Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping
This study aimed to develop polycaprolactone (PCL) electrospun membranes coated with mineral trioxide aggregate/hydroxyapatite (MTA/HA) as a potential material for dental pulp capping. Initially, the PCL membrane was prepared by an electrospinning process, which was further surface coated with MTA (...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/22/4862 |
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| author | Soumya Sheela Fatma Mousa AlGhalban Khalil Abdelrazek Khalil Tahar Laoui Vellore Kannan Gopinath |
| author_facet | Soumya Sheela Fatma Mousa AlGhalban Khalil Abdelrazek Khalil Tahar Laoui Vellore Kannan Gopinath |
| author_sort | Soumya Sheela |
| collection | DOAJ |
| description | This study aimed to develop polycaprolactone (PCL) electrospun membranes coated with mineral trioxide aggregate/hydroxyapatite (MTA/HA) as a potential material for dental pulp capping. Initially, the PCL membrane was prepared by an electrospinning process, which was further surface coated with MTA (labeled as PCLMTA) and HA (labeled as PCLHA). The physico-chemical characterization of the fabricated membranes was carried out using field emission scanning electron microscopy (FE-SEM)/Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Raman spectroscopy, and contact angle analysis. The biocompatibility of the human dental pulp stem cells (hDPSCs) on the fabricated membranes was checked by XTT assay, and the hDPSCs adhesion and spreading were assessed by FE-SEM and confocal microscopy. The wound healing ability of hDPSCs in response to different electrospun membrane extracts was examined by scratch assay. The surface morphology analysis of the membranes by FE-SEM demonstrated a uniform nanofibrous texture with an average fiber diameter of 594 ± 124 nm for PCL, 517 ± 159 nm for PCLHA, and 490 ± 162 nm for PCLMTA. The elemental analysis of the PCLHA membrane indicated the presence of calcium and phosphorous elements related to HA, whereas the PCLMTA membrane showed the presence of calcium and silicate, related to MTA. The presence of MTA and HA in the PCL membranes was also confirmed by Raman spectroscopy. The water contact analysis demonstrated the hydrophobic nature of the membranes. The results indicated that PCL, PCLHA, and PCLMTA membranes were biocompatible, while PCLMTA exhibited better cell adhesion, spreading, and migration. |
| first_indexed | 2024-03-09T18:03:17Z |
| format | Article |
| id | doaj.art-bae7e6f8f637431bb59930bb5c67611c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T18:03:17Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-bae7e6f8f637431bb59930bb5c67611c2023-11-24T09:42:14ZengMDPI AGPolymers2073-43602022-11-011422486210.3390/polym14224862Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp CappingSoumya Sheela0Fatma Mousa AlGhalban1Khalil Abdelrazek Khalil2Tahar Laoui3Vellore Kannan Gopinath4Sharjah Institute for Medical Research, University of Sharjah, Sharjah 27272, United Arab EmiratesSharjah Institute for Medical Research, University of Sharjah, Sharjah 27272, United Arab EmiratesDepartment of Mechanical & Nuclear Engineering, College of Engineering, University of Sharjah, Sharjah 27272, United Arab EmiratesDepartment of Mechanical & Nuclear Engineering, College of Engineering, University of Sharjah, Sharjah 27272, United Arab EmiratesSharjah Institute for Medical Research, University of Sharjah, Sharjah 27272, United Arab EmiratesThis study aimed to develop polycaprolactone (PCL) electrospun membranes coated with mineral trioxide aggregate/hydroxyapatite (MTA/HA) as a potential material for dental pulp capping. Initially, the PCL membrane was prepared by an electrospinning process, which was further surface coated with MTA (labeled as PCLMTA) and HA (labeled as PCLHA). The physico-chemical characterization of the fabricated membranes was carried out using field emission scanning electron microscopy (FE-SEM)/Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Raman spectroscopy, and contact angle analysis. The biocompatibility of the human dental pulp stem cells (hDPSCs) on the fabricated membranes was checked by XTT assay, and the hDPSCs adhesion and spreading were assessed by FE-SEM and confocal microscopy. The wound healing ability of hDPSCs in response to different electrospun membrane extracts was examined by scratch assay. The surface morphology analysis of the membranes by FE-SEM demonstrated a uniform nanofibrous texture with an average fiber diameter of 594 ± 124 nm for PCL, 517 ± 159 nm for PCLHA, and 490 ± 162 nm for PCLMTA. The elemental analysis of the PCLHA membrane indicated the presence of calcium and phosphorous elements related to HA, whereas the PCLMTA membrane showed the presence of calcium and silicate, related to MTA. The presence of MTA and HA in the PCL membranes was also confirmed by Raman spectroscopy. The water contact analysis demonstrated the hydrophobic nature of the membranes. The results indicated that PCL, PCLHA, and PCLMTA membranes were biocompatible, while PCLMTA exhibited better cell adhesion, spreading, and migration.https://www.mdpi.com/2073-4360/14/22/4862polycaprolactonehuman dental pulp stem cellspulp cappinghydroxyapatitemineral trioxide aggregateelectrospinning |
| spellingShingle | Soumya Sheela Fatma Mousa AlGhalban Khalil Abdelrazek Khalil Tahar Laoui Vellore Kannan Gopinath Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping Polymers polycaprolactone human dental pulp stem cells pulp capping hydroxyapatite mineral trioxide aggregate electrospinning |
| title | Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping |
| title_full | Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping |
| title_fullStr | Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping |
| title_full_unstemmed | Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping |
| title_short | Synthesis and Biocompatibility Evaluation of PCL Electrospun Membranes Coated with MTA/HA for Potential Application in Dental Pulp Capping |
| title_sort | synthesis and biocompatibility evaluation of pcl electrospun membranes coated with mta ha for potential application in dental pulp capping |
| topic | polycaprolactone human dental pulp stem cells pulp capping hydroxyapatite mineral trioxide aggregate electrospinning |
| url | https://www.mdpi.com/2073-4360/14/22/4862 |
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