Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples
(1) Pigs are physiologically very relevant as animal models of human physiology. Radiotracer methods for porcine GFR (glomerular filtration rate) determination exist but require full-curve blood sampling or the application of correction formulas, which vary among studies. (2) We used porcine GFR dat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Biologics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8449/3/4/15 |
| _version_ | 1797381986844672000 |
|---|---|
| author | Lars Jødal Juan Ignacio Brignone Pui-Ki Chan Ladefoged Lars Lund Trine Borup Andersen |
| author_facet | Lars Jødal Juan Ignacio Brignone Pui-Ki Chan Ladefoged Lars Lund Trine Borup Andersen |
| author_sort | Lars Jødal |
| collection | DOAJ |
| description | (1) Pigs are physiologically very relevant as animal models of human physiology. Radiotracer methods for porcine GFR (glomerular filtration rate) determination exist but require full-curve blood sampling or the application of correction formulas, which vary among studies. (2) We used porcine GFR data (40 datapoints from 20 juvenile pigs) for which the GFR was measured as the plasma clearance of [<sup>99m</sup>Tc]Tc-DTPA. The reference clearance (<i>Cl</i>, GFR; range 41–85 mL/min) was measured from the full curve under the data. For simpler determination, an approximate clearance, <i>Cl</i><sub>1</sub>, was based on the last five blood samples (acquired 120–240 min post injection). (3) The following formula for the GFR was developed: <i>Cl</i> = 1.27 · (<i>Cl</i><sub>1</sub>)<sup>0.92</sup>. The spread (SD) was within 4% of the reference GFR. A comparison with the literature data showed that our correction formula was robust in pigs of various breeds, sizes up to approximately 200 kg, and GFRs up to approximately 400 mL/min, with a spread of up to 8%. The formula was also applicable for iohexol as the tracer. (4) A formula was developed that allows porcine GFR to be measured using only 4–5 late blood samples. This new formula can be applied across a wide range of swine breeds, animal sizes, and GFR ranges, allowing for robust determination of the GFR in pigs without full-curve blood sampling and without urine collection. |
| first_indexed | 2024-03-08T20:59:59Z |
| format | Article |
| id | doaj.art-bae1b7d003a84d76bcdf16674c3f9cd2 |
| institution | Directory Open Access Journal |
| issn | 2673-8449 |
| language | English |
| last_indexed | 2024-03-08T20:59:59Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biologics |
| spelling | doaj.art-bae1b7d003a84d76bcdf16674c3f9cd22023-12-22T13:54:17ZengMDPI AGBiologics2673-84492023-09-013429630710.3390/biologics3040015Robust Porcine GFR Measurement with Radiotracers and Only Late Blood SamplesLars Jødal0Juan Ignacio Brignone1Pui-Ki Chan Ladefoged2Lars Lund3Trine Borup Andersen4Department of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Urology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Urology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Nuclear Medicine, Aalborg University Hospital, 9000 Aalborg, Denmark(1) Pigs are physiologically very relevant as animal models of human physiology. Radiotracer methods for porcine GFR (glomerular filtration rate) determination exist but require full-curve blood sampling or the application of correction formulas, which vary among studies. (2) We used porcine GFR data (40 datapoints from 20 juvenile pigs) for which the GFR was measured as the plasma clearance of [<sup>99m</sup>Tc]Tc-DTPA. The reference clearance (<i>Cl</i>, GFR; range 41–85 mL/min) was measured from the full curve under the data. For simpler determination, an approximate clearance, <i>Cl</i><sub>1</sub>, was based on the last five blood samples (acquired 120–240 min post injection). (3) The following formula for the GFR was developed: <i>Cl</i> = 1.27 · (<i>Cl</i><sub>1</sub>)<sup>0.92</sup>. The spread (SD) was within 4% of the reference GFR. A comparison with the literature data showed that our correction formula was robust in pigs of various breeds, sizes up to approximately 200 kg, and GFRs up to approximately 400 mL/min, with a spread of up to 8%. The formula was also applicable for iohexol as the tracer. (4) A formula was developed that allows porcine GFR to be measured using only 4–5 late blood samples. This new formula can be applied across a wide range of swine breeds, animal sizes, and GFR ranges, allowing for robust determination of the GFR in pigs without full-curve blood sampling and without urine collection.https://www.mdpi.com/2673-8449/3/4/15glomerular filtration raterenal functionpreclinicalpigsswinetracer methods |
| spellingShingle | Lars Jødal Juan Ignacio Brignone Pui-Ki Chan Ladefoged Lars Lund Trine Borup Andersen Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples Biologics glomerular filtration rate renal function preclinical pigs swine tracer methods |
| title | Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples |
| title_full | Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples |
| title_fullStr | Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples |
| title_full_unstemmed | Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples |
| title_short | Robust Porcine GFR Measurement with Radiotracers and Only Late Blood Samples |
| title_sort | robust porcine gfr measurement with radiotracers and only late blood samples |
| topic | glomerular filtration rate renal function preclinical pigs swine tracer methods |
| url | https://www.mdpi.com/2673-8449/3/4/15 |
| work_keys_str_mv | AT larsjødal robustporcinegfrmeasurementwithradiotracersandonlylatebloodsamples AT juanignaciobrignone robustporcinegfrmeasurementwithradiotracersandonlylatebloodsamples AT puikichanladefoged robustporcinegfrmeasurementwithradiotracersandonlylatebloodsamples AT larslund robustporcinegfrmeasurementwithradiotracersandonlylatebloodsamples AT trineborupandersen robustporcinegfrmeasurementwithradiotracersandonlylatebloodsamples |