First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia
Circulation of a multi-resistance clone of bacteria associated with genetic elements in diseased animals constitutes a global public health problem. Our study focused on the characterization of the support of ESBL in cefotaxime resistant <i>E. coli</i> (CTX<sup>R</sup>) isola...
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MDPI AG
2021-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/6/670 |
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| author | Ahlem Jouini Amira Klibi Imen Elarbi Meriem Ben Chaabene Safa Hamrouni Oussema Souiai Mariem Hanachi Abdeljelil Ghram Abderrazak Maaroufi |
| author_facet | Ahlem Jouini Amira Klibi Imen Elarbi Meriem Ben Chaabene Safa Hamrouni Oussema Souiai Mariem Hanachi Abdeljelil Ghram Abderrazak Maaroufi |
| author_sort | Ahlem Jouini |
| collection | DOAJ |
| description | Circulation of a multi-resistance clone of bacteria associated with genetic elements in diseased animals constitutes a global public health problem. Our study focused on the characterization of the support of ESBL in cefotaxime resistant <i>E. coli</i> (CTX<sup>R</sup>) isolates recovered from poultry with diarrhea, analysis of their clonal lineage, and virulence-associated genes. The study was carried out on 130 samples of chickens with diarrhea, collected in 2015 from poultry farms in Tunisia. Isolates of 20 CTX<sup>R</sup> <i>E. coli</i> strains were identified as ESBL and AmpC β- lactamase producers. The following β-lactamase genes (number of isolates) were detected: <i>bla</i><sub>CTX-M-15</sub>+ <i>bla</i><sub>OXA1</sub> (4), <i>bla</i><sub>CTX-M-15</sub> + <i>bla</i><sub>OXA1</sub> + <i>bla</i><sub>TEM-1b</sub> (2), <i>bla</i><sub>CTX-M-1</sub> + <i>bla</i><sub>TEM-1b</sub> (9), <i>bla</i><sub>CTX-M-1</sub> (2), <i>bla</i><sub>CMY2</sub> + <i>bla</i><sub>TEM-1b</sub> (3). Six <i>E. coli</i> harboring <i>bla</i><sub>CTXM-15</sub> were allocated to ST131-B2-O25b-; six and three <i>bla</i><sub>CTX-M-1</sub> were grouped in ST155, ST10, and ST58, respectively, related to the phylogroup D and A. The <i>qnrB</i> gene, the variant <i>aac(6′)-Ib-cr,</i> and the class 1 integrons with different gene cassettes, were detected amongst our 20 isolated strains, which were classified as ExPEC and aEPEC. Our findings highlighted the emergence of the human pandemic ST131-CTX-M-15-O25-B2 clone and the high risk of such clonal lineage strains in diarrheic poultry, in Tunisia, which could constitute a risk of their transfer to healthy animals and humans. |
| first_indexed | 2024-03-10T10:44:12Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:44:12Z |
| publishDate | 2021-06-01 |
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| series | Antibiotics |
| spelling | doaj.art-bacaa37bac384a56a1083f5824600d4c2023-11-21T22:44:46ZengMDPI AGAntibiotics2079-63822021-06-0110667010.3390/antibiotics10060670First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in TunisiaAhlem Jouini0Amira Klibi1Imen Elarbi2Meriem Ben Chaabene3Safa Hamrouni4Oussema Souiai5Mariem Hanachi6Abdeljelil Ghram7Abderrazak Maaroufi8Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Bioinformatics, Biomathematics and Biostatistics-LR16IPT09, Institute Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 2092, TunisiaLaboratory of Bioinformatics, Biomathematics and Biostatistics-LR16IPT09, Institute Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 2092, TunisiaLaboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, TunisiaCirculation of a multi-resistance clone of bacteria associated with genetic elements in diseased animals constitutes a global public health problem. Our study focused on the characterization of the support of ESBL in cefotaxime resistant <i>E. coli</i> (CTX<sup>R</sup>) isolates recovered from poultry with diarrhea, analysis of their clonal lineage, and virulence-associated genes. The study was carried out on 130 samples of chickens with diarrhea, collected in 2015 from poultry farms in Tunisia. Isolates of 20 CTX<sup>R</sup> <i>E. coli</i> strains were identified as ESBL and AmpC β- lactamase producers. The following β-lactamase genes (number of isolates) were detected: <i>bla</i><sub>CTX-M-15</sub>+ <i>bla</i><sub>OXA1</sub> (4), <i>bla</i><sub>CTX-M-15</sub> + <i>bla</i><sub>OXA1</sub> + <i>bla</i><sub>TEM-1b</sub> (2), <i>bla</i><sub>CTX-M-1</sub> + <i>bla</i><sub>TEM-1b</sub> (9), <i>bla</i><sub>CTX-M-1</sub> (2), <i>bla</i><sub>CMY2</sub> + <i>bla</i><sub>TEM-1b</sub> (3). Six <i>E. coli</i> harboring <i>bla</i><sub>CTXM-15</sub> were allocated to ST131-B2-O25b-; six and three <i>bla</i><sub>CTX-M-1</sub> were grouped in ST155, ST10, and ST58, respectively, related to the phylogroup D and A. The <i>qnrB</i> gene, the variant <i>aac(6′)-Ib-cr,</i> and the class 1 integrons with different gene cassettes, were detected amongst our 20 isolated strains, which were classified as ExPEC and aEPEC. Our findings highlighted the emergence of the human pandemic ST131-CTX-M-15-O25-B2 clone and the high risk of such clonal lineage strains in diarrheic poultry, in Tunisia, which could constitute a risk of their transfer to healthy animals and humans.https://www.mdpi.com/2079-6382/10/6/670ESBLST131clonal lineagespAmpCdiarrheic poultrypathogenic bacteria |
| spellingShingle | Ahlem Jouini Amira Klibi Imen Elarbi Meriem Ben Chaabene Safa Hamrouni Oussema Souiai Mariem Hanachi Abdeljelil Ghram Abderrazak Maaroufi First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia Antibiotics ESBL ST131 clonal lineages pAmpC diarrheic poultry pathogenic bacteria |
| title | First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia |
| title_full | First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia |
| title_fullStr | First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia |
| title_full_unstemmed | First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia |
| title_short | First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing <i>E. coli</i> Isolates from Diarrheic Poultry in Tunisia |
| title_sort | first detection of human st131 ctx m 15 o25 b2 clone and high risk clonal lineages of esbl pampc producing i e coli i isolates from diarrheic poultry in tunisia |
| topic | ESBL ST131 clonal lineages pAmpC diarrheic poultry pathogenic bacteria |
| url | https://www.mdpi.com/2079-6382/10/6/670 |
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