Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23
Bioremediation is promising technology for dealing with oil hydrocarbons contamination. In this research growth kinetics and oil biodegradation efficiency of Pseudomonas luteola PRO23, isolated from crude oil-contaminated soil samples, were investigated under different concentrations (5, 10...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Association of Chemical Engineers of Serbia
2016-01-01
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| Series: | Hemijska Industrija |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2016/0367-598X1500020A.pdf |
| Summary: | Bioremediation is promising technology for dealing with oil hydrocarbons
contamination. In this research growth kinetics and oil biodegradation
efficiency of Pseudomonas luteola PRO23, isolated from crude oil-contaminated
soil samples, were investigated under different concentrations (5, 10 and 20
g/L) of light and heavy crude oil. More efficient biodegradation and more
rapid adaptation and cell growth were obtained in conditions with light oil.
The 5 to 10 g/L upgrade of light oil concentration stimulated the microbial
growth and the biodegradation efficiency. Further upgrade of light oil
concentration and the upgrade of heavy oil concentration both inhibited the
microbial growth, as well as biodegradation process. Aminoglycosides
stimulated biosurfactant production in P. luteola in the range of
sub-inhibitory concentrations (0.3125, 0.625 μg/mL). Aminoglycosides also
induced biofilm formation. The production of biosurfactants was the most
intense during lag phase and continues until stationary phase.
Aminoglycosides also induced changes in P. luteola growth kinetics. In the
presence of aminoglycosides this strain degraded 82% of diesel for 96 h.
These results indicated that Pseudomonas luteola PRO23 potentially can be
used in bioremediation of crude oil-contaminated environments and that
aminoglycosides could stimulate this process. [Projekat Ministarstva nauke
Republike Srbije, br. TR31080] |
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| ISSN: | 0367-598X 2217-7426 |