Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference
Summary: The underlying mechanisms of how positive emotional valence (e.g., pleasure) causes preference of an associated context is poorly understood. Here, we show that activation of astrocytic μ-opioid receptor (MOR) drives conditioned place preference (CPP) by means of specific modulation of astr...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719308514 |
| _version_ | 1819080953694257152 |
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| author | Min-Ho Nam Kyung-Seok Han Jaekwang Lee Woojin Won Wuhyun Koh Jin Young Bae Junsung Woo Jayoung Kim Elliot Kwong Tae-Yong Choi Heejung Chun Seung Eun Lee Sang-Bum Kim Ki Duk Park Se-Young Choi Yong Chul Bae C. Justin Lee |
| author_facet | Min-Ho Nam Kyung-Seok Han Jaekwang Lee Woojin Won Wuhyun Koh Jin Young Bae Junsung Woo Jayoung Kim Elliot Kwong Tae-Yong Choi Heejung Chun Seung Eun Lee Sang-Bum Kim Ki Duk Park Se-Young Choi Yong Chul Bae C. Justin Lee |
| author_sort | Min-Ho Nam |
| collection | DOAJ |
| description | Summary: The underlying mechanisms of how positive emotional valence (e.g., pleasure) causes preference of an associated context is poorly understood. Here, we show that activation of astrocytic μ-opioid receptor (MOR) drives conditioned place preference (CPP) by means of specific modulation of astrocytic MOR, an exemplar endogenous Gi protein-coupled receptor (Gi-GPCR), in the CA1 hippocampus. Long-term potentiation (LTP) induced by a subthreshold stimulation with the activation of astrocytic MOR at the Schaffer collateral pathway accounts for the memory acquisition to induce CPP. This astrocytic MOR-mediated LTP induction is dependent on astrocytic glutamate released upon activation of the astrocytic MOR and the consequent activation of the presynaptic mGluR1. The astrocytic MOR-dependent LTP and CPP were recapitulated by a chemogenetic activation of astrocyte-specifically expressed Gi-DREADD hM4Di. Our study reveals that the transduction of inhibitory Gi-signaling into augmented excitatory synaptic transmission through astrocytic glutamate is critical for the acquisition of contextual memory for CPP. : Nam et al. demonstrate that activation of hippocampal astrocytic μ-opioid receptor causes glutamate release, which increases the release probability by neuronal presynaptic mGluR1 activation and potentiates synaptic plasticity at the SC-CA1 pathway. This enhanced synaptic transmission and synaptic plasticity account for the acquisition of memory associated with CPP. Keywords: astrocyte, hippocampus, μ-opioid receptor, conditioned place preference, long-term potentiation opioid |
| first_indexed | 2024-12-21T19:53:04Z |
| format | Article |
| id | doaj.art-bad3616ea2ef44b1b0a2ee9256c9f284 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T19:53:04Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-bad3616ea2ef44b1b0a2ee9256c9f2842022-12-21T18:52:10ZengElsevierCell Reports2211-12472019-07-0128511541166.e5Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place PreferenceMin-Ho Nam0Kyung-Seok Han1Jaekwang Lee2Woojin Won3Wuhyun Koh4Jin Young Bae5Junsung Woo6Jayoung Kim7Elliot Kwong8Tae-Yong Choi9Heejung Chun10Seung Eun Lee11Sang-Bum Kim12Ki Duk Park13Se-Young Choi14Yong Chul Bae15C. Justin Lee16Center for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; Department of Neuroscience, Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Korea; Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34126, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; Department of Neuroscience, Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Korea; Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34126, KoreaDepartment of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu 41940, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; Department of Neuroscience, Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaDepartment of Physiology and Dental Research Institute, Seoul National University School of Dentistry, Seoul 03080, KoreaCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34126, KoreaVirus Facility, Research Animal Resource Center, KIST, Seoul 02792, KoreaNew Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, KoreaDepartment of Neuroscience, Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Korea; Convergence Research Center for Diagnosis, Treatment and Care System of Dementia, KIST, Seoul 02792, KoreaDepartment of Physiology and Dental Research Institute, Seoul National University School of Dentistry, Seoul 03080, KoreaDepartment of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea; Corresponding authorCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea; Department of Neuroscience, Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Korea; Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34126, Korea; Corresponding authorSummary: The underlying mechanisms of how positive emotional valence (e.g., pleasure) causes preference of an associated context is poorly understood. Here, we show that activation of astrocytic μ-opioid receptor (MOR) drives conditioned place preference (CPP) by means of specific modulation of astrocytic MOR, an exemplar endogenous Gi protein-coupled receptor (Gi-GPCR), in the CA1 hippocampus. Long-term potentiation (LTP) induced by a subthreshold stimulation with the activation of astrocytic MOR at the Schaffer collateral pathway accounts for the memory acquisition to induce CPP. This astrocytic MOR-mediated LTP induction is dependent on astrocytic glutamate released upon activation of the astrocytic MOR and the consequent activation of the presynaptic mGluR1. The astrocytic MOR-dependent LTP and CPP were recapitulated by a chemogenetic activation of astrocyte-specifically expressed Gi-DREADD hM4Di. Our study reveals that the transduction of inhibitory Gi-signaling into augmented excitatory synaptic transmission through astrocytic glutamate is critical for the acquisition of contextual memory for CPP. : Nam et al. demonstrate that activation of hippocampal astrocytic μ-opioid receptor causes glutamate release, which increases the release probability by neuronal presynaptic mGluR1 activation and potentiates synaptic plasticity at the SC-CA1 pathway. This enhanced synaptic transmission and synaptic plasticity account for the acquisition of memory associated with CPP. Keywords: astrocyte, hippocampus, μ-opioid receptor, conditioned place preference, long-term potentiation opioidhttp://www.sciencedirect.com/science/article/pii/S2211124719308514 |
| spellingShingle | Min-Ho Nam Kyung-Seok Han Jaekwang Lee Woojin Won Wuhyun Koh Jin Young Bae Junsung Woo Jayoung Kim Elliot Kwong Tae-Yong Choi Heejung Chun Seung Eun Lee Sang-Bum Kim Ki Duk Park Se-Young Choi Yong Chul Bae C. Justin Lee Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference Cell Reports |
| title | Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference |
| title_full | Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference |
| title_fullStr | Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference |
| title_full_unstemmed | Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference |
| title_short | Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference |
| title_sort | activation of astrocytic μ opioid receptor causes conditioned place preference |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719308514 |
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