HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity
<i>Pseudomonas syringae</i>-secreted HopA1 effectors are important determinants in host range expansion and increased pathogenicity. Their recent acquisitions via horizontal gene transfer in several non-pathogenic Pseudomonas strains worldwide have caused alarming increase in their virul...
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2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Shraddha K. Dahale Daipayan Ghosh Kishor D. Ingole Anup Chugani Sang Hee Kim Saikat Bhattacharjee |
| author_facet | Shraddha K. Dahale Daipayan Ghosh Kishor D. Ingole Anup Chugani Sang Hee Kim Saikat Bhattacharjee |
| author_sort | Shraddha K. Dahale |
| collection | DOAJ |
| description | <i>Pseudomonas syringae</i>-secreted HopA1 effectors are important determinants in host range expansion and increased pathogenicity. Their recent acquisitions via horizontal gene transfer in several non-pathogenic Pseudomonas strains worldwide have caused alarming increase in their virulence capabilities. In <i>Arabidopsis thaliana</i>, <i>RESISTANCE TO PSEUDOMONAS SYRINGAE 6</i> (<i>RPS6</i>) gene confers effector-triggered immunity (ETI) against HopA1<sub>pss</sub> derived from <i>P. syringae</i> pv. <i>syringae</i> strain 61. Surprisingly, a closely related HopA1<sub>pst</sub> from the tomato pathovar evades immune detection. These responsive differences <i>in planta</i> between the two HopA1s represents a unique system to study pathogen adaptation skills and host-jumps. However, molecular understanding of HopA1′s contribution to overall virulence remain undeciphered. Here, we show that immune-suppressive functions of HopA1<sub>pst</sub> are more potent than HopA1<sub>pss</sub>. In the resistance-compromised <i>ENHANCED DISEASE SUSCEPTIBILITY 1</i> (<i>EDS1</i>) null-mutant, transcriptomic changes associated with HopA1<sub>pss</sub>-elicited ETI are still induced and carry resemblance to PAMP-triggered immunity (PTI) signatures. Enrichment of HopA1<sub>pss</sub> interactome identifies proteins with regulatory roles in post-transcriptional and translational processes. With our demonstration here that both HopA1 suppress reporter-gene translations in vitro imply that the above effector-associations with plant target carry inhibitory consequences. Overall, with our results here we unravel possible virulence role(s) of HopA1 in suppressing PTI and provide newer insights into its detection in resistant plants. |
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| language | English |
| last_indexed | 2024-03-10T09:37:34Z |
| publishDate | 2021-07-01 |
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| spelling | doaj.art-ba96c9563e3a42768573ee9785c3e5862023-11-22T03:58:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214744010.3390/ijms22147440HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered ImmunityShraddha K. Dahale0Daipayan Ghosh1Kishor D. Ingole2Anup Chugani3Sang Hee Kim4Saikat Bhattacharjee5Laboratory of Signal Transduction and Plant Resistance, UNESCO-Regional Centre for Biotechnology (RCB), NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121 001, Haryana, IndiaLaboratory of Signal Transduction and Plant Resistance, UNESCO-Regional Centre for Biotechnology (RCB), NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121 001, Haryana, IndiaLaboratory of Signal Transduction and Plant Resistance, UNESCO-Regional Centre for Biotechnology (RCB), NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121 001, Haryana, IndiaMedGenome Labs Ltd., 3rd Floor, Narayana Nethralaya Building, Narayana Health City, 258/A, Bommasandra, Hosur Road, Bengaluru 560099, Karnataka, IndiaDivision of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Division of Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju 52828, KoreaLaboratory of Signal Transduction and Plant Resistance, UNESCO-Regional Centre for Biotechnology (RCB), NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121 001, Haryana, India<i>Pseudomonas syringae</i>-secreted HopA1 effectors are important determinants in host range expansion and increased pathogenicity. Their recent acquisitions via horizontal gene transfer in several non-pathogenic Pseudomonas strains worldwide have caused alarming increase in their virulence capabilities. In <i>Arabidopsis thaliana</i>, <i>RESISTANCE TO PSEUDOMONAS SYRINGAE 6</i> (<i>RPS6</i>) gene confers effector-triggered immunity (ETI) against HopA1<sub>pss</sub> derived from <i>P. syringae</i> pv. <i>syringae</i> strain 61. Surprisingly, a closely related HopA1<sub>pst</sub> from the tomato pathovar evades immune detection. These responsive differences <i>in planta</i> between the two HopA1s represents a unique system to study pathogen adaptation skills and host-jumps. However, molecular understanding of HopA1′s contribution to overall virulence remain undeciphered. Here, we show that immune-suppressive functions of HopA1<sub>pst</sub> are more potent than HopA1<sub>pss</sub>. In the resistance-compromised <i>ENHANCED DISEASE SUSCEPTIBILITY 1</i> (<i>EDS1</i>) null-mutant, transcriptomic changes associated with HopA1<sub>pss</sub>-elicited ETI are still induced and carry resemblance to PAMP-triggered immunity (PTI) signatures. Enrichment of HopA1<sub>pss</sub> interactome identifies proteins with regulatory roles in post-transcriptional and translational processes. With our demonstration here that both HopA1 suppress reporter-gene translations in vitro imply that the above effector-associations with plant target carry inhibitory consequences. Overall, with our results here we unravel possible virulence role(s) of HopA1 in suppressing PTI and provide newer insights into its detection in resistant plants.https://www.mdpi.com/1422-0067/22/14/7440effectorsvirulencePAMP-triggered immunityeffector-triggered immunityalternative splicingnonsense-mediated decay |
| spellingShingle | Shraddha K. Dahale Daipayan Ghosh Kishor D. Ingole Anup Chugani Sang Hee Kim Saikat Bhattacharjee HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity International Journal of Molecular Sciences effectors virulence PAMP-triggered immunity effector-triggered immunity alternative splicing nonsense-mediated decay |
| title | HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity |
| title_full | HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity |
| title_fullStr | HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity |
| title_full_unstemmed | HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity |
| title_short | HopA1 Effector from <i>Pseudomonas syringae</i> pv <i>syringae</i> Strain 61 Affects NMD Processes and Elicits Effector-Triggered Immunity |
| title_sort | hopa1 effector from i pseudomonas syringae i pv i syringae i strain 61 affects nmd processes and elicits effector triggered immunity |
| topic | effectors virulence PAMP-triggered immunity effector-triggered immunity alternative splicing nonsense-mediated decay |
| url | https://www.mdpi.com/1422-0067/22/14/7440 |
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