The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses.
The limits of life of aerobic microorganisms are well understood, but the responses of anaerobic microorganisms to individual and combined extreme stressors are less well known. Motivated by an interest in understanding the survivability of anaerobic microorganisms under Martian conditions, we inves...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5656303?pdf=render |
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| author | Kristina Beblo-Vranesevic Maria Bohmeier Alexandra K Perras Petra Schwendner Elke Rabbow Christine Moissl-Eichinger Charles S Cockell Rüdiger Pukall Pauline Vannier Viggo T Marteinsson Euan P Monaghan Pascale Ehrenfreund Laura Garcia-Descalzo Felipe Gómez Moustafa Malki Ricardo Amils Frédéric Gaboyer Frances Westall Patricia Cabezas Nicolas Walter Petra Rettberg |
| author_facet | Kristina Beblo-Vranesevic Maria Bohmeier Alexandra K Perras Petra Schwendner Elke Rabbow Christine Moissl-Eichinger Charles S Cockell Rüdiger Pukall Pauline Vannier Viggo T Marteinsson Euan P Monaghan Pascale Ehrenfreund Laura Garcia-Descalzo Felipe Gómez Moustafa Malki Ricardo Amils Frédéric Gaboyer Frances Westall Patricia Cabezas Nicolas Walter Petra Rettberg |
| author_sort | Kristina Beblo-Vranesevic |
| collection | DOAJ |
| description | The limits of life of aerobic microorganisms are well understood, but the responses of anaerobic microorganisms to individual and combined extreme stressors are less well known. Motivated by an interest in understanding the survivability of anaerobic microorganisms under Martian conditions, we investigated the responses of a new isolate, Yersinia intermedia MASE-LG-1 to individual and combined stresses associated with the Martian surface. This organism belongs to an adaptable and persistent genus of anaerobic microorganisms found in many environments worldwide. The effects of desiccation, low pressure, ionizing radiation, varying temperature, osmotic pressure, and oxidizing chemical compounds were investigated. The strain showed a high tolerance to desiccation, with a decline of survivability by four orders of magnitude during a storage time of 85 days. Exposure to X-rays resulted in dose-dependent inactivation for exposure up to 600 Gy while applied doses above 750 Gy led to complete inactivation. The effects of the combination of desiccation and irradiation were additive and the survivability was influenced by the order in which they were imposed. Ionizing irradiation and subsequent desiccation was more deleterious than vice versa. By contrast, the presence of perchlorates was not found to significantly affect the survival of the Yersinia strain after ionizing radiation. These data show that the organism has the capacity to survive and grow in physical and chemical stresses, imposed individually or in combination that are associated with Martian environment. Eventually it lost its viability showing that many of the most adaptable anaerobic organisms on Earth would be killed on Mars today. |
| first_indexed | 2024-12-23T05:59:50Z |
| format | Article |
| id | doaj.art-bab1bd62dfc64b98a94a3464dbbacafd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T05:59:50Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bab1bd62dfc64b98a94a3464dbbacafd2022-12-21T17:57:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018517810.1371/journal.pone.0185178The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses.Kristina Beblo-VranesevicMaria BohmeierAlexandra K PerrasPetra SchwendnerElke RabbowChristine Moissl-EichingerCharles S CockellRüdiger PukallPauline VannierViggo T MarteinssonEuan P MonaghanPascale EhrenfreundLaura Garcia-DescalzoFelipe GómezMoustafa MalkiRicardo AmilsFrédéric GaboyerFrances WestallPatricia CabezasNicolas WalterPetra RettbergThe limits of life of aerobic microorganisms are well understood, but the responses of anaerobic microorganisms to individual and combined extreme stressors are less well known. Motivated by an interest in understanding the survivability of anaerobic microorganisms under Martian conditions, we investigated the responses of a new isolate, Yersinia intermedia MASE-LG-1 to individual and combined stresses associated with the Martian surface. This organism belongs to an adaptable and persistent genus of anaerobic microorganisms found in many environments worldwide. The effects of desiccation, low pressure, ionizing radiation, varying temperature, osmotic pressure, and oxidizing chemical compounds were investigated. The strain showed a high tolerance to desiccation, with a decline of survivability by four orders of magnitude during a storage time of 85 days. Exposure to X-rays resulted in dose-dependent inactivation for exposure up to 600 Gy while applied doses above 750 Gy led to complete inactivation. The effects of the combination of desiccation and irradiation were additive and the survivability was influenced by the order in which they were imposed. Ionizing irradiation and subsequent desiccation was more deleterious than vice versa. By contrast, the presence of perchlorates was not found to significantly affect the survival of the Yersinia strain after ionizing radiation. These data show that the organism has the capacity to survive and grow in physical and chemical stresses, imposed individually or in combination that are associated with Martian environment. Eventually it lost its viability showing that many of the most adaptable anaerobic organisms on Earth would be killed on Mars today.http://europepmc.org/articles/PMC5656303?pdf=render |
| spellingShingle | Kristina Beblo-Vranesevic Maria Bohmeier Alexandra K Perras Petra Schwendner Elke Rabbow Christine Moissl-Eichinger Charles S Cockell Rüdiger Pukall Pauline Vannier Viggo T Marteinsson Euan P Monaghan Pascale Ehrenfreund Laura Garcia-Descalzo Felipe Gómez Moustafa Malki Ricardo Amils Frédéric Gaboyer Frances Westall Patricia Cabezas Nicolas Walter Petra Rettberg The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. PLoS ONE |
| title | The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. |
| title_full | The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. |
| title_fullStr | The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. |
| title_full_unstemmed | The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. |
| title_short | The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses. |
| title_sort | responses of an anaerobic microorganism yersinia intermedia mase lg 1 to individual and combined simulated martian stresses |
| url | http://europepmc.org/articles/PMC5656303?pdf=render |
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