10-01410.3389/fceng.2022.10313951031395Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coatingQiang Li0Ying Pan1Li Han2Yakun Yang3Xinran Wu4Yuguo Lei5Yuguo Lei6Yuguo Lei7Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesDepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesHuck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, United StatesHuman pluripotent stem cells (hPSCs) are ideal “raw materials” for making various human cell types for regenerative medicine and are needed in large numbers. 3D suspension culturing (e.g., stirred-tank bioreactor or STR), which suspends and cultures cells in an agitated medium, has been extensively studied to scale up hPSC production. However, a significant problem with 3D suspension is the uncontrolled spheroid agglomeration. It leads to cell growth arrest, cell apoptosis, and inhomogeneity in cell purity and quality. We propose that i) inhibiting the spheroid adhesion can prevent spheroid agglomeration and ii) the inhibition can be achieved via coating spheroids with biocompatible anti-adhesion molecules. We used PEG-lipids as model anti-adhesion molecules to successfully demonstrate the concept. PEG-lipids anchor to the spheroid surface through the interactions between their lipid chains and the cell membrane lipids. The flexible and hydrophilic PEG chains act as a dynamic barrier to prevent spheroid adhesion. We showed that the coating eliminated spheroid agglomeration, leading to homogenous spheroid size distribution and significant improvements in cell growth rate and volumetric yield. This novel approach is expected to impact large-scale hPSC production significantly. Furthermore, the approach can be generalized for culturing other human cell types.https://www.frontiersin.org/articles/10.3389/fceng.2022.1031395/fullhuman pluripotent stem cellsthree-dimensional culturesurface coatingsurfactantpoly (ethylene glycol) |
| spellingShingle | Qiang Li Ying Pan Li Han Yakun Yang Xinran Wu Yuguo Lei Yuguo Lei Yuguo Lei Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating Frontiers in Chemical Engineering human pluripotent stem cells three-dimensional culture surface coating surfactant poly (ethylene glycol) |
| title | Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating |
| title_full | Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating |
| title_fullStr | Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating |
| title_full_unstemmed | Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating |
| title_short | Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating |
| title_sort | improving three dimensional human pluripotent cell culture efficiency via surface molecule coating |
| topic | human pluripotent stem cells three-dimensional culture surface coating surfactant poly (ethylene glycol) |
| url | https://www.frontiersin.org/articles/10.3389/fceng.2022.1031395/full |
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