Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA
Context: The incidence of solar ultraviolet radiation (UV) on Earth has increased due to diminish of the ozone layer. This enviromental agent is highly genotoxic causing numerous damage in DNA molecule. Nowadays there is a growing interest in the search of compounds capable to minimize these effects...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2017-08-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol5/jppres17.211_5.4.262.pdf |
| _version_ | 1818792148864073728 |
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| author | Marioly Vernhes Tamayo André Schuch Passaglia Fabiana Fuentes-León Carlos F. Martins Menck Ángel Sánchez-Lamar |
| author_facet | Marioly Vernhes Tamayo André Schuch Passaglia Fabiana Fuentes-León Carlos F. Martins Menck Ángel Sánchez-Lamar |
| author_sort | Marioly Vernhes Tamayo |
| collection | DOAJ |
| description | Context: The incidence of solar ultraviolet radiation (UV) on Earth has increased due to diminish of the ozone layer. This enviromental agent is highly genotoxic causing numerous damage in DNA molecule. Nowadays there is a growing interest in the search of compounds capable to minimize these effects. In particular, phytocompounds have been tested as excelent candidates for their antigenotoxic properties.
Aims: To evaluate the protective effect of the aqueous extract of Pinus caribaea (EPC) against the damage induced by the UVB and UVC radiation.
Methods: The cell-free plasmid DNA assay was employed. The forms of plasmid were separated electrophoretically in agarose gel. For genotoxic and photoprotective evaluation of P. caribaea, different concentrations of the extract (0.1 – 2.0 mg/mL) and exposure times were evaluated. The CPD lesions were detected enzymatically. Additionally, the transmittance of the aqueous extract against 254 nm and 312 nm was measured.
Results: None of the concentrations were genotoxic in 30 min of treatment, for superior times a clastogenic effect was observed. The EPC despite inhibiting the activity of the enzyme T4 endo V, impedes photolesions formation in DNA at concentrations ≥ 0.1 mg/mL.
Conclusions: The EPC has photoprotective properties, this effect could be related with its antioxidants and absorptives capacities. |
| first_indexed | 2024-12-18T15:22:39Z |
| format | Article |
| id | doaj.art-bada18b3f2f94fca83905f7715eaf8d7 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-18T15:22:39Z |
| publishDate | 2017-08-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-bada18b3f2f94fca83905f7715eaf8d72022-12-21T21:03:21ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502017-08-0154262269Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNAMarioly Vernhes Tamayo0André Schuch Passaglia1Fabiana Fuentes-León2Carlos F. Martins Menck3Ángel Sánchez-Lamar4Departamento de Radiobiología, Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN). Apartado Postal 6122, Calle 30, #502, e/5ta y 7ma, Miramar, Playa, La Habana, Cuba.Departamento de Microbiología, Instituto de Ciencias Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374 Ed. Biomédicas 2, São Paulo, SP, Brasil.Departamento de Biología Vegetal, Facultad de Biología, Universidad de La Habana, Apartado Postal 10 400, Calle 25, # 455, e/ I y J, Vedado, Plaza de la Revolución, La Habana, Cuba.Departamento de Microbiología, Instituto de Ciencias Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374 Ed. Biomédicas 2, São Paulo, SP, Brasil.Departamento de Biología Vegetal, Facultad de Biología, Universidad de La Habana, Apartado Postal 10 400, Calle 25, # 455, e/ I y J, Vedado, Plaza de la Revolución, La Habana, Cuba.Context: The incidence of solar ultraviolet radiation (UV) on Earth has increased due to diminish of the ozone layer. This enviromental agent is highly genotoxic causing numerous damage in DNA molecule. Nowadays there is a growing interest in the search of compounds capable to minimize these effects. In particular, phytocompounds have been tested as excelent candidates for their antigenotoxic properties. Aims: To evaluate the protective effect of the aqueous extract of Pinus caribaea (EPC) against the damage induced by the UVB and UVC radiation. Methods: The cell-free plasmid DNA assay was employed. The forms of plasmid were separated electrophoretically in agarose gel. For genotoxic and photoprotective evaluation of P. caribaea, different concentrations of the extract (0.1 – 2.0 mg/mL) and exposure times were evaluated. The CPD lesions were detected enzymatically. Additionally, the transmittance of the aqueous extract against 254 nm and 312 nm was measured. Results: None of the concentrations were genotoxic in 30 min of treatment, for superior times a clastogenic effect was observed. The EPC despite inhibiting the activity of the enzyme T4 endo V, impedes photolesions formation in DNA at concentrations ≥ 0.1 mg/mL. Conclusions: The EPC has photoprotective properties, this effect could be related with its antioxidants and absorptives capacities.http://jppres.com/jppres/pdf/vol5/jppres17.211_5.4.262.pdfphotolesionsphotoprotectionplant extractUV |
| spellingShingle | Marioly Vernhes Tamayo André Schuch Passaglia Fabiana Fuentes-León Carlos F. Martins Menck Ángel Sánchez-Lamar Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA Journal of Pharmacy & Pharmacognosy Research photolesions photoprotection plant extract UV |
| title | Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA |
| title_full | Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA |
| title_fullStr | Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA |
| title_full_unstemmed | Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA |
| title_short | Aqueous extract of Pinus caribaea inhibits the damage induced by ultraviolet radiations, in plasmid DNA |
| title_sort | aqueous extract of pinus caribaea inhibits the damage induced by ultraviolet radiations in plasmid dna |
| topic | photolesions photoprotection plant extract UV |
| url | http://jppres.com/jppres/pdf/vol5/jppres17.211_5.4.262.pdf |
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