Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content
<i>Hedera helix</i> L. is known for its therapeutic properties, such as analgesic, anti-inflammatory, expectorant activity. It is currently known that the characteristic therapeutic effects of ivy extracts are induced by phytocompunds, such as: saponins (hederagenin, α and β-hederin, hed...
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MDPI AG
2022-03-01
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| author | Rodica Tatia Lucia Moldovan Isabela Tarcomnicu Anca Daniela Raiciu Adina Gavrila Ioan Calinescu Christina Zalaru |
| author_facet | Rodica Tatia Lucia Moldovan Isabela Tarcomnicu Anca Daniela Raiciu Adina Gavrila Ioan Calinescu Christina Zalaru |
| author_sort | Rodica Tatia |
| collection | DOAJ |
| description | <i>Hedera helix</i> L. is known for its therapeutic properties, such as analgesic, anti-inflammatory, expectorant activity. It is currently known that the characteristic therapeutic effects of ivy extracts are induced by phytocompunds, such as: saponins (hederagenin, α and β-hederin, hederacoside B and C), phytosterols (sitosterol, stigmasterol, campesterol), flavonoids, falcarinol, falcarinone, scopoline, chlorogenic acid, caffeic acid, phytoestrogens [<sup>1</sup>]. The purpose of our study was to evaluate the total saponin content of <i>Hedera helix</i> L. leaves extracts obtained by both conventional, and unconventional methods. The commercial fresh leaves of <i>Hedera helix</i> L. were purchased from Hofigal SA, Romania. The following reagents used for testing were α-hederin, hederagenin, and hederacoside C at purity ≥98% (HPLC), DMSO were purchased from Sigma Aldrich. The chemical composition of the obtained extracts was analyzed by HPLC-MS/MS, and the total saponin content was evaluated [<sup>2</sup>,<sup>3</sup>]. Our study indicated an optimal method for obtaining <i>Hedera helix</i> L. leaves extract with an enriched saponin content. |
| first_indexed | 2024-03-11T02:38:12Z |
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| issn | 2673-4583 |
| language | English |
| last_indexed | 2024-03-11T02:38:12Z |
| publishDate | 2022-03-01 |
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| spelling | doaj.art-ba99a843060d431f9905740530ab4f792023-11-18T09:47:10ZengMDPI AGChemistry Proceedings2673-45832022-03-01715610.3390/chemproc2022007056Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins ContentRodica Tatia0Lucia Moldovan1Isabela Tarcomnicu2Anca Daniela Raiciu3Adina Gavrila4Ioan Calinescu5Christina Zalaru6Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independenţei, 060031 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independenţei, 060031 Bucharest, RomaniaCytogenomic Medical Laboratory, 35 Floreasca Street, 014462 Bucharest, RomaniaHofigal Export-Import SRL, 2 Greenhouse Entrance Street, 042124 Bucharest, RomaniaDepartment of Bioresources and Polymer Science, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaDepartment of Bioresources and Polymer Science, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaDepartment of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Street, 050663 Bucharest, Romania<i>Hedera helix</i> L. is known for its therapeutic properties, such as analgesic, anti-inflammatory, expectorant activity. It is currently known that the characteristic therapeutic effects of ivy extracts are induced by phytocompunds, such as: saponins (hederagenin, α and β-hederin, hederacoside B and C), phytosterols (sitosterol, stigmasterol, campesterol), flavonoids, falcarinol, falcarinone, scopoline, chlorogenic acid, caffeic acid, phytoestrogens [<sup>1</sup>]. The purpose of our study was to evaluate the total saponin content of <i>Hedera helix</i> L. leaves extracts obtained by both conventional, and unconventional methods. The commercial fresh leaves of <i>Hedera helix</i> L. were purchased from Hofigal SA, Romania. The following reagents used for testing were α-hederin, hederagenin, and hederacoside C at purity ≥98% (HPLC), DMSO were purchased from Sigma Aldrich. The chemical composition of the obtained extracts was analyzed by HPLC-MS/MS, and the total saponin content was evaluated [<sup>2</sup>,<sup>3</sup>]. Our study indicated an optimal method for obtaining <i>Hedera helix</i> L. leaves extract with an enriched saponin content.https://www.mdpi.com/2673-4583/7/1/56<i>Hedera helix</i> L. leaves extractHPLC-MS/MStotal saponin content |
| spellingShingle | Rodica Tatia Lucia Moldovan Isabela Tarcomnicu Anca Daniela Raiciu Adina Gavrila Ioan Calinescu Christina Zalaru Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content Chemistry Proceedings <i>Hedera helix</i> L. leaves extract HPLC-MS/MS total saponin content |
| title | Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content |
| title_full | Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content |
| title_fullStr | Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content |
| title_full_unstemmed | Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content |
| title_short | Methods of Obtaining Extracts from <i>Hedera helix</i> L. Leaves and Evaluation of the Total Saponins Content |
| title_sort | methods of obtaining extracts from i hedera helix i l leaves and evaluation of the total saponins content |
| topic | <i>Hedera helix</i> L. leaves extract HPLC-MS/MS total saponin content |
| url | https://www.mdpi.com/2673-4583/7/1/56 |
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