Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity
Here, we sought to monitor bone marrow–derived dendritic cell (BMDC) migration and antitumor effects using a multimodal reporter imaging strategy in living mice. BMDCs were transduced with retroviral vector harboring human sodium iodide symporter (hNIS, nuclear imaging reporter), firefly luc2 (optic...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-04-01
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| Series: | Translational Oncology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S193652331630225X |
| _version_ | 1819267372910903296 |
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| author | Su-Bi Ahn Sang Bong Lee Thoudam Debraj Singh Sung Jin Cho Sang Kyoon Kim In-Kyu Lee Shin Young Jeong Byeong-Cheol Ahn Jaetae Lee Sang-Woo Lee Yong Hyun Jeon |
| author_facet | Su-Bi Ahn Sang Bong Lee Thoudam Debraj Singh Sung Jin Cho Sang Kyoon Kim In-Kyu Lee Shin Young Jeong Byeong-Cheol Ahn Jaetae Lee Sang-Woo Lee Yong Hyun Jeon |
| author_sort | Su-Bi Ahn |
| collection | DOAJ |
| description | Here, we sought to monitor bone marrow–derived dendritic cell (BMDC) migration and antitumor effects using a multimodal reporter imaging strategy in living mice. BMDCs were transduced with retroviral vector harboring human sodium iodide symporter (hNIS, nuclear imaging reporter), firefly luc2 (optical imaging reporter), and thy1.1 (surrogate marker of NIS and luc2) genes (BMDC/NF cells). No significant differences in biological functions, including cell proliferation, antigen uptake, phenotype expression, and migration ability, were observed between BMDC and BMDC/NF cells. Combined bioluminescence imaging and I-124 positron emission tomography/computed tomography clearly revealed the migration of BMDC/NF cells to draining popliteal lymph nodes at day 7 postinjection. Interestingly, marked tumor protection was observed in mice immunized with TC-1 lysate-pulsed BMDC/NF cells. Our findings suggested that multimodal reporter gene imaging of NIS and luciferase could provide insights into the biological behaviors of dendritic cells in living organisms and could be a useful tool for the optimization of DC-based immunotherapy protocols. |
| first_indexed | 2024-12-23T21:16:08Z |
| format | Article |
| id | doaj.art-ba948fab6f6d42d881492c82ea157ec3 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 1944-7124 |
| language | English |
| last_indexed | 2024-12-23T21:16:08Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-ba948fab6f6d42d881492c82ea157ec32022-12-21T17:30:54ZengElsevierTranslational Oncology1936-52331944-71242017-04-0110226227010.1016/j.tranon.2017.01.003Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor ImmunitySu-Bi Ahn0Sang Bong Lee1Thoudam Debraj Singh2Sung Jin Cho3Sang Kyoon Kim4In-Kyu Lee5Shin Young Jeong6Byeong-Cheol Ahn7Jaetae Lee8Sang-Woo Lee9Yong Hyun Jeon10Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaLeading-edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaLeading-edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaDepartment of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South KoreaLeading-edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu, South KoreaHere, we sought to monitor bone marrow–derived dendritic cell (BMDC) migration and antitumor effects using a multimodal reporter imaging strategy in living mice. BMDCs were transduced with retroviral vector harboring human sodium iodide symporter (hNIS, nuclear imaging reporter), firefly luc2 (optical imaging reporter), and thy1.1 (surrogate marker of NIS and luc2) genes (BMDC/NF cells). No significant differences in biological functions, including cell proliferation, antigen uptake, phenotype expression, and migration ability, were observed between BMDC and BMDC/NF cells. Combined bioluminescence imaging and I-124 positron emission tomography/computed tomography clearly revealed the migration of BMDC/NF cells to draining popliteal lymph nodes at day 7 postinjection. Interestingly, marked tumor protection was observed in mice immunized with TC-1 lysate-pulsed BMDC/NF cells. Our findings suggested that multimodal reporter gene imaging of NIS and luciferase could provide insights into the biological behaviors of dendritic cells in living organisms and could be a useful tool for the optimization of DC-based immunotherapy protocols.http://www.sciencedirect.com/science/article/pii/S193652331630225X |
| spellingShingle | Su-Bi Ahn Sang Bong Lee Thoudam Debraj Singh Sung Jin Cho Sang Kyoon Kim In-Kyu Lee Shin Young Jeong Byeong-Cheol Ahn Jaetae Lee Sang-Woo Lee Yong Hyun Jeon Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity Translational Oncology |
| title | Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity |
| title_full | Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity |
| title_fullStr | Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity |
| title_full_unstemmed | Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity |
| title_short | Multimodality Imaging of Bone Marrow–Derived Dendritic Cell Migration and Antitumor Immunity |
| title_sort | multimodality imaging of bone marrow derived dendritic cell migration and antitumor immunity |
| url | http://www.sciencedirect.com/science/article/pii/S193652331630225X |
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