Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In thi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.773092/full |
| _version_ | 1797745140969766912 |
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| author | Laura Toral Miguel Rodríguez Miguel Rodríguez Fernando Martínez-Checa Fernando Martínez-Checa Alfredo Montaño Amparo Cortés-Delgado Agnieszka Smolinska Inmaculada Llamas Inmaculada Llamas Inmaculada Sampedro Inmaculada Sampedro |
| author_facet | Laura Toral Miguel Rodríguez Miguel Rodríguez Fernando Martínez-Checa Fernando Martínez-Checa Alfredo Montaño Amparo Cortés-Delgado Agnieszka Smolinska Inmaculada Llamas Inmaculada Llamas Inmaculada Sampedro Inmaculada Sampedro |
| author_sort | Laura Toral |
| collection | DOAJ |
| description | Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas, Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth inhibition assays and in vivo experiments using cherry fruits showed that the best producers of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa, which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death. |
| first_indexed | 2024-03-12T15:19:14Z |
| format | Article |
| id | doaj.art-badfee66dc9746a19dd6425e1ba946a9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T15:19:14Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-badfee66dc9746a19dd6425e1ba946a92023-08-11T06:10:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.773092773092Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal PhytopathogensLaura Toral0Miguel Rodríguez1Miguel Rodríguez2Fernando Martínez-Checa3Fernando Martínez-Checa4Alfredo Montaño5Amparo Cortés-Delgado6Agnieszka Smolinska7Inmaculada Llamas8Inmaculada Llamas9Inmaculada Sampedro10Inmaculada Sampedro11Xtrem Biotech S.L., European Business Innovation Center, Avenida de la Innovación, Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, Campus de Cartuja s/n, Granada, SpainBiomedical Research Center (CIBM), Avenida del Conocimiento s/n, Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, Campus de Cartuja s/n, Granada, SpainBiomedical Research Center (CIBM), Avenida del Conocimiento s/n, Granada, SpainDepartment of Food Biotechnology, Instituto de la Grasa, Sevilla, SpainDepartment of Food Biotechnology, Instituto de la Grasa, Sevilla, SpainDepartment of Pharmacology and Toxicology, Maastricht University, Maastricht, NetherlandsDepartment of Microbiology, Faculty of Pharmacy, Campus de Cartuja s/n, Granada, SpainBiomedical Research Center (CIBM), Avenida del Conocimiento s/n, Granada, SpainDepartment of Microbiology, Faculty of Pharmacy, Campus de Cartuja s/n, Granada, SpainBiomedical Research Center (CIBM), Avenida del Conocimiento s/n, Granada, SpainPhytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas, Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth inhibition assays and in vivo experiments using cherry fruits showed that the best producers of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa, which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death.https://www.frontiersin.org/articles/10.3389/fmicb.2021.773092/fullvolatile compoundsantifungal activitybiocontrolfungal phytopathogenspostharvest diseases |
| spellingShingle | Laura Toral Miguel Rodríguez Miguel Rodríguez Fernando Martínez-Checa Fernando Martínez-Checa Alfredo Montaño Amparo Cortés-Delgado Agnieszka Smolinska Inmaculada Llamas Inmaculada Llamas Inmaculada Sampedro Inmaculada Sampedro Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens Frontiers in Microbiology volatile compounds antifungal activity biocontrol fungal phytopathogens postharvest diseases |
| title | Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens |
| title_full | Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens |
| title_fullStr | Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens |
| title_full_unstemmed | Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens |
| title_short | Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens |
| title_sort | identification of volatile organic compounds in extremophilic bacteria and their effective use in biocontrol of postharvest fungal phytopathogens |
| topic | volatile compounds antifungal activity biocontrol fungal phytopathogens postharvest diseases |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.773092/full |
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