Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts
<i>Botrytis cinerea</i> is a necrotrophic fungal phytopathogen with devastating effects on many <i>Vitis</i> genotypes. Here, a screening of 81 <i>Vitis</i> genotypes for leaf resistance to <i>B</i>. <i>cinerea</i> revealed two highly resis...
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/1/5 |
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| author | Mati Ur Rahman Muhammad Hanif Ran Wan Xiaoqing Hou Bilal Ahmad Xiping Wang |
| author_facet | Mati Ur Rahman Muhammad Hanif Ran Wan Xiaoqing Hou Bilal Ahmad Xiping Wang |
| author_sort | Mati Ur Rahman |
| collection | DOAJ |
| description | <i>Botrytis cinerea</i> is a necrotrophic fungal phytopathogen with devastating effects on many <i>Vitis</i> genotypes. Here, a screening of 81 <i>Vitis</i> genotypes for leaf resistance to <i>B</i>. <i>cinerea</i> revealed two highly resistant (HR), twelve resistant (R), twenty-five susceptible (S) and forty-two highly susceptible (HS) genotypes. We focused on the HR genotype, ‘Zi Qiu’ (<i>Vitis davidii</i>), and the HS genotype ‘Riesling’ (<i>V</i>. <i>vinifera</i>), to elucidate mechanisms of host resistance and susceptibility against <i>B</i>. <i>cinerea</i>, using detached leaf assays. These involved a comparison of fungal growth, reactive oxygen species (ROS) responses, jasmonic acid (JA) levels, and changes in the anti-oxidative system between the two genotypes after inoculation with <i>B</i>. <i>cinerea</i>. Our results indicated that the high-level resistance of ‘Zi Qiu’ can be attributed to insignificant fungal development, low ROS production, timely elevation of anti-oxidative functions, and high JA levels. Moreover, severe fungal infection of ‘Riesling’ and sustained ROS production coincided with relatively unchanged anti-oxidative activity, as well as low JA levels. This study provides insights into <i>B</i>. <i>cinerea</i> infection in grape, which can be valuable for breeders by providing information for selecting suitable germplasm with enhanced disease resistance. |
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| spelling | doaj.art-ba9c26cbe4a44ff3920c6a669125483f2022-12-21T18:37:26ZengMDPI AGMolecules1420-30492018-12-01241510.3390/molecules24010005molecules24010005Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible HostsMati Ur Rahman0Muhammad Hanif1Ran Wan2Xiaoqing Hou3Bilal Ahmad4Xiping Wang5State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China<i>Botrytis cinerea</i> is a necrotrophic fungal phytopathogen with devastating effects on many <i>Vitis</i> genotypes. Here, a screening of 81 <i>Vitis</i> genotypes for leaf resistance to <i>B</i>. <i>cinerea</i> revealed two highly resistant (HR), twelve resistant (R), twenty-five susceptible (S) and forty-two highly susceptible (HS) genotypes. We focused on the HR genotype, ‘Zi Qiu’ (<i>Vitis davidii</i>), and the HS genotype ‘Riesling’ (<i>V</i>. <i>vinifera</i>), to elucidate mechanisms of host resistance and susceptibility against <i>B</i>. <i>cinerea</i>, using detached leaf assays. These involved a comparison of fungal growth, reactive oxygen species (ROS) responses, jasmonic acid (JA) levels, and changes in the anti-oxidative system between the two genotypes after inoculation with <i>B</i>. <i>cinerea</i>. Our results indicated that the high-level resistance of ‘Zi Qiu’ can be attributed to insignificant fungal development, low ROS production, timely elevation of anti-oxidative functions, and high JA levels. Moreover, severe fungal infection of ‘Riesling’ and sustained ROS production coincided with relatively unchanged anti-oxidative activity, as well as low JA levels. This study provides insights into <i>B</i>. <i>cinerea</i> infection in grape, which can be valuable for breeders by providing information for selecting suitable germplasm with enhanced disease resistance.https://www.mdpi.com/1420-3049/24/1/5biotic stress<i>Botrytis cinerea</i>graperesistant genotypesreactive oxygen speciesjasmonic acidantioxidant enzymes |
| spellingShingle | Mati Ur Rahman Muhammad Hanif Ran Wan Xiaoqing Hou Bilal Ahmad Xiping Wang Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts Molecules biotic stress <i>Botrytis cinerea</i> grape resistant genotypes reactive oxygen species jasmonic acid antioxidant enzymes |
| title | Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts |
| title_full | Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts |
| title_fullStr | Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts |
| title_full_unstemmed | Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts |
| title_short | Screening <i>Vitis</i> Genotypes for Responses to <i>Botrytis cinerea</i> and Evaluation of Antioxidant Enzymes, Reactive Oxygen Species and Jasmonic Acid in Resistant and Susceptible Hosts |
| title_sort | screening i vitis i genotypes for responses to i botrytis cinerea i and evaluation of antioxidant enzymes reactive oxygen species and jasmonic acid in resistant and susceptible hosts |
| topic | biotic stress <i>Botrytis cinerea</i> grape resistant genotypes reactive oxygen species jasmonic acid antioxidant enzymes |
| url | https://www.mdpi.com/1420-3049/24/1/5 |
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