Continuous fluorescence assay for lecithin:cholesterol acyltransferase using a water-soluble phosphatidylcholine.

A water-soluble fluorescent phosphatidylcholine, 1,2-bis[4-(1-pyreno-butanoyl]-sn-glycero-3-phosphocholine (DPybPC) has been used to develop a sensitive, continuous assay for pure lecithin:cholesterol acyltransferase (LCAT) in solution. The monomeric substrate allowed us to examine the reaction of L...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: FS Bonelli, A Jonas
Format: Artikel
Sprache:English
Veröffentlicht: Elsevier 1992-12-01
Schriftenreihe:Journal of Lipid Research
Online Zugang:http://www.sciencedirect.com/science/article/pii/S0022227520413446