EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC
Synthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation of isolated cells with human serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A consti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/64 |
| _version_ | 1797198181521424384 |
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| author | S. B. Cheknev M. V. Mezentseva I. M. Shapoval A. N. Narovlyansky |
| author_facet | S. B. Cheknev M. V. Mezentseva I. M. Shapoval A. N. Narovlyansky |
| author_sort | S. B. Cheknev |
| collection | DOAJ |
| description | Synthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation of isolated cells with human serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A constitutive presence of interferon-α (IFNα) and interleukin-18 (IL-18) mRNA was shown in MNC of all of the subjects observed, whereas IFNγ, IL-4, or IL-8 mRNA synthesis was not detectable. Expression of IL-1β, IL-2, IL-6 и IL-10 was revealed in only a half of cases. It has been shown that pure γ-globulins did induce IFNγ gene expression and enhance transcriptional activity of IL-1β and IL-2 genes. Zinc cations did also promote transcription of IL-1β gene, along with reduced synthesis of IL-2 and IFNγ mRNA, compared to the γ-globulin. In presence of γ-globulin-zinc complexes, IL-1β mRNA was synthesized at the same level, as following induction with control γ-globulin, whereas IL-2 gene expression was reduced, and IFNγ mRNA synthesis was abolished. The results are discussed in terms of immune response polarization in presence of γ-globulins and their metal complexes with zinc. |
| first_indexed | 2024-03-08T05:51:54Z |
| format | Article |
| id | doaj.art-bae762c3af454c1b9b9ba2347ae17386 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:55:47Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-bae762c3af454c1b9b9ba2347ae173862024-04-22T13:07:32ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0112317117610.15789/1563-0625-2010-3-171-17663EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINCS. B. Cheknev0M. V. Mezentseva1I. M. Shapoval2A. N. Narovlyansky3Лаборатория межклеточных взаимодействий, лаборатория микробиологии латентных инфекций, лаборатория цитокинов НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН, МоскваЛаборатория межклеточных взаимодействий, лаборатория микробиологии латентных инфекций, лаборатория цитокинов НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН, МоскваЛаборатория межклеточных взаимодействий, лаборатория микробиологии латентных инфекций, лаборатория цитокинов НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН, МоскваЛаборатория межклеточных взаимодействий, лаборатория микробиологии латентных инфекций, лаборатория цитокинов НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи РАМН, МоскваSynthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation of isolated cells with human serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A constitutive presence of interferon-α (IFNα) and interleukin-18 (IL-18) mRNA was shown in MNC of all of the subjects observed, whereas IFNγ, IL-4, or IL-8 mRNA synthesis was not detectable. Expression of IL-1β, IL-2, IL-6 и IL-10 was revealed in only a half of cases. It has been shown that pure γ-globulins did induce IFNγ gene expression and enhance transcriptional activity of IL-1β and IL-2 genes. Zinc cations did also promote transcription of IL-1β gene, along with reduced synthesis of IL-2 and IFNγ mRNA, compared to the γ-globulin. In presence of γ-globulin-zinc complexes, IL-1β mRNA was synthesized at the same level, as following induction with control γ-globulin, whereas IL-2 gene expression was reduced, and IFNγ mRNA synthesis was abolished. The results are discussed in terms of immune response polarization in presence of γ-globulins and their metal complexes with zinc.https://www.mimmun.ru/mimmun/article/view/64γ-globulinmetal complexeszincgenescytokinesexpression |
| spellingShingle | S. B. Cheknev M. V. Mezentseva I. M. Shapoval A. N. Narovlyansky EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC Медицинская иммунология γ-globulin metal complexes zinc genes cytokines expression |
| title | EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC |
| title_full | EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC |
| title_fullStr | EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC |
| title_full_unstemmed | EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC |
| title_short | EXPRESSION OF THE CYTOKINE GENES INDUCED BY HUMAN SERUM γ-GLOBULIN AND BY ITS METAL COMPLEXES WITH ZINC |
| title_sort | expression of the cytokine genes induced by human serum γ globulin and by its metal complexes with zinc |
| topic | γ-globulin metal complexes zinc genes cytokines expression |
| url | https://www.mimmun.ru/mimmun/article/view/64 |
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