Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis
The causal agent of mal secco disease is the fungus <i>Plenodomus tracheiphilus</i>, mainly affecting lemon tree survival in the Mediterranean area. Using a fully compatible host-pathogen interaction, the aim of our work was to retrieve the fungus transcriptome by an RNA seq approach dur...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/5/761 |
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| author | Angelo Sicilia Riccardo Russo Marco Caruso Carmen Arlotta Silvia Di Silvestro Frederick G. Gmitter Alessandra Gentile Elisabetta Nicolosi Angela Roberta Lo Piero |
| author_facet | Angelo Sicilia Riccardo Russo Marco Caruso Carmen Arlotta Silvia Di Silvestro Frederick G. Gmitter Alessandra Gentile Elisabetta Nicolosi Angela Roberta Lo Piero |
| author_sort | Angelo Sicilia |
| collection | DOAJ |
| description | The causal agent of mal secco disease is the fungus <i>Plenodomus tracheiphilus</i>, mainly affecting lemon tree survival in the Mediterranean area. Using a fully compatible host-pathogen interaction, the aim of our work was to retrieve the fungus transcriptome by an RNA seq approach during infection of rough lemon (<i>Citrus jambhiri</i> Lush.) to identify crucial transcripts for pathogenesis establishment and progression. A total of 2438 clusters belonging to <i>P. tracheiphilus</i> were retrieved and classified into the GO and KEGG categories. Transcripts were categorized mainly within the “membrane”, “catalytic activity”, and “primary metabolic process” GO terms. Moreover, most of the transcripts are included in the “ribosome”, “carbon metabolism”, and “oxidative phosphorylation” KEGG categories. By focusing our attention on transcripts with FPKM values higher than the median, we were able to identify four main transcript groups functioning in (a) fungus cell wall remodeling and protection, (b) destroying plant defensive secondary metabolites, (c) optimizing fungus development and pathogenesis, and (d) toxin biosynthesis, thus indicating that a multifaceted strategy to subdue the host was executed. |
| first_indexed | 2024-03-10T03:18:44Z |
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| id | doaj.art-bab34b1a5205440baba763028ab3b9f2 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T03:18:44Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-bab34b1a5205440baba763028ab3b9f22023-11-23T10:08:05ZengMDPI AGBiology2079-77372022-05-0111576110.3390/biology11050761Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host PathogenesisAngelo Sicilia0Riccardo Russo1Marco Caruso2Carmen Arlotta3Silvia Di Silvestro4Frederick G. Gmitter5Alessandra Gentile6Elisabetta Nicolosi7Angela Roberta Lo Piero8Department of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 98, 95123 Catania, ItalyCitrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, FL 33809, USACouncil for Agricultural Research and Economics, Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia 190, 95024 Acireale, ItalyCouncil for Agricultural Research and Economics, Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia 190, 95024 Acireale, ItalyCouncil for Agricultural Research and Economics, Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia 190, 95024 Acireale, ItalyCitrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, FL 33809, USADepartment of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 98, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 98, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 98, 95123 Catania, ItalyThe causal agent of mal secco disease is the fungus <i>Plenodomus tracheiphilus</i>, mainly affecting lemon tree survival in the Mediterranean area. Using a fully compatible host-pathogen interaction, the aim of our work was to retrieve the fungus transcriptome by an RNA seq approach during infection of rough lemon (<i>Citrus jambhiri</i> Lush.) to identify crucial transcripts for pathogenesis establishment and progression. A total of 2438 clusters belonging to <i>P. tracheiphilus</i> were retrieved and classified into the GO and KEGG categories. Transcripts were categorized mainly within the “membrane”, “catalytic activity”, and “primary metabolic process” GO terms. Moreover, most of the transcripts are included in the “ribosome”, “carbon metabolism”, and “oxidative phosphorylation” KEGG categories. By focusing our attention on transcripts with FPKM values higher than the median, we were able to identify four main transcript groups functioning in (a) fungus cell wall remodeling and protection, (b) destroying plant defensive secondary metabolites, (c) optimizing fungus development and pathogenesis, and (d) toxin biosynthesis, thus indicating that a multifaceted strategy to subdue the host was executed.https://www.mdpi.com/2079-7737/11/5/761<i>Plenodomus tracheiphilus</i>transcriptomemal secco diseasefungus RNAseq<i>Citrus jambhiri</i>rough lemon |
| spellingShingle | Angelo Sicilia Riccardo Russo Marco Caruso Carmen Arlotta Silvia Di Silvestro Frederick G. Gmitter Alessandra Gentile Elisabetta Nicolosi Angela Roberta Lo Piero Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis Biology <i>Plenodomus tracheiphilus</i> transcriptome mal secco disease fungus RNAseq <i>Citrus jambhiri</i> rough lemon |
| title | Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis |
| title_full | Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis |
| title_fullStr | Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis |
| title_full_unstemmed | Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis |
| title_short | Transcriptome Analysis of <i>Plenodomus tracheiphilus</i> Infecting Rough Lemon (<i>Citrus jambhiri</i> Lush.) Indicates a Multifaceted Strategy during Host Pathogenesis |
| title_sort | transcriptome analysis of i plenodomus tracheiphilus i infecting rough lemon i citrus jambhiri i lush indicates a multifaceted strategy during host pathogenesis |
| topic | <i>Plenodomus tracheiphilus</i> transcriptome mal secco disease fungus RNAseq <i>Citrus jambhiri</i> rough lemon |
| url | https://www.mdpi.com/2079-7737/11/5/761 |
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