Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i>
Ferulic acid (FA) is a naturally-occurring well-known potent antioxidant and free radical scavenger. FA supplementation is an effective strategy to delay aging, but the underlying mechanism remains unknown. In the present study, we examined the effects of FA on lifespan extension and its mechanism o...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/8/4279 |
| _version_ | 1797537007924150272 |
|---|---|
| author | Hui Li Xiaoxuan Yu Fanwei Meng Zhenyu Zhao Shuwen Guan Liping Wang |
| author_facet | Hui Li Xiaoxuan Yu Fanwei Meng Zhenyu Zhao Shuwen Guan Liping Wang |
| author_sort | Hui Li |
| collection | DOAJ |
| description | Ferulic acid (FA) is a naturally-occurring well-known potent antioxidant and free radical scavenger. FA supplementation is an effective strategy to delay aging, but the underlying mechanism remains unknown. In the present study, we examined the effects of FA on lifespan extension and its mechanism of FA in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Results suggested that FA increased the lifespan of <i>C. elegans</i>, rather than altering the growth of <i>E. coli</i> OP50. Meanwhile, FA promoted the healthspan of <i>C. elegans</i> by improving locomotion and reducing fat accumulation and polyQ aggregation. FA increased the resistance to heat and oxidative stress through reducing ROS. The upregulating of the expression of the <i>hlh-30</i>, <i>skn-1</i>, and <i>hsf-1</i> were involved in the FA-mediated lifespan extension. Furthermore, FA treatment had no impact on the lifespan of <i>daf-2</i>, <i>hlh-30</i>, <i>skn-1</i>, and <i>hsf-1</i> mutants, confirming that insulin/IGF-1 signaling pathway and multiple longevity mechanisms were associated with the longevity mechanism of FA. We further found that mitochondrial signaling pathway was modulation involved in FA-mediated lifespan extension. With the results from RNA-seq results and mutants lifespan assay. These findings contribute to our knowledge of the lifespan extension and underlying mechanism of action of FA in <i>C. elegans</i>. |
| first_indexed | 2024-03-10T12:08:58Z |
| format | Article |
| id | doaj.art-ba963b2f102940db87651c59cf7fe17a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:08:58Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ba963b2f102940db87651c59cf7fe17a2023-11-21T16:21:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228427910.3390/ijms22084279Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i>Hui Li0Xiaoxuan Yu1Fanwei Meng2Zhenyu Zhao3Shuwen Guan4Liping Wang5Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Changchun 130012, ChinaFerulic acid (FA) is a naturally-occurring well-known potent antioxidant and free radical scavenger. FA supplementation is an effective strategy to delay aging, but the underlying mechanism remains unknown. In the present study, we examined the effects of FA on lifespan extension and its mechanism of FA in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). Results suggested that FA increased the lifespan of <i>C. elegans</i>, rather than altering the growth of <i>E. coli</i> OP50. Meanwhile, FA promoted the healthspan of <i>C. elegans</i> by improving locomotion and reducing fat accumulation and polyQ aggregation. FA increased the resistance to heat and oxidative stress through reducing ROS. The upregulating of the expression of the <i>hlh-30</i>, <i>skn-1</i>, and <i>hsf-1</i> were involved in the FA-mediated lifespan extension. Furthermore, FA treatment had no impact on the lifespan of <i>daf-2</i>, <i>hlh-30</i>, <i>skn-1</i>, and <i>hsf-1</i> mutants, confirming that insulin/IGF-1 signaling pathway and multiple longevity mechanisms were associated with the longevity mechanism of FA. We further found that mitochondrial signaling pathway was modulation involved in FA-mediated lifespan extension. With the results from RNA-seq results and mutants lifespan assay. These findings contribute to our knowledge of the lifespan extension and underlying mechanism of action of FA in <i>C. elegans</i>.https://www.mdpi.com/1422-0067/22/8/4279ferulic acidlifespanstress resistantinsulin/IGF-1<i>Caenorhabditis elegans</i> |
| spellingShingle | Hui Li Xiaoxuan Yu Fanwei Meng Zhenyu Zhao Shuwen Guan Liping Wang Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i> International Journal of Molecular Sciences ferulic acid lifespan stress resistant insulin/IGF-1 <i>Caenorhabditis elegans</i> |
| title | Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i> |
| title_full | Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i> |
| title_fullStr | Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i> |
| title_full_unstemmed | Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i> |
| title_short | Ferulic Acid Supplementation Increases Lifespan and Stress Resistance via Insulin/IGF-1 Signaling Pathway in <i>C. elegans</i> |
| title_sort | ferulic acid supplementation increases lifespan and stress resistance via insulin igf 1 signaling pathway in i c elegans i |
| topic | ferulic acid lifespan stress resistant insulin/IGF-1 <i>Caenorhabditis elegans</i> |
| url | https://www.mdpi.com/1422-0067/22/8/4279 |
| work_keys_str_mv | AT huili ferulicacidsupplementationincreaseslifespanandstressresistanceviainsulinigf1signalingpathwayinicelegansi AT xiaoxuanyu ferulicacidsupplementationincreaseslifespanandstressresistanceviainsulinigf1signalingpathwayinicelegansi AT fanweimeng ferulicacidsupplementationincreaseslifespanandstressresistanceviainsulinigf1signalingpathwayinicelegansi AT zhenyuzhao ferulicacidsupplementationincreaseslifespanandstressresistanceviainsulinigf1signalingpathwayinicelegansi AT shuwenguan ferulicacidsupplementationincreaseslifespanandstressresistanceviainsulinigf1signalingpathwayinicelegansi AT lipingwang ferulicacidsupplementationincreaseslifespanandstressresistanceviainsulinigf1signalingpathwayinicelegansi |