Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells
Abstract Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhib...
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| Format: | Article |
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The Korean Society of Fisheries and Aquatic Science
2019-03-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41240-019-0122-7 |
| _version_ | 1831685296217915392 |
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| author | Wi-Gyeong Gwon Sang-Gil Lee Jae-Il Kim Young-Mog Kim Seon-Bong Kim Hyeung-Rak Kim |
| author_facet | Wi-Gyeong Gwon Sang-Gil Lee Jae-Il Kim Young-Mog Kim Seon-Bong Kim Hyeung-Rak Kim |
| author_sort | Wi-Gyeong Gwon |
| collection | DOAJ |
| description | Abstract Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhibition of tumor necrosis factor (TNF)-α-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). We found that HSS suppressed the production of cell adhesion molecules such as intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 in TNF-α-induced HUVECs. Moreover, TNF-α-induced production of monocyte chemoattractant protein 1 and keratinocyte chemoattractant was inhibited by HSS treatment. HSS suppressed TNF-α-induced nuclear factor kappa B (NF-κB) activation via preventing proteolytic degradation of inhibitor κB-α. HSS induced the production of heme oxygenase 1 via translocation of Nrf2 into the nucleus in TNF-α-treated HUVECs. Overall, HSS alleviated vascular inflammation through the downregulation of NF-κB activation and the upregulation of Nrf2 activation in TNF-α-induced HUVECs. These results indicate that HSS may be used as therapeutic agents for vascular inflammatory disorders. |
| first_indexed | 2024-12-20T08:18:24Z |
| format | Article |
| id | doaj.art-bae8d52181b64f74b48ac934be1ee18f |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-20T08:18:24Z |
| publishDate | 2019-03-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-bae8d52181b64f74b48ac934be1ee18f2022-12-21T19:47:04ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572019-03-0122111010.1186/s41240-019-0122-7Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cellsWi-Gyeong Gwon0Sang-Gil Lee1Jae-Il Kim2Young-Mog Kim3Seon-Bong Kim4Hyeung-Rak Kim5Department of Food Science and Nutrition, Pukyong National UniversityDepartment of Food Science and Nutrition, Pukyong National UniversityDepartment of Food Science and Nutrition, Pukyong National UniversityDepartment of Food Technology, Pukyong National UniversityDepartment of Food Technology, Pukyong National UniversityDepartment of Food Science and Nutrition, Pukyong National UniversityAbstract Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhibition of tumor necrosis factor (TNF)-α-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). We found that HSS suppressed the production of cell adhesion molecules such as intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 in TNF-α-induced HUVECs. Moreover, TNF-α-induced production of monocyte chemoattractant protein 1 and keratinocyte chemoattractant was inhibited by HSS treatment. HSS suppressed TNF-α-induced nuclear factor kappa B (NF-κB) activation via preventing proteolytic degradation of inhibitor κB-α. HSS induced the production of heme oxygenase 1 via translocation of Nrf2 into the nucleus in TNF-α-treated HUVECs. Overall, HSS alleviated vascular inflammation through the downregulation of NF-κB activation and the upregulation of Nrf2 activation in TNF-α-induced HUVECs. These results indicate that HSS may be used as therapeutic agents for vascular inflammatory disorders.http://link.springer.com/article/10.1186/s41240-019-0122-7Sargassum serratifoliumVascular inflammationNuclear factor-κBAdhesion moleculeHuman umbilical vein endothelial cell |
| spellingShingle | Wi-Gyeong Gwon Sang-Gil Lee Jae-Il Kim Young-Mog Kim Seon-Bong Kim Hyeung-Rak Kim Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells Fisheries and Aquatic Sciences Sargassum serratifolium Vascular inflammation Nuclear factor-κB Adhesion molecule Human umbilical vein endothelial cell |
| title | Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells |
| title_full | Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells |
| title_fullStr | Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells |
| title_full_unstemmed | Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells |
| title_short | Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells |
| title_sort | hexane fraction from the ethanolic extract of sargassum serratifolium suppresses cell adhesion molecules via regulation of nf κb and nrf2 pathway in human umbilical vein endothelial cells |
| topic | Sargassum serratifolium Vascular inflammation Nuclear factor-κB Adhesion molecule Human umbilical vein endothelial cell |
| url | http://link.springer.com/article/10.1186/s41240-019-0122-7 |
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