An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-12-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-020-01478-z |
| _version_ | 1818839674834124800 |
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| author | Hongmin Cai Hebang Yao Tingting Li Cedric A. J. Hutter Yanfang Li Yannan Tang Markus A. Seeger Dianfan Li |
| author_facet | Hongmin Cai Hebang Yao Tingting Li Cedric A. J. Hutter Yanfang Li Yannan Tang Markus A. Seeger Dianfan Li |
| author_sort | Hongmin Cai |
| collection | DOAJ |
| description | In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification. |
| first_indexed | 2024-12-19T03:58:03Z |
| format | Article |
| id | doaj.art-baab4a34e2bb410bbf23d2caba607e37 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-19T03:58:03Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-baab4a34e2bb410bbf23d2caba607e372022-12-21T20:36:46ZengNature PortfolioCommunications Biology2399-36422020-12-013111610.1038/s42003-020-01478-zAn improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purificationHongmin Cai0Hebang Yao1Tingting Li2Cedric A. J. Hutter3Yanfang Li4Yannan Tang5Markus A. Seeger6Dianfan Li7University of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesUniversity of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesUniversity of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesInstitute of Medical Microbiology, University of ZurichUniversity of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesUniversity of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesInstitute of Medical Microbiology, University of ZurichUniversity of Chinese Academy of Sciences, National Center for Protein Science Shanghai, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesIn this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification.https://doi.org/10.1038/s42003-020-01478-z |
| spellingShingle | Hongmin Cai Hebang Yao Tingting Li Cedric A. J. Hutter Yanfang Li Yannan Tang Markus A. Seeger Dianfan Li An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification Communications Biology |
| title | An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification |
| title_full | An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification |
| title_fullStr | An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification |
| title_full_unstemmed | An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification |
| title_short | An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification |
| title_sort | improved fluorescent tag and its nanobodies for membrane protein expression stability assay and purification |
| url | https://doi.org/10.1038/s42003-020-01478-z |
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