Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way
Kidney proximal tubules are a key segment in the reabsorption of solutes and water from the glomerular ultrafiltrate, an essential process for maintaining homeostasis in body fluid compartments. The abundant content of Na<sup>+</sup> in the extracellular fluid determines its importance i...
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MDPI AG
2022-02-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/3/66 |
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| author | Lindsey M. P. Cabral Fernando G. Almeida Gloria M. R. S. Grelle Adalberto Vieyra Celso Caruso-Neves Marcelo Einicker-Lamas |
| author_facet | Lindsey M. P. Cabral Fernando G. Almeida Gloria M. R. S. Grelle Adalberto Vieyra Celso Caruso-Neves Marcelo Einicker-Lamas |
| author_sort | Lindsey M. P. Cabral |
| collection | DOAJ |
| description | Kidney proximal tubules are a key segment in the reabsorption of solutes and water from the glomerular ultrafiltrate, an essential process for maintaining homeostasis in body fluid compartments. The abundant content of Na<sup>+</sup> in the extracellular fluid determines its importance in the regulation of extracellular fluid volume, which is particularly important for different physiological processes including blood pressure control. Basolateral membranes of proximal tubule cells have the classic Na<sup>+</sup> + K<sup>+</sup>-ATPase and the ouabain-insensitive, K<sup>+</sup>-insensitive, and furosemide-sensitive Na<sup>+</sup>-ATPase, which participate in the active Na<sup>+</sup> reabsorption. Here, we show that nanomolar concentrations of ceramide-1 phosphate (C1P), a bioactive sphingolipid derived in biological membranes from different metabolic pathways, promotes a strong inhibitory effect on the Na<sup>+</sup>-ATPase activity (C1P<sub>50</sub> ≈ 10 nM), leading to a 72% inhibition of the second sodium pump in the basolateral membranes. Ceramide-1-phosphate directly modulates protein kinase A and protein kinase C, which are known to be involved in the modulation of ion transporters including the renal Na<sup>+</sup>-ATPase. Conversely, we did not observe any effect on the Na<sup>+</sup> + K<sup>+</sup>-ATPase even at a broad C1P concentration range. The significant effect of ceramide-1-phosphate revealed a new potent physiological and pathophysiological modulator for the Na<sup>+</sup>-ATPase, participating in the regulatory network involving glycero- and sphingolipids present in the basolateral membranes of kidney tubule cells. |
| first_indexed | 2024-03-09T19:59:18Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T19:59:18Z |
| publishDate | 2022-02-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-bab25fe31ce04f6da9c28e152b4f4c992023-11-24T00:48:12ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-02-01443998101110.3390/cimb44030066Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic WayLindsey M. P. Cabral0Fernando G. Almeida1Gloria M. R. S. Grelle2Adalberto Vieyra3Celso Caruso-Neves4Marcelo Einicker-Lamas5Laboratório de Biomembranas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, BrazilLaboratório de Biomembranas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, BrazilLaboratório de Biomembranas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, BrazilLaboratório de Físico-Química Biológica Aída Hasson Voloch, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, BrazilLaboratório de Sinalização Celular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, BrazilLaboratório de Biomembranas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-902, BrazilKidney proximal tubules are a key segment in the reabsorption of solutes and water from the glomerular ultrafiltrate, an essential process for maintaining homeostasis in body fluid compartments. The abundant content of Na<sup>+</sup> in the extracellular fluid determines its importance in the regulation of extracellular fluid volume, which is particularly important for different physiological processes including blood pressure control. Basolateral membranes of proximal tubule cells have the classic Na<sup>+</sup> + K<sup>+</sup>-ATPase and the ouabain-insensitive, K<sup>+</sup>-insensitive, and furosemide-sensitive Na<sup>+</sup>-ATPase, which participate in the active Na<sup>+</sup> reabsorption. Here, we show that nanomolar concentrations of ceramide-1 phosphate (C1P), a bioactive sphingolipid derived in biological membranes from different metabolic pathways, promotes a strong inhibitory effect on the Na<sup>+</sup>-ATPase activity (C1P<sub>50</sub> ≈ 10 nM), leading to a 72% inhibition of the second sodium pump in the basolateral membranes. Ceramide-1-phosphate directly modulates protein kinase A and protein kinase C, which are known to be involved in the modulation of ion transporters including the renal Na<sup>+</sup>-ATPase. Conversely, we did not observe any effect on the Na<sup>+</sup> + K<sup>+</sup>-ATPase even at a broad C1P concentration range. The significant effect of ceramide-1-phosphate revealed a new potent physiological and pathophysiological modulator for the Na<sup>+</sup>-ATPase, participating in the regulatory network involving glycero- and sphingolipids present in the basolateral membranes of kidney tubule cells.https://www.mdpi.com/1467-3045/44/3/66Na<sup>+</sup>-ATPaseceramide-1 phosphatekidneyproximal tubulesbasolateral membranessphingolipids |
| spellingShingle | Lindsey M. P. Cabral Fernando G. Almeida Gloria M. R. S. Grelle Adalberto Vieyra Celso Caruso-Neves Marcelo Einicker-Lamas Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way Current Issues in Molecular Biology Na<sup>+</sup>-ATPase ceramide-1 phosphate kidney proximal tubules basolateral membranes sphingolipids |
| title | Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way |
| title_full | Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way |
| title_fullStr | Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way |
| title_full_unstemmed | Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way |
| title_short | Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way |
| title_sort | ceramide 1 phosphate as a potential regulator of the second sodium pump from kidney proximal tubules by triggering distinct protein kinase pathways in a hierarchic way |
| topic | Na<sup>+</sup>-ATPase ceramide-1 phosphate kidney proximal tubules basolateral membranes sphingolipids |
| url | https://www.mdpi.com/1467-3045/44/3/66 |
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