Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2
This study focuses on the phenol biodegradation kinetics by <i>Stenotrophomonas maltophilia</i> KB2 in a nickel-contaminated medium. Initial tests proved that a nickel concentration of 33.3 mg·L<sup>−1</sup> caused a cessation of bacterial growth. The experiments were conduct...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/14/20/6058 |
| _version_ | 1797514060235800576 |
|---|---|
| author | Agnieszka Gąszczak Elżbieta Szczyrba Anna Szczotka Izabela Greń |
| author_facet | Agnieszka Gąszczak Elżbieta Szczyrba Anna Szczotka Izabela Greń |
| author_sort | Agnieszka Gąszczak |
| collection | DOAJ |
| description | This study focuses on the phenol biodegradation kinetics by <i>Stenotrophomonas maltophilia</i> KB2 in a nickel-contaminated medium. Initial tests proved that a nickel concentration of 33.3 mg·L<sup>−1</sup> caused a cessation of bacterial growth. The experiments were conducted in a batch bioreactor in several series: without nickel, at constant nickel concentration and at varying metal concentrations (1.67–13.33 g·m<sup>−3</sup>). For a constant Ni<sup>2+</sup> concentration (1.67 or 3.33 g·m<sup>−3</sup>), a comparable bacterial growth rate was obtained regardless of the initial phenol concentration (50–300 g·m<sup>−3</sup>). The dependence <i>µ</i> = f (<i>S</i><sub>0</sub>) at constant Ni<sup>2+</sup> concentration was very well described by the Monod equations. The created varying nickel concentrations experimental database was used to estimate the parameters of selected mathematical models, and the analysis included different methods of determining metal inhibition constant <i>K<sub>IM</sub></i>. Each model showed a very good fit with the experimental data (R<sup>2</sup> values were higher than 0.9). The best agreement (R<sup>2</sup> = 0.995) was achieved using a modified Andrews equation, which considers the metal influence and substrate inhibition. Therefore, kinetic equation parameters were estimated: <i>µ<sub>max</sub></i> = 1.584 h<sup>−1</sup>, <i>K<sub>S</sub></i> = 185.367 g·m<sup>−3</sup>, <i>K<sub>IS</sub></i> = 106.137 g·m<sup>−3</sup>, <i>K<sub>IM</sub></i> = 1.249 g·m<sup>−3</sup> and <i>n</i> = 1.0706. |
| first_indexed | 2024-03-10T06:26:17Z |
| format | Article |
| id | doaj.art-ba93f90d70bb42f6acb2c9c49457123c |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T06:26:17Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-ba93f90d70bb42f6acb2c9c49457123c2023-11-22T18:58:21ZengMDPI AGMaterials1996-19442021-10-011420605810.3390/ma14206058Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2Agnieszka Gąszczak0Elżbieta Szczyrba1Anna Szczotka2Izabela Greń3Institute of Chemical Engineering, Polish Academy of Sciences, ul. Bałtycka 5, 44-100 Gliwice, PolandInstitute of Chemical Engineering, Polish Academy of Sciences, ul. Bałtycka 5, 44-100 Gliwice, PolandInstitute of Chemical Engineering, Polish Academy of Sciences, ul. Bałtycka 5, 44-100 Gliwice, PolandFaculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, ul. Jagiellońska 28, 40-032 Katowice, PolandThis study focuses on the phenol biodegradation kinetics by <i>Stenotrophomonas maltophilia</i> KB2 in a nickel-contaminated medium. Initial tests proved that a nickel concentration of 33.3 mg·L<sup>−1</sup> caused a cessation of bacterial growth. The experiments were conducted in a batch bioreactor in several series: without nickel, at constant nickel concentration and at varying metal concentrations (1.67–13.33 g·m<sup>−3</sup>). For a constant Ni<sup>2+</sup> concentration (1.67 or 3.33 g·m<sup>−3</sup>), a comparable bacterial growth rate was obtained regardless of the initial phenol concentration (50–300 g·m<sup>−3</sup>). The dependence <i>µ</i> = f (<i>S</i><sub>0</sub>) at constant Ni<sup>2+</sup> concentration was very well described by the Monod equations. The created varying nickel concentrations experimental database was used to estimate the parameters of selected mathematical models, and the analysis included different methods of determining metal inhibition constant <i>K<sub>IM</sub></i>. Each model showed a very good fit with the experimental data (R<sup>2</sup> values were higher than 0.9). The best agreement (R<sup>2</sup> = 0.995) was achieved using a modified Andrews equation, which considers the metal influence and substrate inhibition. Therefore, kinetic equation parameters were estimated: <i>µ<sub>max</sub></i> = 1.584 h<sup>−1</sup>, <i>K<sub>S</sub></i> = 185.367 g·m<sup>−3</sup>, <i>K<sub>IS</sub></i> = 106.137 g·m<sup>−3</sup>, <i>K<sub>IM</sub></i> = 1.249 g·m<sup>−3</sup> and <i>n</i> = 1.0706.https://www.mdpi.com/1996-1944/14/20/6058heavy metal inhibitionkinetic equationsnickelphenol biodegradation |
| spellingShingle | Agnieszka Gąszczak Elżbieta Szczyrba Anna Szczotka Izabela Greń Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2 Materials heavy metal inhibition kinetic equations nickel phenol biodegradation |
| title | Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2 |
| title_full | Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2 |
| title_fullStr | Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2 |
| title_full_unstemmed | Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2 |
| title_short | Effect of Nickel as Stress Factor on Phenol Biodegradation by <i>Stenotrophomonas maltophilia</i> KB2 |
| title_sort | effect of nickel as stress factor on phenol biodegradation by i stenotrophomonas maltophilia i kb2 |
| topic | heavy metal inhibition kinetic equations nickel phenol biodegradation |
| url | https://www.mdpi.com/1996-1944/14/20/6058 |
| work_keys_str_mv | AT agnieszkagaszczak effectofnickelasstressfactoronphenolbiodegradationbyistenotrophomonasmaltophiliaikb2 AT elzbietaszczyrba effectofnickelasstressfactoronphenolbiodegradationbyistenotrophomonasmaltophiliaikb2 AT annaszczotka effectofnickelasstressfactoronphenolbiodegradationbyistenotrophomonasmaltophiliaikb2 AT izabelagren effectofnickelasstressfactoronphenolbiodegradationbyistenotrophomonasmaltophiliaikb2 |