Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy
Pulp injury is one of the most common clinical diseases, and severe cases are usually associated with the functional loss of the tooth, while the current clinical treatment modality is only a cavity filling procedure without the regeneration of the dentin-pulp complex, thus leading to a devitalized...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422001429 |
| _version_ | 1797990816880263168 |
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| author | Lili Liu Xianjing Li Wenhuan Bu Nianqiang Jin Yuan Meng Yi Wang Duan Wang Xiaowei Xu Ding Zhou Hongchen Sun |
| author_facet | Lili Liu Xianjing Li Wenhuan Bu Nianqiang Jin Yuan Meng Yi Wang Duan Wang Xiaowei Xu Ding Zhou Hongchen Sun |
| author_sort | Lili Liu |
| collection | DOAJ |
| description | Pulp injury is one of the most common clinical diseases, and severe cases are usually associated with the functional loss of the tooth, while the current clinical treatment modality is only a cavity filling procedure without the regeneration of the dentin-pulp complex, thus leading to a devitalized and brittle tooth. In this study, carbon dots (CDots) with excellent biocompatibility are prepared from ascorbic acid and polyethyleneimine via a hydrothermal method. The as-prepared CDots can enhance extracellular matrix (ECM) secretion of human dental pulp stem cells (DPSCs), giving rise to increased cell adhesion on ECM and a stronger osteogenic/odontogenic differentiation capacity of DPSCs. Further, the mechanism underlying CDots-enhanced ECM secretion is revealed by the transcriptome analysis, Western blot assay and molecular dynamics simulation, identifying that the pharmacological activities of CDots are originated from a reasonable activation of the autophagy, which is mediated by regulating phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway. Based on the abundant CDots-induced ECM and thereby the reinforcement of the cell-ECM adhesion, an intact dental pulp stem cell sheet can be achieved, which in return promote in vivo the efficient regeneration of dentin-pulp complex as well as blood vessels. |
| first_indexed | 2024-04-11T08:42:20Z |
| format | Article |
| id | doaj.art-bad4b7b0acc04104b54ce799da0e90b2 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-11T08:42:20Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-bad4b7b0acc04104b54ce799da0e90b22022-12-22T04:34:11ZengElsevierMaterials Today Bio2590-00642022-12-0116100344Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagyLili Liu0Xianjing Li1Wenhuan Bu2Nianqiang Jin3Yuan Meng4Yi Wang5Duan Wang6Xiaowei Xu7Ding Zhou8Hongchen Sun9Hospital of Stomatology, Jilin University, Changchun, 130021, PR China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun, 130021, PR ChinaHospital of Stomatology, Jilin University, Changchun, 130021, PR China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun, 130021, PR ChinaSchool and Hospital of Stomatology, China Medical University, Shenyang, 110122, PR ChinaSchool and Hospital of Stomatology, China Medical University, Shenyang, 110122, PR ChinaSchool and Hospital of Stomatology, China Medical University, Shenyang, 110122, PR ChinaGraduate Program in Applied Physics, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208, USAHospital of Stomatology, Jilin University, Changchun, 130021, PR China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun, 130021, PR ChinaHospital of Stomatology, Jilin University, Changchun, 130021, PR China; Corresponding author. Hospital of Stomatology, Jilin University, Changchun, 130021, PR China.Hospital of Stomatology, Jilin University, Changchun, 130021, PR China; Corresponding author.Hospital of Stomatology, Jilin University, Changchun, 130021, PR China; Corresponding author.Pulp injury is one of the most common clinical diseases, and severe cases are usually associated with the functional loss of the tooth, while the current clinical treatment modality is only a cavity filling procedure without the regeneration of the dentin-pulp complex, thus leading to a devitalized and brittle tooth. In this study, carbon dots (CDots) with excellent biocompatibility are prepared from ascorbic acid and polyethyleneimine via a hydrothermal method. The as-prepared CDots can enhance extracellular matrix (ECM) secretion of human dental pulp stem cells (DPSCs), giving rise to increased cell adhesion on ECM and a stronger osteogenic/odontogenic differentiation capacity of DPSCs. Further, the mechanism underlying CDots-enhanced ECM secretion is revealed by the transcriptome analysis, Western blot assay and molecular dynamics simulation, identifying that the pharmacological activities of CDots are originated from a reasonable activation of the autophagy, which is mediated by regulating phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway. Based on the abundant CDots-induced ECM and thereby the reinforcement of the cell-ECM adhesion, an intact dental pulp stem cell sheet can be achieved, which in return promote in vivo the efficient regeneration of dentin-pulp complex as well as blood vessels.http://www.sciencedirect.com/science/article/pii/S2590006422001429Carbon dotsExtracellular matrixDentin-pulp complex regenerationAutophagyPI3K/Akt/mTOR signaling pathway |
| spellingShingle | Lili Liu Xianjing Li Wenhuan Bu Nianqiang Jin Yuan Meng Yi Wang Duan Wang Xiaowei Xu Ding Zhou Hongchen Sun Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy Materials Today Bio Carbon dots Extracellular matrix Dentin-pulp complex regeneration Autophagy PI3K/Akt/mTOR signaling pathway |
| title | Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy |
| title_full | Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy |
| title_fullStr | Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy |
| title_full_unstemmed | Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy |
| title_short | Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy |
| title_sort | carbon dots enhance extracellular matrix secretion for dentin pulp complex regeneration through pi3k akt mtor pathway mediated activation of autophagy |
| topic | Carbon dots Extracellular matrix Dentin-pulp complex regeneration Autophagy PI3K/Akt/mTOR signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422001429 |
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