Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China
Coxsackievirus B5 (CVB5) is an important enterovirus B species (EV-Bs) type. We used the full-length genomic sequences of 53 viral sequences from the national hand, foot, and mouth disease surveillance network in the Chinese mainland (2001–2021). Among them, 69 entire <i>VP1</i> coding r...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/1999-4915/14/12/2693 |
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| author | Yun He Haiyan Wei Leilei Wei Huan Fan Dongmei Yan Hua Zhao Shuangli Zhu Tianjiao Ji Jinbo Xiao Huanhuan Lu Wenhui Wang Qin Guo Qian Yang Weijia Xing Yong Zhang |
| author_facet | Yun He Haiyan Wei Leilei Wei Huan Fan Dongmei Yan Hua Zhao Shuangli Zhu Tianjiao Ji Jinbo Xiao Huanhuan Lu Wenhui Wang Qin Guo Qian Yang Weijia Xing Yong Zhang |
| author_sort | Yun He |
| collection | DOAJ |
| description | Coxsackievirus B5 (CVB5) is an important enterovirus B species (EV-Bs) type. We used the full-length genomic sequences of 53 viral sequences from the national hand, foot, and mouth disease surveillance network in the Chinese mainland (2001–2021). Among them, 69 entire <i>VP1</i> coding region nucleotide sequences were used for CVB5 genotyping and genetic evolution analysis. Phylogenetic analysis based on a data set of 448 complete VP1 sequences showed that CVB5 could be divided into four genotypes (A-D) worldwide. Sequences from this study belonged to genotypes B and D, which dominated transmission in the Chinese mainland. Two transmission lineages of CVB5 have been discovered in the Chinese mainland, lineage 2 was predominant. Markov chain Monte Carlo analysis indicated that the tMRCA of CVB5 in the Chinese mainland could be traced to 1955, while the global trend could be traced to 1862, 93 years earlier than China. The evolution rate of CVB5 was higher in the Chinese mainland than worldwide. The spatiotemporal dynamics analysis of CVB5 assessed that virus transportation events were relatively active in Central, Northeast, North and Northwest China. Recombination analysis revealed frequent intertypic recombination in the non-structural region of CVB5 genotypes B and D with the other EV-Bs, revealing eight recombination lineages. Our study showed the molecular evolution and phylogeography of CVB5 that could provide valuable information for disease prevention. |
| first_indexed | 2024-03-09T15:45:05Z |
| format | Article |
| id | doaj.art-bab7566c163c47f5be692d4fdac93f8b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T15:45:05Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-bab7566c163c47f5be692d4fdac93f8b2023-11-24T18:37:57ZengMDPI AGViruses1999-49152022-11-011412269310.3390/v14122693Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in ChinaYun He0Haiyan Wei1Leilei Wei2Huan Fan3Dongmei Yan4Hua Zhao5Shuangli Zhu6Tianjiao Ji7Jinbo Xiao8Huanhuan Lu9Wenhui Wang10Qin Guo11Qian Yang12Weijia Xing13Yong Zhang14National Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaHenan Center for Disease Control and Prevention, Zhengzhou 450016, ChinaJilin Center for Disease Control and Prevention, Jilin Institute of Public Health, Changchun 130062, ChinaJiangsu Center for Disease Control and Prevention, Nanjing 210009, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaChongqing Center for Disease Control and Prevention, Chongqing 400042, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaSchool of Public Health and Management, Shandong First Medical University, Shandong Academy of Medical Sciences, 6699 Qindao Road, Jinan 250117, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaSchool of Public Health and Management, Shandong First Medical University, Shandong Academy of Medical Sciences, 6699 Qindao Road, Jinan 250117, ChinaNational Polio Laboratory, WHO WPRO Regional Polio Reference Laboratory, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaCoxsackievirus B5 (CVB5) is an important enterovirus B species (EV-Bs) type. We used the full-length genomic sequences of 53 viral sequences from the national hand, foot, and mouth disease surveillance network in the Chinese mainland (2001–2021). Among them, 69 entire <i>VP1</i> coding region nucleotide sequences were used for CVB5 genotyping and genetic evolution analysis. Phylogenetic analysis based on a data set of 448 complete VP1 sequences showed that CVB5 could be divided into four genotypes (A-D) worldwide. Sequences from this study belonged to genotypes B and D, which dominated transmission in the Chinese mainland. Two transmission lineages of CVB5 have been discovered in the Chinese mainland, lineage 2 was predominant. Markov chain Monte Carlo analysis indicated that the tMRCA of CVB5 in the Chinese mainland could be traced to 1955, while the global trend could be traced to 1862, 93 years earlier than China. The evolution rate of CVB5 was higher in the Chinese mainland than worldwide. The spatiotemporal dynamics analysis of CVB5 assessed that virus transportation events were relatively active in Central, Northeast, North and Northwest China. Recombination analysis revealed frequent intertypic recombination in the non-structural region of CVB5 genotypes B and D with the other EV-Bs, revealing eight recombination lineages. Our study showed the molecular evolution and phylogeography of CVB5 that could provide valuable information for disease prevention.https://www.mdpi.com/1999-4915/14/12/2693coxsackievirus B5genotypingevolutionary dynamicsrecombination analysisgenetic diversityhand |
| spellingShingle | Yun He Haiyan Wei Leilei Wei Huan Fan Dongmei Yan Hua Zhao Shuangli Zhu Tianjiao Ji Jinbo Xiao Huanhuan Lu Wenhui Wang Qin Guo Qian Yang Weijia Xing Yong Zhang Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China Viruses coxsackievirus B5 genotyping evolutionary dynamics recombination analysis genetic diversity hand |
| title | Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China |
| title_full | Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China |
| title_fullStr | Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China |
| title_full_unstemmed | Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China |
| title_short | Molecular Epidemiology Reveals the Co-Circulation of Two Genotypes of Coxsackievirus B5 in China |
| title_sort | molecular epidemiology reveals the co circulation of two genotypes of coxsackievirus b5 in china |
| topic | coxsackievirus B5 genotyping evolutionary dynamics recombination analysis genetic diversity hand |
| url | https://www.mdpi.com/1999-4915/14/12/2693 |
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