Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure
The preclinical research conducted so far suggest that depression development may be influenced by the inflammatory pathways both at the periphery and within the central nervous system. Furthermore, inflammation is considered to be strongly connected with antidepressant treatment resistance. Thus, t...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/8983 |
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| author | Katarzyna Bialek Piotr Czarny Paulina Wigner Ewelina Synowiec Lukasz Kolodziej Michal Bijak Janusz Szemraj Mariusz Papp Tomasz Sliwinski |
| author_facet | Katarzyna Bialek Piotr Czarny Paulina Wigner Ewelina Synowiec Lukasz Kolodziej Michal Bijak Janusz Szemraj Mariusz Papp Tomasz Sliwinski |
| author_sort | Katarzyna Bialek |
| collection | DOAJ |
| description | The preclinical research conducted so far suggest that depression development may be influenced by the inflammatory pathways both at the periphery and within the central nervous system. Furthermore, inflammation is considered to be strongly connected with antidepressant treatment resistance. Thus, this study explores whether the chronic mild stress (CMS) procedure and agomelatine treatment induce changes in <i>TGFA</i>, <i>TGFB</i>, <i>IRF1</i>, <i>PTGS2</i> and <i>IKBKB</i> expression and methylation status in peripheral blood mononuclear cells (PBMCs) and in the brain structures of rats. Adult male Wistar rats were subjected to the CMS and further divided into matched subgroups to receive vehicle or agomelatine. TaqMan gene expression assay and methylation-sensitive high-resolution melting (MS-HRM) were used to evaluate the expression of the genes and the methylation status of their promoters, respectively. Our findings confirm that both CMS and antidepressant agomelatine treatment influenced the expression level and methylation status of the promoter region of investigated genes in PBMCs and the brain. What is more, the present study showed that response to either stress stimuli or agomelatine differed between brain structures. Concluding, our results indicate that <i>TGFA</i>, <i>TGFB</i>, <i>PTGS2</i>, <i>IRF1</i> and <i>IKBKB</i> could be associated with depression and its treatment. |
| first_indexed | 2024-03-09T04:21:14Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:21:14Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ba894ecc180647aa9be57e07a411d4682023-12-03T13:47:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316898310.3390/ijms23168983Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress ProcedureKatarzyna Bialek0Piotr Czarny1Paulina Wigner2Ewelina Synowiec3Lukasz Kolodziej4Michal Bijak5Janusz Szemraj6Mariusz Papp7Tomasz Sliwinski8Department of Medical Biochemistry, Medical University of Lodz, 92-215 Lodz, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-215 Lodz, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandLaboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-215 Lodz, PolandBiohazard Prevention Centre, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-215 Lodz, PolandInstitute of Pharmacology, Polish Academy of Sciences, 31-343 Krakow, PolandLaboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandThe preclinical research conducted so far suggest that depression development may be influenced by the inflammatory pathways both at the periphery and within the central nervous system. Furthermore, inflammation is considered to be strongly connected with antidepressant treatment resistance. Thus, this study explores whether the chronic mild stress (CMS) procedure and agomelatine treatment induce changes in <i>TGFA</i>, <i>TGFB</i>, <i>IRF1</i>, <i>PTGS2</i> and <i>IKBKB</i> expression and methylation status in peripheral blood mononuclear cells (PBMCs) and in the brain structures of rats. Adult male Wistar rats were subjected to the CMS and further divided into matched subgroups to receive vehicle or agomelatine. TaqMan gene expression assay and methylation-sensitive high-resolution melting (MS-HRM) were used to evaluate the expression of the genes and the methylation status of their promoters, respectively. Our findings confirm that both CMS and antidepressant agomelatine treatment influenced the expression level and methylation status of the promoter region of investigated genes in PBMCs and the brain. What is more, the present study showed that response to either stress stimuli or agomelatine differed between brain structures. Concluding, our results indicate that <i>TGFA</i>, <i>TGFB</i>, <i>PTGS2</i>, <i>IRF1</i> and <i>IKBKB</i> could be associated with depression and its treatment.https://www.mdpi.com/1422-0067/23/16/8983depressionchronic mild stressagomelatineinflammationexpressionmethylation |
| spellingShingle | Katarzyna Bialek Piotr Czarny Paulina Wigner Ewelina Synowiec Lukasz Kolodziej Michal Bijak Janusz Szemraj Mariusz Papp Tomasz Sliwinski Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure International Journal of Molecular Sciences depression chronic mild stress agomelatine inflammation expression methylation |
| title | Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure |
| title_full | Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure |
| title_fullStr | Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure |
| title_full_unstemmed | Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure |
| title_short | Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure |
| title_sort | agomelatine changed the expression and methylation status of inflammatory genes in blood and brain structures of male wistar rats after chronic mild stress procedure |
| topic | depression chronic mild stress agomelatine inflammation expression methylation |
| url | https://www.mdpi.com/1422-0067/23/16/8983 |
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