Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran
Introduction: Diffusion of antibiotic resistance genes by horizontal gene transfer has led to the fast emergence of multidrug resistance (MDR) among bacteria. Multiple classes of integrons are effective genetic elements which play a significant role in the acquisition and nosocomial dissemination of...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2018-10-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | http://jommid.pasteur.ac.ir/article-1-186-en.html |
| _version_ | 1818871308540182528 |
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| author | Mojtaba Moosavian Mahtab Khoshkholgh Sima Maryam Haddadzadeh Shoushtari Mohammad amin Fazeli Naserabad |
| author_facet | Mojtaba Moosavian Mahtab Khoshkholgh Sima Maryam Haddadzadeh Shoushtari Mohammad amin Fazeli Naserabad |
| author_sort | Mojtaba Moosavian |
| collection | DOAJ |
| description | Introduction: Diffusion of antibiotic resistance genes by horizontal gene transfer has led to the fast emergence of multidrug resistance (MDR) among bacteria. Multiple classes of integrons are effective genetic elements which play a significant role in the acquisition and nosocomial dissemination of resistance factors in strains of Gram-negative bacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. Methods: In this study, 110 sputum samples were collected from hospitalized patients with tract infections. Identification of the isolates was performed by standard biochemical tests. The most frequent Gram-negative isolates were 25 Enterobacteriaceae (62.5%), (9 Enterobacter spp, 11 Citrobacter spp, and 5 Escherichia coli), 6 P. aeruginosa (15%) and 9 Acinetobacter spp (22.5%). Susceptibility of the isolates to antibiotics was carried out by Kirby-Bauer disk diffusion method according to CLSI guidelines, and finally, the class 1 integrons were detected by PCR. Results: Maximum resistance rate among Gram-negative isolates was observed to ceftazidime, co-trimoxazole, and cefotaxime with 89%, 87%, and 82%, respectively. A low-level resistance was recognized for imipenem 32% and gentamicin 34%, while an intermediate level resistance was found against the norfloxacin 40% and ciprofloxacin 44%. Out of 6 P. aeruginosa and 9 A. baumannii isolates, 2 (33.3%) and 3 isolates (33.3%) were positive for class 1 Integrons, respectively, while all Enterobacteriaceae isolates (100%) were negative for class 1 Integrons. Class 1 integrons were detected among of MDR isolates. Conclusion: Our results showed that monitoring MDR isolates and detection of class 1 integrons in these isolates is necessary for promotion of antibacterial stewardship. |
| first_indexed | 2024-12-19T12:20:51Z |
| format | Article |
| id | doaj.art-ba92fed4190b4e68b3e353e33e7915f5 |
| institution | Directory Open Access Journal |
| issn | 2345-5349 2345-5330 |
| language | English |
| last_indexed | 2024-12-19T12:20:51Z |
| publishDate | 2018-10-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj.art-ba92fed4190b4e68b3e353e33e7915f52022-12-21T20:21:46ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302018-10-0164103107Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, IranMojtaba Moosavian0Mahtab Khoshkholgh Sima1Maryam Haddadzadeh Shoushtari2Mohammad amin Fazeli Naserabad3 Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Pulmonology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Razi Teaching Hospital, Clinical Research Development Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Introduction: Diffusion of antibiotic resistance genes by horizontal gene transfer has led to the fast emergence of multidrug resistance (MDR) among bacteria. Multiple classes of integrons are effective genetic elements which play a significant role in the acquisition and nosocomial dissemination of resistance factors in strains of Gram-negative bacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. Methods: In this study, 110 sputum samples were collected from hospitalized patients with tract infections. Identification of the isolates was performed by standard biochemical tests. The most frequent Gram-negative isolates were 25 Enterobacteriaceae (62.5%), (9 Enterobacter spp, 11 Citrobacter spp, and 5 Escherichia coli), 6 P. aeruginosa (15%) and 9 Acinetobacter spp (22.5%). Susceptibility of the isolates to antibiotics was carried out by Kirby-Bauer disk diffusion method according to CLSI guidelines, and finally, the class 1 integrons were detected by PCR. Results: Maximum resistance rate among Gram-negative isolates was observed to ceftazidime, co-trimoxazole, and cefotaxime with 89%, 87%, and 82%, respectively. A low-level resistance was recognized for imipenem 32% and gentamicin 34%, while an intermediate level resistance was found against the norfloxacin 40% and ciprofloxacin 44%. Out of 6 P. aeruginosa and 9 A. baumannii isolates, 2 (33.3%) and 3 isolates (33.3%) were positive for class 1 Integrons, respectively, while all Enterobacteriaceae isolates (100%) were negative for class 1 Integrons. Class 1 integrons were detected among of MDR isolates. Conclusion: Our results showed that monitoring MDR isolates and detection of class 1 integrons in these isolates is necessary for promotion of antibacterial stewardship.http://jommid.pasteur.ac.ir/article-1-186-en.htmlclass 1 integronsmultidrug resistancepseudomonas aeruginosaacinetobacter baumanniienterobacteriaceae. |
| spellingShingle | Mojtaba Moosavian Mahtab Khoshkholgh Sima Maryam Haddadzadeh Shoushtari Mohammad amin Fazeli Naserabad Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran Journal of Medical Microbiology and Infectious Diseases class 1 integrons multidrug resistance pseudomonas aeruginosa acinetobacter baumannii enterobacteriaceae. |
| title | Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran |
| title_full | Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran |
| title_fullStr | Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran |
| title_full_unstemmed | Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran |
| title_short | Detection of Class 1 Integrons among Gram-negative Bacilli Isolated from Sputum Cultures of Patients with Lower Respiratory Tract Infections in Ahvaz, Iran |
| title_sort | detection of class 1 integrons among gram negative bacilli isolated from sputum cultures of patients with lower respiratory tract infections in ahvaz iran |
| topic | class 1 integrons multidrug resistance pseudomonas aeruginosa acinetobacter baumannii enterobacteriaceae. |
| url | http://jommid.pasteur.ac.ir/article-1-186-en.html |
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