Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites
Objective The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2021-11-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-20-0637.pdf |
| _version_ | 1818404774539689984 |
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| author | Jinhua Wu Ronghui Liu Hua Li Hui Yu Yalan Yang |
| author_facet | Jinhua Wu Ronghui Liu Hua Li Hui Yu Yalan Yang |
| author_sort | Jinhua Wu |
| collection | DOAJ |
| description | Objective The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are limited. Methods Eighteen pairs of microsatellite primers were used to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The genetic differentiation coefficient (Fst) and neighbor-joining methods were used for cluster analysis of the breeds. Results In miniature pigs, the SLA I region had the highest numbers of polymorphisms, followed by the SLA II and SLA III regions; the region near the centromere had the lowest number of polymorphisms. Among the seven miniature pig breeds, Diannan small-ear pigs had the highest genetic diversity (PIC value = 0.6396), whereas the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst values in the Mingguang small-ear, Diannan small-ear, and Yunnan wild boars were less than 0.05. According to phylogenetic cluster analysis, the South-China-type miniature pigs clustered into one group, among which Mingguang small-ear pigs clustered with Diannan small-ear pigs. Haplotype analysis revealed that the SLA I, II, and III regions could be constructed into 13, 7, and 11 common haplotypes, respectively. Conclusion This study validates the high genetic diversity of the Chinese miniature pig. Mingguang small-ear pigs have close kinship with Diannan small-ear pigs, implying that they may have similar genetic backgrounds and originate from the same population. This study also provides a foundation for genetic breeding, genetic resource protection, and classification of Chinese miniature pigs. |
| first_indexed | 2024-12-14T08:45:30Z |
| format | Article |
| id | doaj.art-bab1aa55b67e43c688452936393339ed |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-12-14T08:45:30Z |
| publishDate | 2021-11-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-bab1aa55b67e43c688452936393339ed2022-12-21T23:09:12ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352021-11-0134111757176510.5713/ab.20.063724678Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellitesJinhua Wu0Ronghui Liu1Hua Li2Hui Yu3Yalan Yang4 Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan 528225, China Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan 528225, China Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan 528225, China Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan 528225, China Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan 528225, ChinaObjective The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are limited. Methods Eighteen pairs of microsatellite primers were used to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The genetic differentiation coefficient (Fst) and neighbor-joining methods were used for cluster analysis of the breeds. Results In miniature pigs, the SLA I region had the highest numbers of polymorphisms, followed by the SLA II and SLA III regions; the region near the centromere had the lowest number of polymorphisms. Among the seven miniature pig breeds, Diannan small-ear pigs had the highest genetic diversity (PIC value = 0.6396), whereas the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst values in the Mingguang small-ear, Diannan small-ear, and Yunnan wild boars were less than 0.05. According to phylogenetic cluster analysis, the South-China-type miniature pigs clustered into one group, among which Mingguang small-ear pigs clustered with Diannan small-ear pigs. Haplotype analysis revealed that the SLA I, II, and III regions could be constructed into 13, 7, and 11 common haplotypes, respectively. Conclusion This study validates the high genetic diversity of the Chinese miniature pig. Mingguang small-ear pigs have close kinship with Diannan small-ear pigs, implying that they may have similar genetic backgrounds and originate from the same population. This study also provides a foundation for genetic breeding, genetic resource protection, and classification of Chinese miniature pigs.http://www.animbiosci.org/upload/pdf/ab-20-0637.pdfdiversityhaplotypesmicrosatelliteminiature pigsswine leukocyte antigen (sla) |
| spellingShingle | Jinhua Wu Ronghui Liu Hua Li Hui Yu Yalan Yang Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites Animal Bioscience diversity haplotypes microsatellite miniature pigs swine leukocyte antigen (sla) |
| title | Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites |
| title_full | Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites |
| title_fullStr | Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites |
| title_full_unstemmed | Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites |
| title_short | Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites |
| title_sort | genetic diversity analysis in chinese miniature pigs using swine leukocyte antigen complex microsatellites |
| topic | diversity haplotypes microsatellite miniature pigs swine leukocyte antigen (sla) |
| url | http://www.animbiosci.org/upload/pdf/ab-20-0637.pdf |
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