Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease
Ultraviolet-C (UV-C) irradiation of grains activated the antioxidant system and wheat seedlings’ resistance to leaf rust and powdery mildew disease under greenhouse conditions. Two wheat cultivars (Gemmeiza-12 and Sids-1) with dry and germinated grains were treated with UV-C at three exposure times...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Italian Journal of Agronomy |
| Subjects: | |
| Online Access: | https://agronomy.it/index.php/agro/article/view/2201 |
| _version_ | 1827090639790014464 |
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| author | Marian Thabet Mohamed A. Abou-Zeid Fatmah A. Safhi Khairiah M. Alwutayd Walaa Khalifa |
| author_facet | Marian Thabet Mohamed A. Abou-Zeid Fatmah A. Safhi Khairiah M. Alwutayd Walaa Khalifa |
| author_sort | Marian Thabet |
| collection | DOAJ |
| description |
Ultraviolet-C (UV-C) irradiation of grains activated the antioxidant system and wheat seedlings’ resistance to leaf rust and powdery mildew disease under greenhouse conditions. Two wheat cultivars (Gemmeiza-12 and Sids-1) with dry and germinated grains were treated with UV-C at three exposure times (5, 10, and 15 minutes). The results indicated that the percentages of disease severity and infection type for leaf rust and powdery mildew on wheat seedlings were significantly reduced when exposed to UVC at all exposure times compared to the untreated control. The most effective treatments for both cultivars were obtained in seedlings grown from germinated grains treated with UV-C for 10 minutes. Furthermore, UV-C irradiation treatments improved plant resistance to infection by activating certain defense genes, thereby increasing the production of resistance compounds that support defense mechanisms against pathogens. Our results demonstrated that UV-C for 10 minutes can induce resistance in wheat seedlings while also increasing total chlorophyll, total phenolic compounds, phenylalanine ammonia-lyase, and peroxidase activity. In addition, phenylalanine ammonia-lyase mRNA expression levels were significantly increased in seedlings growing from germinated grains treated with UV-C for 10 minutes, as compared to both infected and uninfected controls. These findings demonstrate the potential for additional UV-C radiation treatments to enhance disease resistance.
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| first_indexed | 2024-03-11T17:17:49Z |
| format | Article |
| id | doaj.art-ba8f7c131fc740d2bc7936ab92b33676 |
| institution | Directory Open Access Journal |
| issn | 1125-4718 2039-6805 |
| language | English |
| last_indexed | 2025-03-20T05:43:36Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Italian Journal of Agronomy |
| spelling | doaj.art-ba8f7c131fc740d2bc7936ab92b336762024-10-03T04:34:14ZengElsevierItalian Journal of Agronomy1125-47182039-68052023-10-0118310.4081/ija.2023.2201Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew diseaseMarian Thabet0Mohamed A. Abou-Zeid1Fatmah A. Safhi2Khairiah M. Alwutayd3Walaa Khalifa4Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, CairoWheat Diseases Research Department, Agricultural Research Center, Plant Pathology Research Institute, GizaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, RiyadhDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, RiyadhDepartment of Plant Pathology, Faculty of Agriculture, Ain Shams University, Cairo Ultraviolet-C (UV-C) irradiation of grains activated the antioxidant system and wheat seedlings’ resistance to leaf rust and powdery mildew disease under greenhouse conditions. Two wheat cultivars (Gemmeiza-12 and Sids-1) with dry and germinated grains were treated with UV-C at three exposure times (5, 10, and 15 minutes). The results indicated that the percentages of disease severity and infection type for leaf rust and powdery mildew on wheat seedlings were significantly reduced when exposed to UVC at all exposure times compared to the untreated control. The most effective treatments for both cultivars were obtained in seedlings grown from germinated grains treated with UV-C for 10 minutes. Furthermore, UV-C irradiation treatments improved plant resistance to infection by activating certain defense genes, thereby increasing the production of resistance compounds that support defense mechanisms against pathogens. Our results demonstrated that UV-C for 10 minutes can induce resistance in wheat seedlings while also increasing total chlorophyll, total phenolic compounds, phenylalanine ammonia-lyase, and peroxidase activity. In addition, phenylalanine ammonia-lyase mRNA expression levels were significantly increased in seedlings growing from germinated grains treated with UV-C for 10 minutes, as compared to both infected and uninfected controls. These findings demonstrate the potential for additional UV-C radiation treatments to enhance disease resistance. https://agronomy.it/index.php/agro/article/view/2201Wheatleaf rustpowdery mildewUVphenolic compoundsenzyme activity |
| spellingShingle | Marian Thabet Mohamed A. Abou-Zeid Fatmah A. Safhi Khairiah M. Alwutayd Walaa Khalifa Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease Italian Journal of Agronomy Wheat leaf rust powdery mildew UV phenolic compounds enzyme activity |
| title | Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease |
| title_full | Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease |
| title_fullStr | Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease |
| title_full_unstemmed | Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease |
| title_short | Ultraviolet-C irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease |
| title_sort | ultraviolet c irradiation of wheat grains induces seedling resistance to leaf rust and powdery mildew disease |
| topic | Wheat leaf rust powdery mildew UV phenolic compounds enzyme activity |
| url | https://agronomy.it/index.php/agro/article/view/2201 |
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