The Plasma Membrane H<sup>+</sup> ATPase CsPMA2 Regulates Lipid Droplet Formation, Appressorial Development and Virulence in <i>Colletotrichum siamense</i>
Plasma membrane H<sup>+</sup>-ATPases (PMAs) play an important role in the pathogenicity of pathogenic fungi. Lipid droplets are important storage sites for neutral lipids in fungal conidia and hyphae and can be used by plant pathogenic fungi for infection. However, the relationship betw...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/24/17337 |
| Summary: | Plasma membrane H<sup>+</sup>-ATPases (PMAs) play an important role in the pathogenicity of pathogenic fungi. Lipid droplets are important storage sites for neutral lipids in fungal conidia and hyphae and can be used by plant pathogenic fungi for infection. However, the relationship between plasma membrane H<sup>+</sup>-ATPase, lipid droplets and virulence remains unclear. Here, we characterized a plasma membrane H<sup>+</sup>-ATPase, CsPMA2, that plays a key role in lipid droplet formation, appresorial development and virulence in <i>C. siamense</i>. Deletion of <i>CsPMA2</i> impaired <i>C. siamense</i> conidial size, conidial germination, appressorial development and virulence but did not affect hyphal growth. Δ<i>CsPMA2</i> increased the sensitivity of <i>C. siamense</i> to phytic acid and oxalic acid. CsPMA2 was localized to lipids on the plasma membrane and intracellular membrane. Deletion of <i>CsPMA2</i> significantly inhibited the accumulation of lipid droplets and significantly affected the contents of some species of lipids, including 12 species with decreased lipid contents and 3 species with increased lipid contents. Furthermore, low pH can inhibit <i>CsPMA2</i> expression and lipid droplet accumulation. Overall, our data revealed that the plasma membrane H<sup>+</sup>-ATPase CsPMA2 is involved in the regulation of lipid droplet formation and affects appressorial development and virulence in <i>C. siamense</i>. |
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| ISSN: | 1661-6596 1422-0067 |