Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i>
In this study, we explored the mechanism underlying <i>Aspergillus flavus</i> conidia germination inhibited by decreased water activity. The impact of low water activity was analyzed at 4 h, 8 h and 12 h. Additionally, we demonstrated that low water activity affected cell shape and decre...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1744 |
| _version_ | 1797484564967325696 |
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| author | Sifan Jia Chong Li Kuntan Wu Desheng Qi Shuai Wang |
| author_facet | Sifan Jia Chong Li Kuntan Wu Desheng Qi Shuai Wang |
| author_sort | Sifan Jia |
| collection | DOAJ |
| description | In this study, we explored the mechanism underlying <i>Aspergillus flavus</i> conidia germination inhibited by decreased water activity. The impact of low water activity was analyzed at 4 h, 8 h and 12 h. Additionally, we demonstrated that low water activity affected cell shape and decreased cell sizes. Transcriptomics found numerous differentially expressed genes (DEGs) during the first 12 h of germination, with 654 DEGs observed among 4 h, 8 h and 12 h. In particular, more DEGs were detected at 8 h of germinating. Therefore, proteomics was performed at 8 h, and 209 differentially expressed proteins (DEPs) were speculated, with 94 up-regulated and 115 down-regulated. Combined analysis of KEGG of transcriptomics and proteomics demonstrated that the dominant pathways were nutrient metabolism and translation. We also found several DEGs and DEPs in the Mitogen Activated Protein Kinase (MAPK) pathway. Therefore, we concluded that low water activity inhibited conidia germination, causing unregular morphology. In addition, low water activity influenced expression of <i>creA</i>, <i>TreB</i> in carbohydrate metabolism, <i>Clr4</i>, <i>RmtA</i> in amino acid metabolism and <i>RPL37, RPL3</i> in translation in <i>Aspergillus flavus</i>. |
| first_indexed | 2024-03-09T23:07:11Z |
| format | Article |
| id | doaj.art-baa216dcf08646bea0f3d8c9ac7f8212 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:07:11Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-baa216dcf08646bea0f3d8c9ac7f82122023-11-23T17:52:31ZengMDPI AGMicroorganisms2076-26072022-08-01109174410.3390/microorganisms10091744Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i>Sifan Jia0Chong Li1Kuntan Wu2Desheng Qi3Shuai Wang4Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaIn this study, we explored the mechanism underlying <i>Aspergillus flavus</i> conidia germination inhibited by decreased water activity. The impact of low water activity was analyzed at 4 h, 8 h and 12 h. Additionally, we demonstrated that low water activity affected cell shape and decreased cell sizes. Transcriptomics found numerous differentially expressed genes (DEGs) during the first 12 h of germination, with 654 DEGs observed among 4 h, 8 h and 12 h. In particular, more DEGs were detected at 8 h of germinating. Therefore, proteomics was performed at 8 h, and 209 differentially expressed proteins (DEPs) were speculated, with 94 up-regulated and 115 down-regulated. Combined analysis of KEGG of transcriptomics and proteomics demonstrated that the dominant pathways were nutrient metabolism and translation. We also found several DEGs and DEPs in the Mitogen Activated Protein Kinase (MAPK) pathway. Therefore, we concluded that low water activity inhibited conidia germination, causing unregular morphology. In addition, low water activity influenced expression of <i>creA</i>, <i>TreB</i> in carbohydrate metabolism, <i>Clr4</i>, <i>RmtA</i> in amino acid metabolism and <i>RPL37, RPL3</i> in translation in <i>Aspergillus flavus</i>.https://www.mdpi.com/2076-2607/10/9/1744water activity<i>Aspergillus flavus</i>conidia germinationtranscriptomicsproteomics |
| spellingShingle | Sifan Jia Chong Li Kuntan Wu Desheng Qi Shuai Wang Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i> Microorganisms water activity <i>Aspergillus flavus</i> conidia germination transcriptomics proteomics |
| title | Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i> |
| title_full | Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i> |
| title_fullStr | Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i> |
| title_full_unstemmed | Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i> |
| title_short | Effect of Water Activity on Conidia Germination in <i>Aspergillus flavus</i> |
| title_sort | effect of water activity on conidia germination in i aspergillus flavus i |
| topic | water activity <i>Aspergillus flavus</i> conidia germination transcriptomics proteomics |
| url | https://www.mdpi.com/2076-2607/10/9/1744 |
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