Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light
Light pollution by artificial light, might accelerate retinal diseases and circadian asynchrony. The excess of light exposure is a growing problem in societies, so studies on the consequences of long-term exposure to low levels of light are needed to determine the effects on vision. The possibility...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2019.00139/full |
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| author | Maria M. Benedetto Maria M. Benedetto Maria A. Contin Maria A. Contin |
| author_facet | Maria M. Benedetto Maria M. Benedetto Maria A. Contin Maria A. Contin |
| author_sort | Maria M. Benedetto |
| collection | DOAJ |
| description | Light pollution by artificial light, might accelerate retinal diseases and circadian asynchrony. The excess of light exposure is a growing problem in societies, so studies on the consequences of long-term exposure to low levels of light are needed to determine the effects on vision. The possibility to understand the molecular mechanisms of light damage will contribute to the knowledge about visual disorders related to defects in the phototransduction. Several animal models have been used to study retinal degeneration (RD) by light; however, some important aspects remain to be established. Previously, we demonstrated that cool white treatment of 200 lux light-emitting diode (LED) induces retinal transformation with rods and cones cell death and significant changes in opsin expression in the inner nuclear layer (INL) and ganglion cell layer (GCL). Therefore, to further develop describing the molecular pathways of RD, we have examined here the oxidative stress and the fatty acid composition in rat retinas maintained at constant light. We demonstrated the existence of oxidative reactions after 5 days in outer nuclear layer (ONL), corresponding to classical photoreceptors; catalase (CAT) enzyme activity did not show significant differences in all times studied and the fatty acid study showed that docosahexaenoic acid decreased after 4 days. Remarkably, the docosahexaenoic acid diminution showed a correlation with the rise in stearic acid indicating a possible association between them. We assumed that the reduction in docosahexaenoic acid may be affected by the oxidative stress in photoreceptors outer segment which in turn affects the stearic acid composition with consequences in the membrane properties. All these miss-regulation affects the photoreceptor survival through unknown mechanisms involved. We consider that oxidative stress might be one of the pathways implicated in RD promoted by light. |
| first_indexed | 2024-12-21T18:32:55Z |
| format | Article |
| id | doaj.art-bac194c28e53451ba95849828dc96e6c |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-21T18:32:55Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-bac194c28e53451ba95849828dc96e6c2022-12-21T18:54:13ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022019-04-011310.3389/fncel.2019.00139441593Oxidative Stress in Retinal Degeneration Promoted by Constant LED LightMaria M. Benedetto0Maria M. Benedetto1Maria A. Contin2Maria A. Contin3Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, ArgentinaCentro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET, Universidad Nacional de Córdoba, Córdoba, ArgentinaDepartamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, ArgentinaCentro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET, Universidad Nacional de Córdoba, Córdoba, ArgentinaLight pollution by artificial light, might accelerate retinal diseases and circadian asynchrony. The excess of light exposure is a growing problem in societies, so studies on the consequences of long-term exposure to low levels of light are needed to determine the effects on vision. The possibility to understand the molecular mechanisms of light damage will contribute to the knowledge about visual disorders related to defects in the phototransduction. Several animal models have been used to study retinal degeneration (RD) by light; however, some important aspects remain to be established. Previously, we demonstrated that cool white treatment of 200 lux light-emitting diode (LED) induces retinal transformation with rods and cones cell death and significant changes in opsin expression in the inner nuclear layer (INL) and ganglion cell layer (GCL). Therefore, to further develop describing the molecular pathways of RD, we have examined here the oxidative stress and the fatty acid composition in rat retinas maintained at constant light. We demonstrated the existence of oxidative reactions after 5 days in outer nuclear layer (ONL), corresponding to classical photoreceptors; catalase (CAT) enzyme activity did not show significant differences in all times studied and the fatty acid study showed that docosahexaenoic acid decreased after 4 days. Remarkably, the docosahexaenoic acid diminution showed a correlation with the rise in stearic acid indicating a possible association between them. We assumed that the reduction in docosahexaenoic acid may be affected by the oxidative stress in photoreceptors outer segment which in turn affects the stearic acid composition with consequences in the membrane properties. All these miss-regulation affects the photoreceptor survival through unknown mechanisms involved. We consider that oxidative stress might be one of the pathways implicated in RD promoted by light.https://www.frontiersin.org/article/10.3389/fncel.2019.00139/fullretinal light damageLED lightoxidative stressfatty acidelectroretinogram |
| spellingShingle | Maria M. Benedetto Maria M. Benedetto Maria A. Contin Maria A. Contin Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light Frontiers in Cellular Neuroscience retinal light damage LED light oxidative stress fatty acid electroretinogram |
| title | Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light |
| title_full | Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light |
| title_fullStr | Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light |
| title_full_unstemmed | Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light |
| title_short | Oxidative Stress in Retinal Degeneration Promoted by Constant LED Light |
| title_sort | oxidative stress in retinal degeneration promoted by constant led light |
| topic | retinal light damage LED light oxidative stress fatty acid electroretinogram |
| url | https://www.frontiersin.org/article/10.3389/fncel.2019.00139/full |
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