The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators
Auxin plays a dual role in growth regulation and, depending on the tissue and concentration of the hormone, it can either promote or inhibit division and expansion processes in plants. Recent studies have revealed that, beyond transcriptional reprogramming, alternative auxin-controlled mechanisms re...
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| Format: | Article |
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MDPI AG
2021-07-01
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| author | Natalia Nikonorova Evan Murphy Cassio Flavio Fonseca de Lima Shanshuo Zhu Brigitte van de Cotte Lam Dai Vu Daria Balcerowicz Lanxin Li Xiangpei Kong Gieljan De Rop Tom Beeckman Jiří Friml Kris Vissenberg Peter C. Morris Zhaojun Ding Ive De Smet |
| author_facet | Natalia Nikonorova Evan Murphy Cassio Flavio Fonseca de Lima Shanshuo Zhu Brigitte van de Cotte Lam Dai Vu Daria Balcerowicz Lanxin Li Xiangpei Kong Gieljan De Rop Tom Beeckman Jiří Friml Kris Vissenberg Peter C. Morris Zhaojun Ding Ive De Smet |
| author_sort | Natalia Nikonorova |
| collection | DOAJ |
| description | Auxin plays a dual role in growth regulation and, depending on the tissue and concentration of the hormone, it can either promote or inhibit division and expansion processes in plants. Recent studies have revealed that, beyond transcriptional reprogramming, alternative auxin-controlled mechanisms regulate root growth. Here, we explored the impact of different concentrations of the synthetic auxin NAA that establish growth-promoting and -repressing conditions on the root tip proteome and phosphoproteome, generating a unique resource. From the phosphoproteome data, we pinpointed (novel) growth regulators, such as the RALF34-THE1 module. Our results, together with previously published studies, suggest that auxin, H<sup>+</sup>-ATPases, cell wall modifications and cell wall sensing receptor-like kinases are tightly embedded in a pathway regulating cell elongation. Furthermore, our study assigned a novel role to MKK2 as a regulator of primary root growth and a (potential) regulator of auxin biosynthesis and signalling, and suggests the importance of the MKK2 Thr<sup>31</sup> phosphorylation site for growth regulation in the <i>Arabidopsis</i> root tip. |
| first_indexed | 2024-03-10T09:43:25Z |
| format | Article |
| id | doaj.art-bad01ad2921c41d68a4b3f40324e2124 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T09:43:25Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-bad01ad2921c41d68a4b3f40324e21242023-11-22T03:28:15ZengMDPI AGCells2073-44092021-07-01107166510.3390/cells10071665The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth RegulatorsNatalia Nikonorova0Evan Murphy1Cassio Flavio Fonseca de Lima2Shanshuo Zhu3Brigitte van de Cotte4Lam Dai Vu5Daria Balcerowicz6Lanxin Li7Xiangpei Kong8Gieljan De Rop9Tom Beeckman10Jiří Friml11Kris Vissenberg12Peter C. Morris13Zhaojun Ding14Ive De Smet15Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumDivision of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UKDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumIntegrated Molecular Plant Physiology Research, Biology Department, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumInstitute of Science and Technology (IST) Austria, 3400 Klosterneuburg, AustriaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumInstitute of Science and Technology (IST) Austria, 3400 Klosterneuburg, AustriaIntegrated Molecular Plant Physiology Research, Biology Department, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumInstitute for Life and Earth Sciences, School of Energy, Geosciences, Infrastructure and Society, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UKThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumAuxin plays a dual role in growth regulation and, depending on the tissue and concentration of the hormone, it can either promote or inhibit division and expansion processes in plants. Recent studies have revealed that, beyond transcriptional reprogramming, alternative auxin-controlled mechanisms regulate root growth. Here, we explored the impact of different concentrations of the synthetic auxin NAA that establish growth-promoting and -repressing conditions on the root tip proteome and phosphoproteome, generating a unique resource. From the phosphoproteome data, we pinpointed (novel) growth regulators, such as the RALF34-THE1 module. Our results, together with previously published studies, suggest that auxin, H<sup>+</sup>-ATPases, cell wall modifications and cell wall sensing receptor-like kinases are tightly embedded in a pathway regulating cell elongation. Furthermore, our study assigned a novel role to MKK2 as a regulator of primary root growth and a (potential) regulator of auxin biosynthesis and signalling, and suggests the importance of the MKK2 Thr<sup>31</sup> phosphorylation site for growth regulation in the <i>Arabidopsis</i> root tip.https://www.mdpi.com/2073-4409/10/7/1665primary root(phospho)proteomicsauxin(receptor) kinase |
| spellingShingle | Natalia Nikonorova Evan Murphy Cassio Flavio Fonseca de Lima Shanshuo Zhu Brigitte van de Cotte Lam Dai Vu Daria Balcerowicz Lanxin Li Xiangpei Kong Gieljan De Rop Tom Beeckman Jiří Friml Kris Vissenberg Peter C. Morris Zhaojun Ding Ive De Smet The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators Cells primary root (phospho)proteomics auxin (receptor) kinase |
| title | The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators |
| title_full | The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators |
| title_fullStr | The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators |
| title_full_unstemmed | The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators |
| title_short | The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators |
| title_sort | arabidopsis root tip phospho proteomes at growth promoting versus growth repressing conditions reveal novel root growth regulators |
| topic | primary root (phospho)proteomics auxin (receptor) kinase |
| url | https://www.mdpi.com/2073-4409/10/7/1665 |
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