Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms
Selective labeling of distinct bacteria and biofilm is poised for the fundamental understanding of bacterial activities, interactions, and coupled phenomena occurring at the microscale. However, a simple and effective way to achieve selective bacterial labeling is still lacking. Herein, we report a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.971682/full |
| _version_ | 1798043017388490752 |
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| author | Jiayi Lin Linlin Xu Yuling Zheng Dalin Wu Jun Yue |
| author_facet | Jiayi Lin Linlin Xu Yuling Zheng Dalin Wu Jun Yue |
| author_sort | Jiayi Lin |
| collection | DOAJ |
| description | Selective labeling of distinct bacteria and biofilm is poised for the fundamental understanding of bacterial activities, interactions, and coupled phenomena occurring at the microscale. However, a simple and effective way to achieve selective bacterial labeling is still lacking. Herein, we report a fluorescence probe with core-shell nanostructure that has polydopamine (PDA) coating on the surface of fluorescent silicon quantum dots (SiQDs@PDA). The surface of the SiQDs@PDA can be functionalized by various molecules (2-mercaptoethylamine hydrochloride, PEG, d-alanine, glucose amide) through different strategies (Michael addition, π-π interaction, and ion–ion interaction). Importantly, the d-alanine (D-Ala)- and gluconamide (Glc)-functionalized SiQDs@PDA fluorescence probes are capable of selectively labeling gram-positive and gram-negative bacteria, as well as their biofilms. The excellent performance in universal functionalization and selective labeling and imaging of bacteria and their biofilms demonstrate that SiQDs@PDA are a promising fluorescence tool in microbe research. |
| first_indexed | 2024-04-11T22:43:47Z |
| format | Article |
| id | doaj.art-baa73aec4a94489486985cc29a9ab492 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T22:43:47Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-baa73aec4a94489486985cc29a9ab4922022-12-22T03:58:53ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-08-011010.3389/fbioe.2022.971682971682Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilmsJiayi LinLinlin XuYuling ZhengDalin WuJun YueSelective labeling of distinct bacteria and biofilm is poised for the fundamental understanding of bacterial activities, interactions, and coupled phenomena occurring at the microscale. However, a simple and effective way to achieve selective bacterial labeling is still lacking. Herein, we report a fluorescence probe with core-shell nanostructure that has polydopamine (PDA) coating on the surface of fluorescent silicon quantum dots (SiQDs@PDA). The surface of the SiQDs@PDA can be functionalized by various molecules (2-mercaptoethylamine hydrochloride, PEG, d-alanine, glucose amide) through different strategies (Michael addition, π-π interaction, and ion–ion interaction). Importantly, the d-alanine (D-Ala)- and gluconamide (Glc)-functionalized SiQDs@PDA fluorescence probes are capable of selectively labeling gram-positive and gram-negative bacteria, as well as their biofilms. The excellent performance in universal functionalization and selective labeling and imaging of bacteria and their biofilms demonstrate that SiQDs@PDA are a promising fluorescence tool in microbe research.https://www.frontiersin.org/articles/10.3389/fbioe.2022.971682/fullselective labelingbacteriabacterial biofilmfluorescencesilicon quantum dots |
| spellingShingle | Jiayi Lin Linlin Xu Yuling Zheng Dalin Wu Jun Yue Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms Frontiers in Bioengineering and Biotechnology selective labeling bacteria bacterial biofilm fluorescence silicon quantum dots |
| title | Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms |
| title_full | Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms |
| title_fullStr | Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms |
| title_full_unstemmed | Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms |
| title_short | Imitation-mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms |
| title_sort | imitation mussel fluorescent silicon quantum dots for selective labeling and imaging of bacteria and biofilms |
| topic | selective labeling bacteria bacterial biofilm fluorescence silicon quantum dots |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.971682/full |
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