A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent
Biocompatibility testing of materials is carried out in 2D cell cultures or animal models despite serious limitations. 3D skin equivalents are advanced in vitro models for human skin. Silicone has been shown to be noncytotoxic but capable of eliciting an immune response. Our aim was to (1) establish...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/224 |
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| author | Rima Nuwayhid Torsten Schulz Frank Siemers Jeannine Schreiter Philipp Kobbe Gunther Hofmann Stefan Langer Olga Kurow |
| author_facet | Rima Nuwayhid Torsten Schulz Frank Siemers Jeannine Schreiter Philipp Kobbe Gunther Hofmann Stefan Langer Olga Kurow |
| author_sort | Rima Nuwayhid |
| collection | DOAJ |
| description | Biocompatibility testing of materials is carried out in 2D cell cultures or animal models despite serious limitations. 3D skin equivalents are advanced in vitro models for human skin. Silicone has been shown to be noncytotoxic but capable of eliciting an immune response. Our aim was to (1) establish a 3D skin equivalent to (2) assess the proinflammatory properties of silicone. We developed a coculture of keratinocytes and fibroblasts resulting in a 3D skin equivalent with an implant using samples from a breast implant. Samples with and without the silicone implant were studied histologically and immunohistochemically in comparison to native human skin samples. Cytotoxicity was assessed via LDH-assay, and cytokine response was assessed via ELISA. Histologically, our 3D skin equivalents had a four-layered epidermal and a dermal component. The presence of tight junctions was demonstrated in immunofluorescence. The only difference in 3D skin equivalents with implants was an epidermal thinning. Implanting the silicone samples did not cause more cell death, however, an inflammatory cytokine response was triggered. We were able to establish an organotypical 3D skin equivalent with an implant, which can be utilised for studies on biocompatibility of materials. This first integration of silicone into a 3D skin equivalent confirmed previous findings on silicone being non-cell-toxic but capable of exerting a proinflammatory effect. |
| first_indexed | 2024-03-08T09:57:10Z |
| format | Article |
| id | doaj.art-bac2551037e345bf9d692e421a714528 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:57:10Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-bac2551037e345bf9d692e421a7145282024-01-29T13:47:58ZengMDPI AGBiomedicines2227-90592024-01-0112122410.3390/biomedicines12010224A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin EquivalentRima Nuwayhid0Torsten Schulz1Frank Siemers2Jeannine Schreiter3Philipp Kobbe4Gunther Hofmann5Stefan Langer6Olga Kurow7Department of Orthopaedic, Trauma and Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Orthopaedic, Trauma and Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Plastic, Hand Surgery and Burn Care, BG Klinikum Bergmannstrost, 06112 Halle, GermanyKlinik am Rosental GmbH, 04105 Leipzig, GermanyDepartment of Trauma and Reconstructive Surgery, Martin-Luther-University Halle-Wittenberg, 06120 Halle, GermanyDepartment of Trauma, Plastic and Reconstructive Surgery, University Hospital Jena, 07747 Jena, GermanyDepartment of Orthopaedic, Trauma and Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Orthopaedic, Trauma and Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyBiocompatibility testing of materials is carried out in 2D cell cultures or animal models despite serious limitations. 3D skin equivalents are advanced in vitro models for human skin. Silicone has been shown to be noncytotoxic but capable of eliciting an immune response. Our aim was to (1) establish a 3D skin equivalent to (2) assess the proinflammatory properties of silicone. We developed a coculture of keratinocytes and fibroblasts resulting in a 3D skin equivalent with an implant using samples from a breast implant. Samples with and without the silicone implant were studied histologically and immunohistochemically in comparison to native human skin samples. Cytotoxicity was assessed via LDH-assay, and cytokine response was assessed via ELISA. Histologically, our 3D skin equivalents had a four-layered epidermal and a dermal component. The presence of tight junctions was demonstrated in immunofluorescence. The only difference in 3D skin equivalents with implants was an epidermal thinning. Implanting the silicone samples did not cause more cell death, however, an inflammatory cytokine response was triggered. We were able to establish an organotypical 3D skin equivalent with an implant, which can be utilised for studies on biocompatibility of materials. This first integration of silicone into a 3D skin equivalent confirmed previous findings on silicone being non-cell-toxic but capable of exerting a proinflammatory effect.https://www.mdpi.com/2227-9059/12/1/224tissue engineering3D skin equivalent3D skin modelsiliconebreast implantsbiocompatibility |
| spellingShingle | Rima Nuwayhid Torsten Schulz Frank Siemers Jeannine Schreiter Philipp Kobbe Gunther Hofmann Stefan Langer Olga Kurow A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent Biomedicines tissue engineering 3D skin equivalent 3D skin model silicone breast implants biocompatibility |
| title | A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent |
| title_full | A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent |
| title_fullStr | A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent |
| title_full_unstemmed | A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent |
| title_short | A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent |
| title_sort | platform for testing the biocompatibility of implants silicone induces a proinflammatory response in a 3d skin equivalent |
| topic | tissue engineering 3D skin equivalent 3D skin model silicone breast implants biocompatibility |
| url | https://www.mdpi.com/2227-9059/12/1/224 |
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