Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy
We analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is po...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2013-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/101.pdf |
| _version_ | 1827606389199994880 |
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| author | Victor P. Kutyshenko Petr M. Beskaravayny Maxim V. Molchanov Svetlana I. Paskevich Dmitry A. Prokhorov Vladimir N. Uversky |
| author_facet | Victor P. Kutyshenko Petr M. Beskaravayny Maxim V. Molchanov Svetlana I. Paskevich Dmitry A. Prokhorov Vladimir N. Uversky |
| author_sort | Victor P. Kutyshenko |
| collection | DOAJ |
| description | We analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics. |
| first_indexed | 2024-03-09T06:37:33Z |
| format | Article |
| id | doaj.art-ba8fc430b5444066873331d8d761b8ca |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:37:33Z |
| publishDate | 2013-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-ba8fc430b5444066873331d8d761b8ca2023-12-03T10:56:17ZengPeerJ Inc.PeerJ2167-83592013-07-011e10110.7717/peerj.101101Looking at microbial metabolism by high-resolution 2H-NMR spectroscopyVictor P. Kutyshenko0Petr M. Beskaravayny1Maxim V. Molchanov2Svetlana I. Paskevich3Dmitry A. Prokhorov4Vladimir N. Uversky5Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, RussiaInstitute for Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region, RussiaWe analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.https://peerj.com/articles/101.pdfMicrobe adaptationMicrobial metabolism2H-HMR spectroscopyHigh resolution NMRHeavy water |
| spellingShingle | Victor P. Kutyshenko Petr M. Beskaravayny Maxim V. Molchanov Svetlana I. Paskevich Dmitry A. Prokhorov Vladimir N. Uversky Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy PeerJ Microbe adaptation Microbial metabolism 2H-HMR spectroscopy High resolution NMR Heavy water |
| title | Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy |
| title_full | Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy |
| title_fullStr | Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy |
| title_full_unstemmed | Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy |
| title_short | Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy |
| title_sort | looking at microbial metabolism by high resolution 2h nmr spectroscopy |
| topic | Microbe adaptation Microbial metabolism 2H-HMR spectroscopy High resolution NMR Heavy water |
| url | https://peerj.com/articles/101.pdf |
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