METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells
<b>Background</b>: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m<sup>6</sup>A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to have regulatory roles in the expression of m&...
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MDPI AG
2022-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/1/113 |
| _version_ | 1797625496014422016 |
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| author | Yeqing Yang Junkai Zeng Chong Jiang Jiawen Chen Ci Song Ming Chen Buling Wu |
| author_facet | Yeqing Yang Junkai Zeng Chong Jiang Jiawen Chen Ci Song Ming Chen Buling Wu |
| author_sort | Yeqing Yang |
| collection | DOAJ |
| description | <b>Background</b>: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m<sup>6</sup>A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to have regulatory roles in the expression of m<sup>6</sup>A regulatory proteins. However, the study on m<sup>6</sup>A modification in hDPSCs has not yet been conducted. <b>Methods</b>: Single base site PCR (MazF) was used to detect the m<sup>6</sup>A modification site of lncSNHG7 before and after mineralization of hDPSCs to screen the target m<sup>6</sup>A modification protein, and bioinformatics analysis was used to analyze the related pathways rich in lncSNHG7. After knockdown and overexpression of lncSNHG7 and METTL3, the osteogenic/odontogenic ability was detected. After METTL3 knockdown, the m<sup>6</sup>A modification level and its expression of lncSNHG7 were detected by MazF, and their binding was confirmed. Finally, the effects of lncSNHG7 and METTL3 on the Wnt/β-catenin pathway were detected. <b>Results</b>: MazF experiments revealed that lncSNHG7 had a m<sup>6</sup>A modification before and after mineralization of hDPSCs, and the occurrence site was 2081. METTL3 was most significantly upregulated after mineralization of hDPSCs. Knockdown/ overexpression of lncSNHG7 and METTL3 inhibited/promoted the osteogenic/odontogenic differentiation of hDPSCs. The m<sup>6</sup>A modification and expression of lncSNHG7 were both regulated by METTL3. Subsequently, lncSNHG7 and METTL3 were found to regulate the Wnt/β-catenin signaling pathway. <b>Conclusion</b>: These results revealed that METTL3 can activate the Wnt/β-catenin signaling pathway by regulating the m<sup>6</sup>A modification and expression of lncSNHG7 in hDPSCs to enhance the osteogenic/odontogenic differentiation of hDPSCs. Our study provides new insight into stem cell-based tissue engineering. |
| first_indexed | 2024-03-11T09:57:15Z |
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| id | doaj.art-bab735e2ea534db58931b8dc17ce3e27 |
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| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-11T09:57:15Z |
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| spelling | doaj.art-bab735e2ea534db58931b8dc17ce3e272023-11-16T15:41:36ZengMDPI AGJournal of Clinical Medicine2077-03832022-12-0112111310.3390/jcm12010113METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem CellsYeqing Yang0Junkai Zeng1Chong Jiang2Jiawen Chen3Ci Song4Ming Chen5Buling Wu6Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, ChinaNanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, ChinaGuangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, ChinaNanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, ChinaNanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, ChinaStomatological Hospital, Southern Medical University, No. 366 Jiangnan Avenue South, Haizhu District, Guangzhou 510280, ChinaNanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, China<b>Background</b>: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m<sup>6</sup>A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to have regulatory roles in the expression of m<sup>6</sup>A regulatory proteins. However, the study on m<sup>6</sup>A modification in hDPSCs has not yet been conducted. <b>Methods</b>: Single base site PCR (MazF) was used to detect the m<sup>6</sup>A modification site of lncSNHG7 before and after mineralization of hDPSCs to screen the target m<sup>6</sup>A modification protein, and bioinformatics analysis was used to analyze the related pathways rich in lncSNHG7. After knockdown and overexpression of lncSNHG7 and METTL3, the osteogenic/odontogenic ability was detected. After METTL3 knockdown, the m<sup>6</sup>A modification level and its expression of lncSNHG7 were detected by MazF, and their binding was confirmed. Finally, the effects of lncSNHG7 and METTL3 on the Wnt/β-catenin pathway were detected. <b>Results</b>: MazF experiments revealed that lncSNHG7 had a m<sup>6</sup>A modification before and after mineralization of hDPSCs, and the occurrence site was 2081. METTL3 was most significantly upregulated after mineralization of hDPSCs. Knockdown/ overexpression of lncSNHG7 and METTL3 inhibited/promoted the osteogenic/odontogenic differentiation of hDPSCs. The m<sup>6</sup>A modification and expression of lncSNHG7 were both regulated by METTL3. Subsequently, lncSNHG7 and METTL3 were found to regulate the Wnt/β-catenin signaling pathway. <b>Conclusion</b>: These results revealed that METTL3 can activate the Wnt/β-catenin signaling pathway by regulating the m<sup>6</sup>A modification and expression of lncSNHG7 in hDPSCs to enhance the osteogenic/odontogenic differentiation of hDPSCs. Our study provides new insight into stem cell-based tissue engineering.https://www.mdpi.com/2077-0383/12/1/113human dental pulp stem cellsN6-methyladenosineosteogenic/odontogenic differentiationRNA epigeneticslong noncoding RNA |
| spellingShingle | Yeqing Yang Junkai Zeng Chong Jiang Jiawen Chen Ci Song Ming Chen Buling Wu METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells Journal of Clinical Medicine human dental pulp stem cells N6-methyladenosine osteogenic/odontogenic differentiation RNA epigenetics long noncoding RNA |
| title | METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells |
| title_full | METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells |
| title_fullStr | METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells |
| title_full_unstemmed | METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells |
| title_short | METTL3-Mediated lncSNHG7 m<sup>6</sup>A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells |
| title_sort | mettl3 mediated lncsnhg7 m sup 6 sup a modification in the osteogenic odontogenic differentiation of human dental stem cells |
| topic | human dental pulp stem cells N6-methyladenosine osteogenic/odontogenic differentiation RNA epigenetics long noncoding RNA |
| url | https://www.mdpi.com/2077-0383/12/1/113 |
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