Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars
Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymo...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2023-04-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/13629 |
| _version_ | 1797814298332889088 |
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| author | Abbas SAIDI Melika HAJKAZEMIAN |
| author_facet | Abbas SAIDI Melika HAJKAZEMIAN |
| author_sort | Abbas SAIDI |
| collection | DOAJ |
| description |
Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity.
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| first_indexed | 2024-03-13T08:05:34Z |
| format | Article |
| id | doaj.art-bae7dd6f433b4e94a4e027acea900676 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-13T08:05:34Z |
| publishDate | 2023-04-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-bae7dd6f433b4e94a4e027acea9006762023-06-01T07:17:23ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412023-04-01119110.14720/aas.2023.119.1.2425Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivarsAbbas SAIDI0Melika HAJKAZEMIAN1Faculty of Life Sciences and Biotechnology, Department of Plant Sciences and Biotechnology, Shahid Beheshti University, TehranFaculty of Life Sciences and Biotechnology, Department of Plant Sciences and Biotechnology, Shahid Beheshti University, Tehran Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity. https://journals.uni-lj.si/aas/article/view/13629GerberaDAMDgenetic diversityISSR |
| spellingShingle | Abbas SAIDI Melika HAJKAZEMIAN Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars Acta Agriculturae Slovenica Gerbera DAMD genetic diversity ISSR |
| title | Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars |
| title_full | Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars |
| title_fullStr | Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars |
| title_full_unstemmed | Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars |
| title_short | Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars |
| title_sort | comparative assessment of issr damd and rapd markers for evaluation of genetic diversity of gerbera gerbera jamesonii bolus ex hooker f cultivars |
| topic | Gerbera DAMD genetic diversity ISSR |
| url | https://journals.uni-lj.si/aas/article/view/13629 |
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