Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation

Repeated batch cultivation is known as most attractive method in improving hydrogen productivity, due to the facts that this approach could minimize the reuse of the cell and the inoculum preparation. In addition, with the combination of attach growth system during the fermentation processes to prod...

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Main Authors: Idris Norfatiha, Lutpi Nabilah Aminah, Che Ridzuan Che Mohd Ruhaizul, Shian Wong Yee, Tengku Izhar Tengku Nuraiti
Format: Article
Language:English
Published: EDP Sciences 2018-01-01
Series:E3S Web of Conferences
Online Access:https://doi.org/10.1051/e3sconf/20183402044
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author Idris Norfatiha
Lutpi Nabilah Aminah
Che Ridzuan Che Mohd Ruhaizul
Shian Wong Yee
Tengku Izhar Tengku Nuraiti
author_facet Idris Norfatiha
Lutpi Nabilah Aminah
Che Ridzuan Che Mohd Ruhaizul
Shian Wong Yee
Tengku Izhar Tengku Nuraiti
author_sort Idris Norfatiha
collection DOAJ
description Repeated batch cultivation is known as most attractive method in improving hydrogen productivity, due to the facts that this approach could minimize the reuse of the cell and the inoculum preparation. In addition, with the combination of attach growth system during the fermentation processes to produce biohydrogen, the density of cells will be increased and the cell washout could be avoided. Therefore, this study aimed to examine the effectiveness of repeated batch cultivation for enrichment of anaerobic mixed culture onto granular activated carbon (GAC) and investigate the effect of molasses concentration during immobilization of mixed culture onto the GAC. The molasses concentration using 50 %, 40 %, 30 %, 20 % and 10 % of diluted molasses were used as feedstock in the fermentation process. The maximum hydrogen production of 60 ml was obtained at 30 % of molasses concentration with 831 ppm of hydrogen concentration. Thus, the kinetic parameter obtained from the batch profiling based on modified Gompertz equation are, Hm= 58 ml for the maximum hydrogen production and Rm= 2.02 ml/h representing the hydrogen production rate.
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spelling doaj.art-badc74a7095149c8bb23bbe9e4e2104e2022-12-21T22:40:26ZengEDP SciencesE3S Web of Conferences2267-12422018-01-01340204410.1051/e3sconf/20183402044e3sconf_cenviron2018_02044Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch CultivationIdris NorfatihaLutpi Nabilah AminahChe Ridzuan Che Mohd RuhaizulShian Wong YeeTengku Izhar Tengku NuraitiRepeated batch cultivation is known as most attractive method in improving hydrogen productivity, due to the facts that this approach could minimize the reuse of the cell and the inoculum preparation. In addition, with the combination of attach growth system during the fermentation processes to produce biohydrogen, the density of cells will be increased and the cell washout could be avoided. Therefore, this study aimed to examine the effectiveness of repeated batch cultivation for enrichment of anaerobic mixed culture onto granular activated carbon (GAC) and investigate the effect of molasses concentration during immobilization of mixed culture onto the GAC. The molasses concentration using 50 %, 40 %, 30 %, 20 % and 10 % of diluted molasses were used as feedstock in the fermentation process. The maximum hydrogen production of 60 ml was obtained at 30 % of molasses concentration with 831 ppm of hydrogen concentration. Thus, the kinetic parameter obtained from the batch profiling based on modified Gompertz equation are, Hm= 58 ml for the maximum hydrogen production and Rm= 2.02 ml/h representing the hydrogen production rate.https://doi.org/10.1051/e3sconf/20183402044
spellingShingle Idris Norfatiha
Lutpi Nabilah Aminah
Che Ridzuan Che Mohd Ruhaizul
Shian Wong Yee
Tengku Izhar Tengku Nuraiti
Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation
E3S Web of Conferences
title Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation
title_full Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation
title_fullStr Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation
title_full_unstemmed Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation
title_short Study on Molasses Concentration from Sugarcanne Bagasse for Biohydrogen Production using Enriched Granular Activated Carbon (GAC) Immobilised Cells by Repeated Batch Cultivation
title_sort study on molasses concentration from sugarcanne bagasse for biohydrogen production using enriched granular activated carbon gac immobilised cells by repeated batch cultivation
url https://doi.org/10.1051/e3sconf/20183402044
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