Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to charac...
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BMC
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005&lng=en&tlng=en |
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| author | Kurt Buchegger Carmen Ili Ismael Riquelme Pablo Letelier Alejandro H. Corvalán Priscilla Brebi Tim Hui-Ming Huang Juan Carlos Roa |
| author_facet | Kurt Buchegger Carmen Ili Ismael Riquelme Pablo Letelier Alejandro H. Corvalán Priscilla Brebi Tim Hui-Ming Huang Juan Carlos Roa |
| author_sort | Kurt Buchegger |
| collection | DOAJ |
| description | BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERa(+) tumors showed higher methylation intensity than ERa(-). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell migration, wound healing and invasion in vitro. However, RPRM overexpression did not alter cell viability, phosphatidylserine (PS) translocation or G2/M cell cycle transition. CONCLUSION: Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line. |
| first_indexed | 2024-04-12T01:32:13Z |
| format | Article |
| id | doaj.art-bac59d27ff9243939a55891e1b8d58fa |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-04-12T01:32:13Z |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-bac59d27ff9243939a55891e1b8d58fa2022-12-22T03:53:27ZengBMCBiological Research0716-976049011010.1186/s40659-016-0066-7S0716-97602016000100005Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell lineKurt Buchegger0Carmen Ili1Ismael Riquelme2Pablo Letelier3Alejandro H. Corvalán4Priscilla Brebi5Tim Hui-Ming Huang6Juan Carlos Roa7Universidad de La FronteraUniversidad de La FronteraUniversidad de La FronteraUniversidad Católica de TemucoPontificia Universidad Catolica de ChileUniversidad de La FronteraUniversity of Texas Health Science CenterPontificia Universidad Católica de ChileBACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERa(+) tumors showed higher methylation intensity than ERa(-). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell migration, wound healing and invasion in vitro. However, RPRM overexpression did not alter cell viability, phosphatidylserine (PS) translocation or G2/M cell cycle transition. CONCLUSION: Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005&lng=en&tlng=enReprimoMDA-MB-231MigrationInvasion |
| spellingShingle | Kurt Buchegger Carmen Ili Ismael Riquelme Pablo Letelier Alejandro H. Corvalán Priscilla Brebi Tim Hui-Ming Huang Juan Carlos Roa Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line Biological Research Reprimo MDA-MB-231 Migration Invasion |
| title | Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line |
| title_full | Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line |
| title_fullStr | Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line |
| title_full_unstemmed | Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line |
| title_short | Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line |
| title_sort | reprimo as a modulator of cell migration and invasion in the mda mb 231 breast cancer cell line |
| topic | Reprimo MDA-MB-231 Migration Invasion |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005&lng=en&tlng=en |
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