Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements
IntroductionInduced modification of plant gene expression is of both fundamental and applied importance. Cis-acting regulatory elements (CREs) are major determinants of the spatiotemporal strength of gene expression. Yet, there are few examples where induced genetic variation in predetermined CREs h...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1372049/full |
| _version_ | 1797289825550729216 |
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| author | Claus Krogh Madsen Charles Alistair Brearley Jesper Harholt Henrik Brinch-Pedersen |
| author_facet | Claus Krogh Madsen Charles Alistair Brearley Jesper Harholt Henrik Brinch-Pedersen |
| author_sort | Claus Krogh Madsen |
| collection | DOAJ |
| description | IntroductionInduced modification of plant gene expression is of both fundamental and applied importance. Cis-acting regulatory elements (CREs) are major determinants of the spatiotemporal strength of gene expression. Yet, there are few examples where induced genetic variation in predetermined CREs has been exploited to improve or investigate crop plants. MethodsThe digital PCR based FIND-IT technology was applied to discover barley mutants with CRE variants in the promoter of the nutritional important barley grain phytase (PAPhy_a) gene. Results and discussionMutants with higher or lower gene expression and ultimately higher or lower mature grain phytase activity (MGPA), respectively, were discovered. Field trials and inositol phosphate profiling during germination showed that PAPhy_a does not influence agronomic performance under the trial conditions but it does shorten the lag time of phosphate mobilization during germination. Higher endogenous MGPA is an improvement of grain quality for feed use as it improves the phosphate bioavailability for monogastric animals. Moreover, as the targeted CRE motifs of the PAPhy_a promoter are shared with a range of seed expressed genes like key cereal and legume storage genes, the current results demonstrates a concept for modulating individual gene expression levels of a range of seed genes. |
| first_indexed | 2024-03-07T19:10:41Z |
| format | Article |
| id | doaj.art-bac37e90f31b4f1a97dd1521100c782e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-07T19:10:41Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-bac37e90f31b4f1a97dd1521100c782e2024-03-01T04:31:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-03-011510.3389/fpls.2024.13720491372049Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elementsClaus Krogh Madsen0Charles Alistair Brearley1Jesper Harholt2Henrik Brinch-Pedersen3Department of Agroecology, Aarhus University, Slagelse, DenmarkSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United KingdomCarlsberg Research Laboratory, J. C. Jacobsens Gade 4, Copenhagen, DenmarkDepartment of Agroecology, Aarhus University, Slagelse, DenmarkIntroductionInduced modification of plant gene expression is of both fundamental and applied importance. Cis-acting regulatory elements (CREs) are major determinants of the spatiotemporal strength of gene expression. Yet, there are few examples where induced genetic variation in predetermined CREs has been exploited to improve or investigate crop plants. MethodsThe digital PCR based FIND-IT technology was applied to discover barley mutants with CRE variants in the promoter of the nutritional important barley grain phytase (PAPhy_a) gene. Results and discussionMutants with higher or lower gene expression and ultimately higher or lower mature grain phytase activity (MGPA), respectively, were discovered. Field trials and inositol phosphate profiling during germination showed that PAPhy_a does not influence agronomic performance under the trial conditions but it does shorten the lag time of phosphate mobilization during germination. Higher endogenous MGPA is an improvement of grain quality for feed use as it improves the phosphate bioavailability for monogastric animals. Moreover, as the targeted CRE motifs of the PAPhy_a promoter are shared with a range of seed expressed genes like key cereal and legume storage genes, the current results demonstrates a concept for modulating individual gene expression levels of a range of seed genes.https://www.frontiersin.org/articles/10.3389/fpls.2024.1372049/fullCREpromoterphytasebarleyphytateFIND-IT |
| spellingShingle | Claus Krogh Madsen Charles Alistair Brearley Jesper Harholt Henrik Brinch-Pedersen Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements Frontiers in Plant Science CRE promoter phytase barley phytate FIND-IT |
| title | Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements |
| title_full | Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements |
| title_fullStr | Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements |
| title_full_unstemmed | Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements |
| title_short | Optimized barley phytase gene expression by focused FIND-IT screening for mutations in cis-acting regulatory elements |
| title_sort | optimized barley phytase gene expression by focused find it screening for mutations in cis acting regulatory elements |
| topic | CRE promoter phytase barley phytate FIND-IT |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1372049/full |
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