A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix
Currently, the detection of pathogens such as <i>Escherichia coli</i> through instrumental alternatives with fast response and excellent sensitivity and selectivity are being studied. Biosensors are systems consisting of nanomaterials and biomolecules that exhibit remarkable properties s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/9/2559 |
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| author | Jose Luis Ropero-Vega Joshua Felipe Redondo-Ortega Yuli Juliana Galvis-Curubo Paola Rondón-Villarreal Johanna Marcela Flórez-Castillo |
| author_facet | Jose Luis Ropero-Vega Joshua Felipe Redondo-Ortega Yuli Juliana Galvis-Curubo Paola Rondón-Villarreal Johanna Marcela Flórez-Castillo |
| author_sort | Jose Luis Ropero-Vega |
| collection | DOAJ |
| description | Currently, the detection of pathogens such as <i>Escherichia coli</i> through instrumental alternatives with fast response and excellent sensitivity and selectivity are being studied. Biosensors are systems consisting of nanomaterials and biomolecules that exhibit remarkable properties such as simplicity, portable, affordable, user‑friendly, and deliverable to end‑users. For this, in this work we report for the first time, to our knowledge, the bioinformatic design of a new peptide based on TIR protein, a receptor of Intimin membrane protein which is characteristic of <i>E. coli</i>. This peptide (named PEPTIR‑1.0) was used as recognition element in a biosensor based on AuNPs‑modified screen‑printed electrodes for the detection of <i>E. coli</i>. The morphological and electrochemical characteristics of the biosensor obtained were studied. Results show that the biosensor can detect the bacteria with limits of detection and quantification of 2 and 6 CFU/mL, respectively. Moreover, the selectivity of the system is statistically significant towards the detection of the pathogen in the presence of other microorganisms such as <i>P. aeruginosa</i> and <i>S. aureus</i>. This makes this new PEPTIR‑1.0 based biosensor can be used in the rapid, sensitive, and selective detection of <i>E. coli</i> in aqueous matrices. |
| first_indexed | 2024-03-10T11:54:25Z |
| format | Article |
| id | doaj.art-baac7ef00d4943c6892d12b7d3c13b33 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:54:25Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-baac7ef00d4943c6892d12b7d3c13b332023-11-21T17:28:45ZengMDPI AGMolecules1420-30492021-04-01269255910.3390/molecules26092559A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous MatrixJose Luis Ropero-Vega0Joshua Felipe Redondo-Ortega1Yuli Juliana Galvis-Curubo2Paola Rondón-Villarreal3Johanna Marcela Flórez-Castillo4Facultad de Ciencias Exactas, Naturales y Agropecuarias, Ciencias Básicas y Aplicadas Para la Sostenibilidad—CIBAS, Universidad de Santander, Calle 70 No. 55-210, Bucaramanga C.P. 680003, Santander, ColombiaFacultad de Ciencias Exactas, Naturales y Agropecuarias, Ciencias Básicas y Aplicadas Para la Sostenibilidad—CIBAS, Universidad de Santander, Calle 70 No. 55-210, Bucaramanga C.P. 680003, Santander, ColombiaFacultad de Ciencias Exactas, Naturales y Agropecuarias, Ciencias Básicas y Aplicadas Para la Sostenibilidad—CIBAS, Universidad de Santander, Calle 70 No. 55-210, Bucaramanga C.P. 680003, Santander, ColombiaFacultad de Ciencias de la Salud, Grupo de Investigación en Biología Molecular y Biotecnología, Universidad de Santander, Calle 70 No. 55-210, Bucaramanga C.P. 680003, Santander, ColombiaFacultad de Ciencias Exactas, Naturales y Agropecuarias, Ciencias Básicas y Aplicadas Para la Sostenibilidad—CIBAS, Universidad de Santander, Calle 70 No. 55-210, Bucaramanga C.P. 680003, Santander, ColombiaCurrently, the detection of pathogens such as <i>Escherichia coli</i> through instrumental alternatives with fast response and excellent sensitivity and selectivity are being studied. Biosensors are systems consisting of nanomaterials and biomolecules that exhibit remarkable properties such as simplicity, portable, affordable, user‑friendly, and deliverable to end‑users. For this, in this work we report for the first time, to our knowledge, the bioinformatic design of a new peptide based on TIR protein, a receptor of Intimin membrane protein which is characteristic of <i>E. coli</i>. This peptide (named PEPTIR‑1.0) was used as recognition element in a biosensor based on AuNPs‑modified screen‑printed electrodes for the detection of <i>E. coli</i>. The morphological and electrochemical characteristics of the biosensor obtained were studied. Results show that the biosensor can detect the bacteria with limits of detection and quantification of 2 and 6 CFU/mL, respectively. Moreover, the selectivity of the system is statistically significant towards the detection of the pathogen in the presence of other microorganisms such as <i>P. aeruginosa</i> and <i>S. aureus</i>. This makes this new PEPTIR‑1.0 based biosensor can be used in the rapid, sensitive, and selective detection of <i>E. coli</i> in aqueous matrices.https://www.mdpi.com/1420-3049/26/9/2559pathogenPEPTIR-1.0screen-printed electrodesgold nanoparticleselectrodepositionlimit of detection |
| spellingShingle | Jose Luis Ropero-Vega Joshua Felipe Redondo-Ortega Yuli Juliana Galvis-Curubo Paola Rondón-Villarreal Johanna Marcela Flórez-Castillo A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix Molecules pathogen PEPTIR-1.0 screen-printed electrodes gold nanoparticles electrodeposition limit of detection |
| title | A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix |
| title_full | A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix |
| title_fullStr | A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix |
| title_full_unstemmed | A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix |
| title_short | A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of <i>E. coli</i> O157:H7 in an Aqueous Matrix |
| title_sort | bioinspired peptide in tir protein as recognition molecule on electrochemical biosensors for the detection of i e coli i o157 h7 in an aqueous matrix |
| topic | pathogen PEPTIR-1.0 screen-printed electrodes gold nanoparticles electrodeposition limit of detection |
| url | https://www.mdpi.com/1420-3049/26/9/2559 |
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