Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/6/699 |
| _version_ | 1797530549440479232 |
|---|---|
| author | Hui Han Mohan Bai Yanting Chen Yali Gong Ming Wu Hefa Yang Qing Chen Ting Xu Yuquan Wei Guochun Ding Ji Li |
| author_facet | Hui Han Mohan Bai Yanting Chen Yali Gong Ming Wu Hefa Yang Qing Chen Ting Xu Yuquan Wei Guochun Ding Ji Li |
| author_sort | Hui Han |
| collection | DOAJ |
| description | Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (<i>sul1</i> and <i>sul2</i>) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg<sup>−1</sup>) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both <i>sul1</i> and <i>sul2</i> were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for <i>sul2</i> in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with <i>sul1</i> and <i>sul2</i> were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) <i>sul1</i> and <i>sul2</i> genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine. |
| first_indexed | 2024-03-10T10:30:35Z |
| format | Article |
| id | doaj.art-bae5c455e0a247238ac8027db996220a |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:30:35Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-bae5c455e0a247238ac8027db996220a2023-11-21T23:41:35ZengMDPI AGAntibiotics2079-63822021-06-0110669910.3390/antibiotics10060699Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by CompostHui Han0Mohan Bai1Yanting Chen2Yali Gong3Ming Wu4Hefa Yang5Qing Chen6Ting Xu7Yuquan Wei8Guochun Ding9Ji Li10College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaQuzhou Experimental Station of China Agricultural University, Quzhou 057250, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaCollege of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, ChinaAlthough composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (<i>sul1</i> and <i>sul2</i>) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg<sup>−1</sup>) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both <i>sul1</i> and <i>sul2</i> were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for <i>sul2</i> in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with <i>sul1</i> and <i>sul2</i> were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) <i>sul1</i> and <i>sul2</i> genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine.https://www.mdpi.com/2079-6382/10/6/699compostsulfadiazine resistancehigh throughput sequencing<i>sul1</i><i>sul2</i> |
| spellingShingle | Hui Han Mohan Bai Yanting Chen Yali Gong Ming Wu Hefa Yang Qing Chen Ting Xu Yuquan Wei Guochun Ding Ji Li Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost Antibiotics compost sulfadiazine resistance high throughput sequencing <i>sul1</i> <i>sul2</i> |
| title | Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost |
| title_full | Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost |
| title_fullStr | Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost |
| title_full_unstemmed | Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost |
| title_short | Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost |
| title_sort | dynamics of diversity and abundance of sulfonamide resistant bacteria in a silt loam soil fertilized by compost |
| topic | compost sulfadiazine resistance high throughput sequencing <i>sul1</i> <i>sul2</i> |
| url | https://www.mdpi.com/2079-6382/10/6/699 |
| work_keys_str_mv | AT huihan dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT mohanbai dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT yantingchen dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT yaligong dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT mingwu dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT hefayang dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT qingchen dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT tingxu dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT yuquanwei dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT guochunding dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost AT jili dynamicsofdiversityandabundanceofsulfonamideresistantbacteriainasiltloamsoilfertilizedbycompost |