Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations...
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| Format: | Article |
| Language: | English |
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Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo
2020-06-01
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| Series: | Revista de la Facultad de Ciencias Agrarias |
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| Online Access: | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/3075 |
| _version_ | 1797756038789726208 |
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| author | Daniel López-Lima Gloria Carrión Petra Sánchez-Nava Damaris Desgarennes Luc Villain |
| author_facet | Daniel López-Lima Gloria Carrión Petra Sánchez-Nava Damaris Desgarennes Luc Villain |
| author_sort | Daniel López-Lima |
| collection | DOAJ |
| description |
The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations by corky-root disease. Previous studies have revealed the presence of fungi in coffee corky-root tissues. However, these fungi have not been yet identified. This work aimed to identify at species level the fungi associated to the coffee corky-root symptoms and determine their pathogenicity on coffee plants. Coffee roots with corky-root symptoms were collected in eight sites distributed through the major coffee growing region of Veracruz. Observations of inside cortical root tissues under scanning electron microscope revealed abundant mycelium and conidia in
corky-root samples in contrast with absence of any fungi development in healthy roots. Forty-nine fungi strains from internal corky-root tissue were isolated and identified at species level by ITS sequences. Fusarium oxysporum was the most frequent species and the only present in all of the corky-root samples. These strains were selected for the pathogenicity test. All F. oxysporum strains colonized the vascular system of coffee plants although none caused wilting symptoms.
Highlights
Fusarium oxysporum is a major fungus associated with coffee corky-root disease.
None of the oxysporum strains inoculated into the roots of coffee plants caused vascular withering symptoms
In the tissues damaged by coffee corky-root disease interact nematodes, fungi and bacteria.
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| first_indexed | 2024-03-12T17:55:47Z |
| format | Article |
| id | doaj.art-bad66c58026f42f2bbd0ee9d6fa1e7b6 |
| institution | Directory Open Access Journal |
| issn | 0370-4661 1853-8665 |
| language | English |
| last_indexed | 2024-03-12T17:55:47Z |
| publishDate | 2020-06-01 |
| publisher | Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo |
| record_format | Article |
| series | Revista de la Facultad de Ciencias Agrarias |
| spelling | doaj.art-bad66c58026f42f2bbd0ee9d6fa1e7b62023-08-02T17:57:03ZengFacultad de Ciencias Agrarias. Universidad Nacional de CuyoRevista de la Facultad de Ciencias Agrarias0370-46611853-86652020-06-01521Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in MexicoDaniel López-Lima0Gloria Carrión1Petra Sánchez-Nava2Damaris Desgarennes3Luc Villain4Instituto de Ecología, A.C.-Cluster Biomimic. Carretera antigua a Coatepec 351. Xalapa 91070. Veracruz. México. Instituto de Ecología, A.C.-Cluster Biomimic. Carretera antigua a Coatepec 351. Xalapa 91070. Veracruz. México.Universidad Autónoma del Estado de México. Instituto Literario 100. Toluca 50000. Estado de México. Mexico. 1 Instituto de Ecología, A.C.-Cluster Biomimic. Carretera antigua a Coatepec 351. Xalapa 91070. Veracruz. México.Cirad. UMR RPB, F- 34394 Montpellier. France. The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations by corky-root disease. Previous studies have revealed the presence of fungi in coffee corky-root tissues. However, these fungi have not been yet identified. This work aimed to identify at species level the fungi associated to the coffee corky-root symptoms and determine their pathogenicity on coffee plants. Coffee roots with corky-root symptoms were collected in eight sites distributed through the major coffee growing region of Veracruz. Observations of inside cortical root tissues under scanning electron microscope revealed abundant mycelium and conidia in corky-root samples in contrast with absence of any fungi development in healthy roots. Forty-nine fungi strains from internal corky-root tissue were isolated and identified at species level by ITS sequences. Fusarium oxysporum was the most frequent species and the only present in all of the corky-root samples. These strains were selected for the pathogenicity test. All F. oxysporum strains colonized the vascular system of coffee plants although none caused wilting symptoms. Highlights Fusarium oxysporum is a major fungus associated with coffee corky-root disease. None of the oxysporum strains inoculated into the roots of coffee plants caused vascular withering symptoms In the tissues damaged by coffee corky-root disease interact nematodes, fungi and bacteria. https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/3075Coffea arabicaroot-knot nematodesfilamentous fungi |
| spellingShingle | Daniel López-Lima Gloria Carrión Petra Sánchez-Nava Damaris Desgarennes Luc Villain Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico Revista de la Facultad de Ciencias Agrarias Coffea arabica root-knot nematodes filamentous fungi |
| title | Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico |
| title_full | Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico |
| title_fullStr | Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico |
| title_full_unstemmed | Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico |
| title_short | Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico |
| title_sort | fungal diversity and fusarium oxysporum pathogenicity associated with coffee corky root disease in mexico |
| topic | Coffea arabica root-knot nematodes filamentous fungi |
| url | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/3075 |
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