Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces
Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2018-07-01
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| Series: | Materials Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392018000500223&tlng=en |
| _version_ | 1798021664069386240 |
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| author | Leonardo Marasca Antonini Adilar Gonçalves dos Santos Junior Gwendolen Reilly Célia de Fraga Malfatti |
| author_facet | Leonardo Marasca Antonini Adilar Gonçalves dos Santos Junior Gwendolen Reilly Célia de Fraga Malfatti |
| author_sort | Leonardo Marasca Antonini |
| collection | DOAJ |
| description | Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface. |
| first_indexed | 2024-04-11T17:17:14Z |
| format | Article |
| id | doaj.art-ba95f44e5eae4f76960831fd18e1ac83 |
| institution | Directory Open Access Journal |
| issn | 1516-1439 |
| language | English |
| last_indexed | 2024-04-11T17:17:14Z |
| publishDate | 2018-07-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj.art-ba95f44e5eae4f76960831fd18e1ac832022-12-22T04:12:35ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392018-07-0121510.1590/1980-5373-mr-2017-1081Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V SurfacesLeonardo Marasca Antoninihttps://orcid.org/0000-0002-4961-3436Adilar Gonçalves dos Santos JuniorGwendolen ReillyCélia de Fraga MalfattiNanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392018000500223&tlng=enhESCs-MP cellsTi6Al4Velectropolishingnanometric and micrometric roughnesswettability |
| spellingShingle | Leonardo Marasca Antonini Adilar Gonçalves dos Santos Junior Gwendolen Reilly Célia de Fraga Malfatti Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces Materials Research hESCs-MP cells Ti6Al4V electropolishing nanometric and micrometric roughness wettability |
| title | Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces |
| title_full | Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces |
| title_fullStr | Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces |
| title_full_unstemmed | Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces |
| title_short | Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces |
| title_sort | human embryonic stem cell derived mesenchymal progenitor hescs mp growth on nanostructured ti6al4v surfaces |
| topic | hESCs-MP cells Ti6Al4V electropolishing nanometric and micrometric roughness wettability |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392018000500223&tlng=en |
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