Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice
In this study, the in vivo effects of Antarctic krill oil (AKO) on cartilage degeneration in destabilization of the medial meniscus (DMM) mouse were investigated. Results showed that AKO improved the cartilage structure, inhibited chondrocyte hypertrophic differentiation and inhibited abnormal apopt...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-07-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646461830224X |
| _version_ | 1818920088817893376 |
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| author | Kai Wang Lihua Han Yujie Zhu Yanjun Liu Jingfeng Wang Changhu Xue |
| author_facet | Kai Wang Lihua Han Yujie Zhu Yanjun Liu Jingfeng Wang Changhu Xue |
| author_sort | Kai Wang |
| collection | DOAJ |
| description | In this study, the in vivo effects of Antarctic krill oil (AKO) on cartilage degeneration in destabilization of the medial meniscus (DMM) mouse were investigated. Results showed that AKO improved the cartilage structure, inhibited chondrocyte hypertrophic differentiation and inhibited abnormal apoptosis in articular cartilage. Autophagy is a self-protective metabolic process required for maintaining cartilage homeostasis. We observed that expression of mTOR were reduced, which was consist with high PPAR-γ levels. The expression of key genes related to autophagy, including LC-3B, Beclin-1, ATG-5, and BNIP-3 were significantly enhanced after treatment with AKO. In addition, we observed that AKO suppressed the expression of key genes in p53-dependent mitochondrial apoptotic pathway, such as p53, Bax, Bid, cytochrome c, caspase-9, caspase-3 and enhanced the expression of anti-apoptotic genes, including Bcl-2 and Bcl-xl. These findings provided a new perspective for the effect of AKO on OA, which associated with the regulation of autophagy and apoptosis. |
| first_indexed | 2024-12-20T01:16:12Z |
| format | Article |
| id | doaj.art-baccf8de6c874533a675c5d5d2af6267 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T01:16:12Z |
| publishDate | 2018-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-baccf8de6c874533a675c5d5d2af62672022-12-21T19:58:34ZengElsevierJournal of Functional Foods1756-46462018-07-0146413422Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis miceKai Wang0Lihua Han1Yujie Zhu2Yanjun Liu3Jingfeng Wang4Changhu Xue5College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, ChinaCorresponding authors at: College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road, Qingdao, Shandong Province 266003, China.; College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, ChinaCorresponding authors at: College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road, Qingdao, Shandong Province 266003, China.; College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, ChinaIn this study, the in vivo effects of Antarctic krill oil (AKO) on cartilage degeneration in destabilization of the medial meniscus (DMM) mouse were investigated. Results showed that AKO improved the cartilage structure, inhibited chondrocyte hypertrophic differentiation and inhibited abnormal apoptosis in articular cartilage. Autophagy is a self-protective metabolic process required for maintaining cartilage homeostasis. We observed that expression of mTOR were reduced, which was consist with high PPAR-γ levels. The expression of key genes related to autophagy, including LC-3B, Beclin-1, ATG-5, and BNIP-3 were significantly enhanced after treatment with AKO. In addition, we observed that AKO suppressed the expression of key genes in p53-dependent mitochondrial apoptotic pathway, such as p53, Bax, Bid, cytochrome c, caspase-9, caspase-3 and enhanced the expression of anti-apoptotic genes, including Bcl-2 and Bcl-xl. These findings provided a new perspective for the effect of AKO on OA, which associated with the regulation of autophagy and apoptosis.http://www.sciencedirect.com/science/article/pii/S175646461830224XAntarctic krill oilOsteoarthritisCartilage degenerationAutophagyApoptosis |
| spellingShingle | Kai Wang Lihua Han Yujie Zhu Yanjun Liu Jingfeng Wang Changhu Xue Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice Journal of Functional Foods Antarctic krill oil Osteoarthritis Cartilage degeneration Autophagy Apoptosis |
| title | Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice |
| title_full | Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice |
| title_fullStr | Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice |
| title_full_unstemmed | Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice |
| title_short | Antarctic Krill Oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice |
| title_sort | antarctic krill oil improves articular cartilage degeneration via activating chondrocyte autophagy and inhibiting apoptosis in osteoarthritis mice |
| topic | Antarctic krill oil Osteoarthritis Cartilage degeneration Autophagy Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S175646461830224X |
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