Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression
The corm rot of saffron caused by <i>Fusarium oxysporum</i> (Fox) has been reported to be the most destructive fungal disease of the herb globally. The pathogen, <i>Fusarium oxysporum</i> R1 (Fox R1) isolated by our group from Kashmir, India, was found to be different from &l...
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MDPI AG
2022-11-01
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| Series: | Journal of Fungi |
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| author | Nancy Bhagat Shanu Magotra Rikita Gupta Shikha Sharma Sandhya Verma Praveen Kumar Verma Tahir Ali Ankita Shree Jyoti Vakhlu |
| author_facet | Nancy Bhagat Shanu Magotra Rikita Gupta Shikha Sharma Sandhya Verma Praveen Kumar Verma Tahir Ali Ankita Shree Jyoti Vakhlu |
| author_sort | Nancy Bhagat |
| collection | DOAJ |
| description | The corm rot of saffron caused by <i>Fusarium oxysporum</i> (Fox) has been reported to be the most destructive fungal disease of the herb globally. The pathogen, <i>Fusarium oxysporum</i> R1 (Fox R1) isolated by our group from Kashmir, India, was found to be different from <i>Fusarium oxysporum</i> f.sp. <i>gladioli</i> commonly reported corm rot agent of saffron. In the present study, Fox R1 was further characterized using housekeeping genes and pathogenicity tests, as <i>Fusarium oxysporum</i> R1 f.sp. <i>iridacearum</i> race 4. Though Fox R1 invaded the saffron plant through both corm and roots, the corm was found to be the preferred site of infection. In addition, the route of pathogen movement wastracked by monitoring visual symptoms, semi-quantitative PCR, quantitative-PCR (q-PCR), real-time imaging of <i>egfp</i>-tagged <i>Fusarium oxysporum</i> R1, and Fox R1 load quantification. This study is the first study of its kind on the bidirectional pathogenesis from corm to roots and vice-versa, as the literature only reports unidirectional upward movement from roots to other parts of the plant. In addition, the colonization pattern of Fox R1 in saffron corms and roots was studied. The present study involved a systematic elucidation of the mode and mechanism of pathogenesis in the saffron <i>Fusarium oxysporum</i> strain R1 pathosystem. |
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| publishDate | 2022-11-01 |
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| spelling | doaj.art-baa48a0850e1464bab5821ccd0677ec32023-11-24T15:59:12ZengMDPI AGJournal of Fungi2309-608X2022-11-01812124610.3390/jof8121246Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease ProgressionNancy Bhagat0Shanu Magotra1Rikita Gupta2Shikha Sharma3Sandhya Verma4Praveen Kumar Verma5Tahir Ali6Ankita Shree7Jyoti Vakhlu8Metagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, IndiaMetagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, IndiaMetagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, IndiaMetagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, IndiaPlant Immunity Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi 110067, IndiaPlant Immunity Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi 110067, IndiaMetagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, IndiaPlant Immunity Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi 110067, IndiaMetagenomic Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, IndiaThe corm rot of saffron caused by <i>Fusarium oxysporum</i> (Fox) has been reported to be the most destructive fungal disease of the herb globally. The pathogen, <i>Fusarium oxysporum</i> R1 (Fox R1) isolated by our group from Kashmir, India, was found to be different from <i>Fusarium oxysporum</i> f.sp. <i>gladioli</i> commonly reported corm rot agent of saffron. In the present study, Fox R1 was further characterized using housekeeping genes and pathogenicity tests, as <i>Fusarium oxysporum</i> R1 f.sp. <i>iridacearum</i> race 4. Though Fox R1 invaded the saffron plant through both corm and roots, the corm was found to be the preferred site of infection. In addition, the route of pathogen movement wastracked by monitoring visual symptoms, semi-quantitative PCR, quantitative-PCR (q-PCR), real-time imaging of <i>egfp</i>-tagged <i>Fusarium oxysporum</i> R1, and Fox R1 load quantification. This study is the first study of its kind on the bidirectional pathogenesis from corm to roots and vice-versa, as the literature only reports unidirectional upward movement from roots to other parts of the plant. In addition, the colonization pattern of Fox R1 in saffron corms and roots was studied. The present study involved a systematic elucidation of the mode and mechanism of pathogenesis in the saffron <i>Fusarium oxysporum</i> strain R1 pathosystem.https://www.mdpi.com/2309-608X/8/12/1246<i>Crocus sativus</i><i>Fusarium oxysporum</i>corm-rot of saffronmolecular identificationhousekeeping genes |
| spellingShingle | Nancy Bhagat Shanu Magotra Rikita Gupta Shikha Sharma Sandhya Verma Praveen Kumar Verma Tahir Ali Ankita Shree Jyoti Vakhlu Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression Journal of Fungi <i>Crocus sativus</i> <i>Fusarium oxysporum</i> corm-rot of saffron molecular identification housekeeping genes |
| title | Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression |
| title_full | Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression |
| title_fullStr | Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression |
| title_full_unstemmed | Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression |
| title_short | Invasion and Colonization of Pathogenic <i>Fusarium oxysporum</i> R1 in <i>Crocus sativus</i> L. during Corm Rot Disease Progression |
| title_sort | invasion and colonization of pathogenic i fusarium oxysporum i r1 in i crocus sativus i l during corm rot disease progression |
| topic | <i>Crocus sativus</i> <i>Fusarium oxysporum</i> corm-rot of saffron molecular identification housekeeping genes |
| url | https://www.mdpi.com/2309-608X/8/12/1246 |
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