In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death
Introduction: Duchenne muscular dystrophy (DMD) is a progressive disease that leads to damage of muscle and myocardium due to genetic abnormalities in the dystrophin gene. In utero cell transplantation that might facilitate allogenic transplantation is worth considering to treat this disease. Method...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320422001006 |
| _version_ | 1811188686706966528 |
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| author | Yuki Kihara Yukie Tanaka Masanari Ikeda Jun Homma Ryo Takagi Keiko Ishigaki Keitaro Yamanouchi Hiroaki Honda Satoru Nagata Masayuki Yamato |
| author_facet | Yuki Kihara Yukie Tanaka Masanari Ikeda Jun Homma Ryo Takagi Keiko Ishigaki Keitaro Yamanouchi Hiroaki Honda Satoru Nagata Masayuki Yamato |
| author_sort | Yuki Kihara |
| collection | DOAJ |
| description | Introduction: Duchenne muscular dystrophy (DMD) is a progressive disease that leads to damage of muscle and myocardium due to genetic abnormalities in the dystrophin gene. In utero cell transplantation that might facilitate allogenic transplantation is worth considering to treat this disease. Methods: We performed allogeneic in utero transplantation of GFP-positive myoblasts and adipose-derived mesenchymal stem cells into murine DMD model animals. The transplantation route in this study was fetal intraperitoneal transplantation and transplacental transplantation. Transplanted animals were examined at 4-weeks old by immunofluorescence staining and RT-qPCR. Results: No GFP-positive cells were found by immunofluorescence staining of skeletal muscle and no GFP mRNA was detected by RT-qPCR in any animal, transplantation method and cell type. Compared with previous reports, myoblast transplantation exhibited an equivalent mortality rate, but adipose-derived stem cell (ASC) transplantation produced a higher mortality rate. Conclusions: In utero transplantation of myoblasts or ASCs to murine models of DMD does not lead to engraftment and, in ASC transplantation primarily, frequently results in fetal death. |
| first_indexed | 2024-04-11T14:22:30Z |
| format | Article |
| id | doaj.art-ba8eb31e37f24328b7308889df383856 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-04-11T14:22:30Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-ba8eb31e37f24328b7308889df3838562022-12-22T04:19:00ZengElsevierRegenerative Therapy2352-32042022-12-0121486493In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal deathYuki Kihara0Yukie Tanaka1Masanari Ikeda2Jun Homma3Ryo Takagi4Keiko Ishigaki5Keitaro Yamanouchi6Hiroaki Honda7Satoru Nagata8Masayuki Yamato9Department of Pediatrics, Tokyo Women’s Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JapanDepartment of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, JapanDepartment of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JapanDepartment of Pediatrics, Tokyo Women’s Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JapanDepartment of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, JapanHuman Disease Models, Institute of Laboratory Animals, Tokyo Women's Medical University, Tokyo, JapanDepartment of Pediatrics, Tokyo Women’s Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 Japan; Corresponding author. Fax: +81 3-3359-6046.Introduction: Duchenne muscular dystrophy (DMD) is a progressive disease that leads to damage of muscle and myocardium due to genetic abnormalities in the dystrophin gene. In utero cell transplantation that might facilitate allogenic transplantation is worth considering to treat this disease. Methods: We performed allogeneic in utero transplantation of GFP-positive myoblasts and adipose-derived mesenchymal stem cells into murine DMD model animals. The transplantation route in this study was fetal intraperitoneal transplantation and transplacental transplantation. Transplanted animals were examined at 4-weeks old by immunofluorescence staining and RT-qPCR. Results: No GFP-positive cells were found by immunofluorescence staining of skeletal muscle and no GFP mRNA was detected by RT-qPCR in any animal, transplantation method and cell type. Compared with previous reports, myoblast transplantation exhibited an equivalent mortality rate, but adipose-derived stem cell (ASC) transplantation produced a higher mortality rate. Conclusions: In utero transplantation of myoblasts or ASCs to murine models of DMD does not lead to engraftment and, in ASC transplantation primarily, frequently results in fetal death.http://www.sciencedirect.com/science/article/pii/S2352320422001006Fetal therapyIn utero transplantationMyoblastsAdipose-derived mesenchymal stem cellsDuchenne muscular dystrophy |
| spellingShingle | Yuki Kihara Yukie Tanaka Masanari Ikeda Jun Homma Ryo Takagi Keiko Ishigaki Keitaro Yamanouchi Hiroaki Honda Satoru Nagata Masayuki Yamato In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death Regenerative Therapy Fetal therapy In utero transplantation Myoblasts Adipose-derived mesenchymal stem cells Duchenne muscular dystrophy |
| title | In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death |
| title_full | In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death |
| title_fullStr | In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death |
| title_full_unstemmed | In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death |
| title_short | In utero transplantation of myoblasts and adipose-derived mesenchymal stem cells to murine models of Duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death |
| title_sort | in utero transplantation of myoblasts and adipose derived mesenchymal stem cells to murine models of duchenne muscular dystrophy does not lead to engraftment and frequently results in fetal death |
| topic | Fetal therapy In utero transplantation Myoblasts Adipose-derived mesenchymal stem cells Duchenne muscular dystrophy |
| url | http://www.sciencedirect.com/science/article/pii/S2352320422001006 |
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