Growth Time of Candida Species in Blood Culture Bottles and Approaches to Improve This Time
Introduction: Hospital-acquired Candida infections are difficult to diagnose and treat, and are costly infectious diseases. Mortality rates are very high when compared to those of other infectious diseases. Antifungal therapy should be initiated without delay. Therefore, it is important to develop a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2022-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-02/241-248%20Elif%20Ayca%20Sahin.pdf |
| Summary: | Introduction: Hospital-acquired Candida infections are difficult to diagnose and treat, and are costly infectious diseases. Mortality rates are very high when compared to those of other infectious diseases. Antifungal therapy should be initiated without delay. Therefore, it is important to develop an approach that accelerates the fungal growth rate and help faster identification in patients with positive blood cultures with Candida species. In this study, our aim is to determine the distribution of yeasts isolated from blood cultures and to determine their growth times, to compare the identification times by MALDI-TOF MS method and by the carbohydrate assimilation method, and to develop new approaches to accelerate their growing time in blood culture bottles.
Materials and Methods: In our study, the special fungal medium, which was thought to accelerate the growth of yeasts, was prepared. Serial dilutions of C. albicans ATCC 10231, C. parapsilosis ATCC 22019, C. glabrata ATCC 90030 strains were prepared and inoculated in special fungal medium and standard blood culture bottles.
Results: The mean time to positivity in blood culture bottles supplemented with special fungal medium was compared to blood culture bottles without supplementation. It was observed that the addition of a special fungal medium to the aerobic bottles shortened about two hours the growing time of C. albicans. However, no significant difference was found when the mean growth times of the bottles with special fungal medium added and the bottles without added were biostatistically compared, regardless of strain. (p> 0.05). The identification of the colonies by MALDI-TOF MS shortened the identification time about 21.5 hours when compared to the classical methods.
Conclusion: Blood culture is the gold standard method in the diagnosis of candidemia. If the identification and the susceptibility testing periods are added into the growth time, the implementation of targeted therapy exceeds 36-48 hours. Any approaches for shortening this period would be life-saving procedures. |
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| ISSN: | 1300-932X 1308-5115 |