A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform.
Subtle alternative splicing leads to the formation of RNA variants lacking or including a small number of nucleotides. To date, the impact of subtle alternative splicing phenomena on protein biosynthesis has been studied in frame-preserving incidents. On the contrary, mRNA isoforms derived from fram...
Main Authors: | , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4010519?pdf=render |
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author | Evangelos D Karousis Diamantis C Sideris |
author_facet | Evangelos D Karousis Diamantis C Sideris |
author_sort | Evangelos D Karousis |
collection | DOAJ |
description | Subtle alternative splicing leads to the formation of RNA variants lacking or including a small number of nucleotides. To date, the impact of subtle alternative splicing phenomena on protein biosynthesis has been studied in frame-preserving incidents. On the contrary, mRNA isoforms derived from frame-shifting events were poorly studied and generally characterized as non-coding. This work provides evidence for a frame-shifting subtle alternative splicing event which results in the production of a novel protein isoform. We applied a combined molecular approach for the cloning and expression analysis of a human RNase κ transcript (RNase κ-02) which lacks four consecutive bases compared to the previously isolated RNase κ isoform. RNase κ-02 mRNA is expressed in all human cell lines tested end encodes the synthesis of a 134-amino-acid protein by utilizing an alternative initiation codon. The expression of RNase κ-02 in the cytoplasm of human cells was verified by Western blot and immunofluorescence analysis using a specific polyclonal antibody developed on the basis of the amino-acid sequence difference between the two protein isoforms. The results presented here show that subtle changes during mRNA splicing can lead to the expression of significantly altered protein isoforms. |
first_indexed | 2024-04-13T13:06:15Z |
format | Article |
id | doaj.art-ba9a04c1ce5a4e34b2782bb1352653c4 |
institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-04-13T13:06:15Z |
publishDate | 2014-01-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS ONE |
spelling | doaj.art-ba9a04c1ce5a4e34b2782bb1352653c42022-12-22T02:45:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9655710.1371/journal.pone.0096557A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform.Evangelos D KarousisDiamantis C SiderisSubtle alternative splicing leads to the formation of RNA variants lacking or including a small number of nucleotides. To date, the impact of subtle alternative splicing phenomena on protein biosynthesis has been studied in frame-preserving incidents. On the contrary, mRNA isoforms derived from frame-shifting events were poorly studied and generally characterized as non-coding. This work provides evidence for a frame-shifting subtle alternative splicing event which results in the production of a novel protein isoform. We applied a combined molecular approach for the cloning and expression analysis of a human RNase κ transcript (RNase κ-02) which lacks four consecutive bases compared to the previously isolated RNase κ isoform. RNase κ-02 mRNA is expressed in all human cell lines tested end encodes the synthesis of a 134-amino-acid protein by utilizing an alternative initiation codon. The expression of RNase κ-02 in the cytoplasm of human cells was verified by Western blot and immunofluorescence analysis using a specific polyclonal antibody developed on the basis of the amino-acid sequence difference between the two protein isoforms. The results presented here show that subtle changes during mRNA splicing can lead to the expression of significantly altered protein isoforms.http://europepmc.org/articles/PMC4010519?pdf=render |
spellingShingle | Evangelos D Karousis Diamantis C Sideris A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform. PLoS ONE |
title | A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform. |
title_full | A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform. |
title_fullStr | A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform. |
title_full_unstemmed | A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform. |
title_short | A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform. |
title_sort | subtle alternative splicing event gives rise to a widely expressed human rnase k isoform |
url | http://europepmc.org/articles/PMC4010519?pdf=render |
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