Optimization of fermentation conditions for protease production from Bacillus subtilis
In the paper, the fermentation conditions and fermentation medium of bacillus subtilis protease production were optimized to improve the outcome of the protease produced from bacillus subtilis to provide premium raw materials for the subsequent preparation of chitin from crab shell. The optimal ferm...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2023/04/bioconf_icbb2023_01006.pdf |
| _version_ | 1797829439715803136 |
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| author | Jingying Chen Yan Gu |
| author_facet | Jingying Chen Yan Gu |
| author_sort | Jingying Chen |
| collection | DOAJ |
| description | In the paper, the fermentation conditions and fermentation medium of bacillus subtilis protease production were optimized to improve the outcome of the protease produced from bacillus subtilis to provide premium raw materials for the subsequent preparation of chitin from crab shell. The optimal fermentation conditions are temperature at 39°C, pH value of 7.5, fermentation time of 60h, inoculum size of 5%, filling volume of 80/250mL, and rotational speed at 180r/min; the optimal medium component ratio is 1.25% for starch mass concentration, 1% for yeast paste mass concentration, and 0.3% for sodium dihydrogen phosphate mass concentration. The activity of protease produced from bacillus subtilis GC021 is 174.87U/mL under the optimal fermentation conditions and at the optimal medium component ratio. |
| first_indexed | 2024-04-09T13:20:16Z |
| format | Article |
| id | doaj.art-bab7abcd16b2403db73b9045ab83ebc1 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-09T13:20:16Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-bab7abcd16b2403db73b9045ab83ebc12023-05-11T09:08:11ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01590100610.1051/bioconf/20235901006bioconf_icbb2023_01006Optimization of fermentation conditions for protease production from Bacillus subtilisJingying Chen0Yan Gu1Food Engineering Department, Maanshan Teacher’s CollegeFood Engineering Department, Maanshan Teacher’s CollegeIn the paper, the fermentation conditions and fermentation medium of bacillus subtilis protease production were optimized to improve the outcome of the protease produced from bacillus subtilis to provide premium raw materials for the subsequent preparation of chitin from crab shell. The optimal fermentation conditions are temperature at 39°C, pH value of 7.5, fermentation time of 60h, inoculum size of 5%, filling volume of 80/250mL, and rotational speed at 180r/min; the optimal medium component ratio is 1.25% for starch mass concentration, 1% for yeast paste mass concentration, and 0.3% for sodium dihydrogen phosphate mass concentration. The activity of protease produced from bacillus subtilis GC021 is 174.87U/mL under the optimal fermentation conditions and at the optimal medium component ratio.https://www.bio-conferences.org/articles/bioconf/pdf/2023/04/bioconf_icbb2023_01006.pdf |
| spellingShingle | Jingying Chen Yan Gu Optimization of fermentation conditions for protease production from Bacillus subtilis BIO Web of Conferences |
| title | Optimization of fermentation conditions for protease production from Bacillus subtilis |
| title_full | Optimization of fermentation conditions for protease production from Bacillus subtilis |
| title_fullStr | Optimization of fermentation conditions for protease production from Bacillus subtilis |
| title_full_unstemmed | Optimization of fermentation conditions for protease production from Bacillus subtilis |
| title_short | Optimization of fermentation conditions for protease production from Bacillus subtilis |
| title_sort | optimization of fermentation conditions for protease production from bacillus subtilis |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2023/04/bioconf_icbb2023_01006.pdf |
| work_keys_str_mv | AT jingyingchen optimizationoffermentationconditionsforproteaseproductionfrombacillussubtilis AT yangu optimizationoffermentationconditionsforproteaseproductionfrombacillussubtilis |